Gene expression profiling of human articular cartilage grafts generated by tissue engineering☆

Kaps, Christian; Frauenschuh, Simone; Endres, Michaela; Ringe, Jochen; Haisch, Andreas; Lauber, Jörg; Buer, Jan; Krenn, Veit; Häupl, Thomas; Burmester, Gerd‐Rüdiger; Sittinger, Michael

doi:10.1016/j.biomaterials.2006.02.017

Cited by 27 publications

(32 citation statements)

References 52 publications

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“…The activation of glucose transport would help cells obtain energy in order to maintain hypoxia condition. 4 B Focal adhesion function is also affected after treatment with nickel, which leads to the decrease of cell adhesion and proliferation. Extracellular matrix may be influenced, such as degradation of fibronectin (FN) and generation of collagen.…”

Section: Dna Damagementioning

confidence: 99%

“…Furthermore, the effect of biomaterials to cells on gene expression profiles could be investigated efficiently through this technique. Currently, microarray analysis utilized in the study of cell-biomaterial interaction involves a variety of cell types and different kinds of biomaterials, ranging from metal oxide materials [3], tissue engineering grafts [4], metal ions released from implant corrosion [5], etc. To understand the mechanisms of cells in response to different biomaterials, gene expression profiles were gained from microarray results, representative of a myriad of cellular activities, including cell adhesion, growth, differentiation and apoptosis.…”

Section: Introductionmentioning

confidence: 99%

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Genome-wide pathways analysis of nickel ion-induced differential genes expression in fibroblasts

Lü

Zhao

et al. 2010

Biomaterials

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Section: Dna Damagementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genome-wide pathways analysis of nickel ion-induced differential genes expression in fibroblasts

Lü

Zhao

et al. 2010

Biomaterials

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“…To address these questions, a few recent studies have started to explore the molecular basis of cell-biomaterial interactions by transcriptomic or proteomic methods (for review see (Gallagher et al, 2006)). In the context of cartilage engineering, however, only Kaps et al have examined the gene expression profiling of (human) articular chondrocytes that are cultured three-dimensionally in PGLA (polyglactin and polydioxanon) scaffolds with the aid of cDNA microarray (Kaps et al, 2006). They found that threedimensional assembly of dedifferentiated chondrocytes initiated the re-differentiation of cells that was accompanied by the expression of multiple players of TGF-b signaling pathway, including growth and differentiation factor-5 and inhibitor of differentiation-1.…”

Section: Discussionmentioning

confidence: 98%

“…Since both pathways are associated with chondrocyte proliferation and differentiation (Kaps et al, 2006;Minina et al, 2001), we inferred that the Hedgehog and TGF-b pathways played important roles in modulating the ECM accumulation in the CSC/DS/chitosan group. To attest this hypothesis, the expression of selected genes implicated in or activated by these pathways was measured by qRT-PCR.…”

Section: The Effect Of Csc/ds On Signal Transduction Pathways (Cdna Mmentioning

confidence: 98%

Co‐conjugating chondroitin‐6‐sulfate/dermatan sulfate to chitosan scaffold alters chondrocyte gene expression and signaling profiles

Chen

Chan

et al. 2008

Biotech & Bioengineering

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Co-conjugating chondroitin-6-sulfate (CSC) and dermatan sulfate (DS) to chitosan scaffolds improves chondrocyte differentiation and extracellular matrix (ECM) production. To further elucidate the cellular responses to CSC/DS conjugation, gene expression profiles for the rat chondrocytes cultured on the CSC/DS/chitosan and chitosan-only scaffolds were compared by reverse-transcription PCR (RT-PCR) and quantitative real-time RT-PCR (qRT-PCR). Our data unraveled that the CSC/DS/chitosan scaffold resulted in low-level expression of collagen I, IIA and X and potentiated the aggrecan, collagen II (including collagen IIB) and TIMP3 expression, but downregulated the decorin expression. Therefore CSC/DS/chitosan scaffold maintained the chondrocyte differentiation while minimized de-differentiation and hypertrophy. Furthermore, CSC/DS conjugation affected the expression of 11 genes implicated in 9 signaling pathways (as unveiled by cDNA microarray) and upregulated the expression of TGF-beta1, Sox9, BMP2, PTHrP and Ihh (as confirmed by qRT-PCR). These data suggested that the CSC/DS/chitosan scaffold potentiated the TGF-beta and Hedgehog pathways, which activated the expression of PTHrP and its downstream Sox9. The signals were transduced to elevate the expression of aggrecan, collagen II and TIMP3, and contributed to the well-differentiated chondrocyte phenotype. Altogether, this study for the first time elucidated the roles of GAGs-conjugated biomaterials in matrix production and breakdown, cellular differentiation and signal transduction at the molecular levels.

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“…Diverging from cell-biomaterial interactions, there is a continuously expanding field Oligonucleotide arrays were used in cartilage tissue engineering to explore the differences between de-and re-differentiation between native cartilage, in vitro expanded chondrocytes and three-dimensionally assembled chondrocyte cultures [65].…”

Section: Use Of Microarrays In Regenerative Medicinementioning

confidence: 99%

Examination of cell–host–biomaterial interactions via high-throughput technologies: A re-appraisal

2010

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Biomaterials are required to act harmoniously when exposed to the body or bodily fluids. Investigating cellular or in vivo phenotypic responses and protein adsorption to the material surface helps to determine the associated biocompatibility. Past limitations on progress in this field include time-consuming cell-based screening tools and a limited understanding of the complex nature of cell-biomaterial interactions.While high-throughput technologies by their nature are a rapid tool to derive meaning from multifaceted systems and, in recent years, the biomaterial community is beginning to take advantage of these technologies, the key observation in this Leading Opinion Paper is that the biomaterials community has been slow to accept these methods as an addition to their traditional experimentation workflow. The purpose of this paper is to review the definition and recent usage of high-throughput experiments in order to examine biomaterial interactions at the cellular and wider host level, especially as they become more relevant within the biomaterials arena encapsulating tissue engineering, gene, drug and stem cell delivery systems. The technologies under focus include rapid cell-based screening, transcriptomics and proteomics.

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Gene expression profiling of human articular cartilage grafts generated by tissue engineering☆

Cited by 27 publications

References 52 publications

Genome-wide pathways analysis of nickel ion-induced differential genes expression in fibroblasts

Genome-wide pathways analysis of nickel ion-induced differential genes expression in fibroblasts

Co‐conjugating chondroitin‐6‐sulfate/dermatan sulfate to chitosan scaffold alters chondrocyte gene expression and signaling profiles

Examination of cell–host–biomaterial interactions via high-throughput technologies: A re-appraisal

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