Genome-wide pathways analysis of nickel ion-induced differential genes expression in fibroblasts

Lü, Xiaoying; Lu, Hui-Qin; Zhao, Lei; Yang, Yamin; Zhang, Lu

doi:10.1016/j.biomaterials.2009.12.044

Cited by 38 publications

(32 citation statements)

References 33 publications

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“…Previous studies found that even though no cytotoxicity was detected by MTT assay, the expression of massive candidate genes had been dramatically altered [5]. These results suggest that although the cellular response to the nickel toxicity was not obvious in a short interaction period, there has already been a negative effect at the molecular level.…”

Section: Discussionmentioning

confidence: 82%

“…ISR has been characterized by a process called neointimal hyperplasia, a sequential event of inflammation, granulation, extracellular matrix remodeling, and vascular smooth muscle cells (VSMCs) proliferation and migration [4], [5]. Unlike cardiac or skeletal muscle cells, VSMCs are not terminally differentiated rather than being able to continuously modulate their phenotype.…”

Section: Introductionmentioning

confidence: 99%

“…This latter event is primarily influenced by endothelial cells (ECs). Previous studies on the post-angioplasty follow-ups suggested that, lack of EC layer, VSMCs would acquire a synthetic phenotype, leading to extensive migration, proliferation, and matrix synthesis that contribute to restenosis [5], [6]. In addition, damage to the EC layer during the stent implantation can also lead to neointimal hyperplasia and eventually to ISR [7].…”

Section: Introductionmentioning

confidence: 99%

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Reduction of In-Stent Restenosis Risk on Nickel-Free Stainless Steel by Regulating Cell Apoptosis and Cell Cycle

Pan

Zhou

et al. 2013

PLoS ONE

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High nitrogen nickel-free austenitic stainless steel (HNNF SS) is one of the biomaterials developed recently for circumventing the in-stent restenosis (ISR) in coronary stent applications. To understand the ISR-resistance mechanism, we have conducted a comparative study of cellular and molecular responses of human umbilical vein endothelial cells (HUVECs) to HNNF SS and 316L SS (nickel-containing austenitic 316L stainless steel) which is the stent material used currently. CCK-8 analysis and flow cytometric analysis were used to assess the cellular responses (proliferation, apoptosis, and cell cycle), and quantitative real-time PCR (qRT-PCR) was used to analyze the gene expression profile of HUVECs exposed to HNNF SS and 316L SS, respectively. Flow cytometry analysis revealed that 316L SS could activate the cellular apoptosis more efficiently and initiate an earlier entry into the S-phase of cell cycle than HNNF SS. At the molecular level, qRT-PCR results showed that the genes regulating cell apoptosis and autophagy were overexpressed on 316L SS. Further examination indicated that nickel released from 316L SS triggered the cell apoptosis via Fas-Caspase8-Caspase3 exogenous pathway. These molecular mechanisms of HUVECs present a good model for elucidating the observed cellular responses. The findings in this study furnish valuable information for understanding the mechanism of ISR-resistance on the cellular and molecular basis as well as for developing new biomedical materials for stent applications.

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Section: Discussionmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Reduction of In-Stent Restenosis Risk on Nickel-Free Stainless Steel by Regulating Cell Apoptosis and Cell Cycle

Pan

Zhou

et al. 2013

PLoS ONE

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“…The global gene expression analysis using the microarray technique could gain profiling information of nanomaterial-cell interactions [25,32,33]. …”

Section: Introductionmentioning

confidence: 99%

Genotoxicity and molecular response of silver nanoparticle (NP)-based hydrogel

Takemura

et al. 2012

J Nanobiotechnol

124

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BackgroundSince silver-nanoparticles (NPs) possess an antibacterial activity, they were commonly used in medical products and devices, food storage materials, cosmetics, various health care products, and industrial products. Various silver-NP based medical devices are available for clinical uses, such as silver-NP based dressing and silver-NP based hydrogel (silver-NP-hydrogel) for medical applications. Although the previous data have suggested silver-NPs induced toxicity in vivo and in vitro, there is lack information about the mechanisms of biological response and potential toxicity of silver-NP-hydrogel.MethodsIn this study, the genotoxicity of silver-NP-hydrogel was assayed using cytokinesis-block micronucleus (CBMN). The molecular response was studied using DNA microarray and GO pathway analysis.Results and discussionThe results of global gene expression analysis in HeLa cells showed that thousands of genes were up- or down-regulated at 48 h of silver-NP-hydrogel exposure. Further GO pathway analysis suggested that fourteen theoretical activating signaling pathways were attributed to up-regulated genes; and three signal pathways were attributed to down-regulated genes. It was discussed that the cells protect themselves against silver NP-mediated toxicity through up-regulating metallothionein genes and anti-oxidative stress genes. The changes in DNA damage, apoptosis and mitosis pathway were closely related to silver-NP-induced cytotoxicity and chromosome damage. The down-regulation of CDC14A via mitosis pathway might play a role in potential genotoxicity induced by silver-NPs.ConclusionsThe silver-NP-hydrogel induced micronuclei formation in cellular level and broad spectrum molecular responses in gene expression level. The results of signal pathway analysis suggested that the balances between anti-ROS response and DNA damage, chromosome instability, mitosis inhibition might play important roles in silver-NP induced toxicity. The inflammatory factors were likely involved in silver-NP-hydrogel complex-induced toxic effects via JAK-STAT signal transduction pathway and immune response pathway. These biological responses eventually decide the future of the cells, survival or apoptosis.

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“…It should be noted, however, that this deficiency in a toxicological profile might be limited to the specific particles and organism under study. [56] and [57]. Nickel-titanium (NiTi) is widely used in the medical field including in orthodontics [58], root canals [59] and colon surgery [60].…”

Section: Use Of Microarrays In Assessing the Response Of Cells To Metmentioning

confidence: 99%

Examination of cell–host–biomaterial interactions via high-throughput technologies: A re-appraisal

2010

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Biomaterials are required to act harmoniously when exposed to the body or bodily fluids. Investigating cellular or in vivo phenotypic responses and protein adsorption to the material surface helps to determine the associated biocompatibility. Past limitations on progress in this field include time-consuming cell-based screening tools and a limited understanding of the complex nature of cell-biomaterial interactions.While high-throughput technologies by their nature are a rapid tool to derive meaning from multifaceted systems and, in recent years, the biomaterial community is beginning to take advantage of these technologies, the key observation in this Leading Opinion Paper is that the biomaterials community has been slow to accept these methods as an addition to their traditional experimentation workflow. The purpose of this paper is to review the definition and recent usage of high-throughput experiments in order to examine biomaterial interactions at the cellular and wider host level, especially as they become more relevant within the biomaterials arena encapsulating tissue engineering, gene, drug and stem cell delivery systems. The technologies under focus include rapid cell-based screening, transcriptomics and proteomics.

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Genome-wide pathways analysis of nickel ion-induced differential genes expression in fibroblasts

Cited by 38 publications

References 33 publications

Reduction of In-Stent Restenosis Risk on Nickel-Free Stainless Steel by Regulating Cell Apoptosis and Cell Cycle

Reduction of In-Stent Restenosis Risk on Nickel-Free Stainless Steel by Regulating Cell Apoptosis and Cell Cycle

Genotoxicity and molecular response of silver nanoparticle (NP)-based hydrogel

Examination of cell–host–biomaterial interactions via high-throughput technologies: A re-appraisal

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