Gene transfer in chinook salmon (Oncorhynchus tshawytscha) by electroporating sperm in the presence of pRSV-lacZ DNA

Sin, F. Y. T.; Bartley, Anne; Walker, Seumas P.; Sin, Iris L.; Symonds, Jane E.; Hawke, Lindsey G.; Hopkins, C. L.

doi:10.1016/0044-8486(93)90123-g

Cited by 39 publications

(31 citation statements)

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“…Khoo et al (1992) reported that simply incubating sperm with foreign DNA yielded transgenic zebra fish (Brachydanio rerio). Use of procedures similar to those of Koo et al (1992) failed to produce transgenic rainbow trout (Oncorhynchus mykiss) (Chourout and Perrot, 1992), common carp (Cyprinus carpio), African catfish (Clarias gariepinus), tilapia (Oreochromis niloticus) (Müller et al, 1992), or Chinook salmon (Oncorhynchus tshawytscha) (Sin et al, 1993). However, Müller et al (1992) and Sin et al (1993) did find electroporation enhanced DNA/ sperm association, as did several subsequent studies.…”

Section: Introductionmentioning

confidence: 99%

Association of Foreign DNA with Sperm of Gilthead Seabream, Sparus aurata, After Sonication, Freezing, and Dimethyl Sulfoxide Treatments

1999

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: The association of foreign DNA with gilthead seabream (Sparus aurata) sperm was enhanced relative to simple coincubation by sonication, freezing, dimethyl sulfoxide and polyethylene glycol treatments. Sonication yielded the strongest dot blot signals, equivalent to 250 to 380 foreign DNA copies per spermatozoa. We are unaware of previous reports attempting to associate DNA with ultrasound for fish or elsewhere. However, no or negligible foreign DNA was evident in 1- and 2-day-old fish larvae resulting from eggs fertilized with sonicated or frozen sperm.

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Section: Introductionmentioning

confidence: 99%

Association of Foreign DNA with Sperm of Gilthead Seabream, Sparus aurata, After Sonication, Freezing, and Dimethyl Sulfoxide Treatments

1999

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“…Pada awalnya, metoda ini dikembangkan untuk kultur sel; namun demikian teknik ini dapat juga diaplikasikan untuk telur dan sperma ikan. Teknik eletroforesis telah digunakan dalam beberapa spesies ekonomis penting seperti channel catfish, carp (Powers et al 1992), dan salmon (Sin et al 1993;Symonds et al 1994). Powers et al (1992) memproduksi ikan transgenic channel catfish dan carp dengan melakukan elektroforesis menggunakan telur yang telah dibuahi.…”

Section: Elektroforesisunclassified

Aplication of Gene Transfer in Aquaculture

Ali

Yoshizaki

Carman

et al. 2007

Jurnal Akuakultur Indonesia

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Recently, global food security has become a hot issue by the public in national as well as out of the country. Aquacultural output will need to be increased several fold in order to meet the rising demands for fish in coming years as the increasing of mankind population. The intensity and capacity of production is expected to increase using biotechnology approach. One of the advances biotechnologies that expected to be a powerful approach for aquaculture development is transgenic technique. This technique has been applied to several commercially valuable species. This review describes various techniques of gene transfer, persistence and expression of transferred gene, application and future aspect of gene transfer research in aquaculture.Key words: Gene transfer, gene expression, biotechnology, aquaculture ABSTRAKSaat ini, keamanan pangan telah menjadi isu hangat di masyarakat baik di dalam maupun di luar negeri. Produksi akuakultur diharapkan dapat ditingkatkan beberapa kali lipat untuk memenuhi kebutuhan pangan berupa ikan dimasa-masa mendatang akibat peningkatan populasi manusia. Intensitas dan kapasitas produksi diharapkan meningkat dengan menggunakan pendekatan bioteknologi. Salah satu teknik modern yang diduga akan menjadi sarana yang berguna dalam pengembangan akuakultur adalah teknologi transfer gen. Teknik ini telah diaplikasikan pada spesies-spesies yang memiliki nilai ekonomis. Ulasan ini menggambarkan variasi metode transfer gen, persistensi dan ekspressi dari gen yang ditransfer, aplikasi dan prospeknya ke depan dari penelitian transfer gen dalam akuakultur.Kata kunci : transfer gen, ekspressi gen, bioteknologi, akuakultur PENDAHULUANKeamanan pangan telah menjadi isu utama saat ini baik di dalam maupun di luar negeri. Dengan perkiraan jumlah populasi dunia mencapai 11 milyar pada tahun 2050, sektor pertanian (termasuk perikanan) ditantang untuk bisa melipatgandakan produksi pangan di tahun 2025 dan tiga kali lipat di tahun 2050, dengan kondisi lahan dan air per kapita berkurang dan tantangan kondisi lingkungan meningkat. Peningkatan produksi perikanan, dalam hal ini seafood, diperlukan sekitar 7 kali lipat di tahun 2020 (Hew & Fletcher 1997). Produksi pertanian yang berbasis lahan, dalam arti luasan dan output per hektar, telah mencapai titik maksimum dengan melakukan pemupukan dan selektif breeding. Hal tersebut diperburuk oleh bertambah jeleknya kualitas tanah, air dan udara, yang disebabkan oleh perubahan iklim global, penggundulan lahan, polusi,dan industrialisasi .Perkembangan ilmu biologi molekuler akhir-akhir ini telah memungkinkan dengan mudah membuat klon suatu gen yang diinginkan. Pada tahun 1980, Gordon et al. (1980) melaporkan keberhasilan memindahkan gen ke tikus dengan cara menyuntikkannya ke dalam pronukleus telur yang sudah dibuahi. Tikus tersebut selanjutnya dinamakan "tikus transgenik". Dengan menggunakan teknik ini, suatu gen yang menkodekan karakter tertentu yang diinginkan dapat diintroduksi ke suatu individu. Sekali gen asing terintegrasi ke dalam genom resipien, gen te...

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“…A GAL4 system was used for transgenic expression in zebrafish (Koster and Fraser, 2001). IPTG is an inducer of the lactose operon that has also been used to induce transgene expression in mammalian cells since it does not produce any adverse effects (Lin et al, 1994b;Sin et al, 1993). The commercial vector pCMVLacI, for example, produces the repressor that blocks transcription of its specific promoter in the absence of IPTG (Ryding et al, 2001).…”

Section: Inducible Expression By Heterologous Systemsmentioning

confidence: 99%

Review. Fish as biofactories: indicible genetic systems and gene targeting

Rocha¹,

Ruiz²,

Estepa³

et al. 2003

Span. j. agric. res.

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The production of recombinant human proteins for pharmaceutical products in transgenic animals is progressingslowly. Compared to mammals, fish offer easier genetic manipulation since they produce hundreds of eggs per femaleand because of the independent development of fish embryos. Traditional methods in the past have employedfertilized egg injections with transgene constructions containing non-specific promoters and random genome integration.In fish, these methods have resulted in mosaicism and extrachromosomal expression. Inducible systems tocontrol the temporal and tissue specific expression and gene targeting methodologies to increase efficiency and controlof the locus of insertion of the transgene are, therefore, desirable. All these techniques incorporate stem cell manipulation,classification, selection and/or nuclear transplant of cell lines or stem cells. Some of these systemsinitially developed for mammalian cells are now available to be tested in fish. A large part of this field still remainsunexplored.

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Gene transfer in chinook salmon (Oncorhynchus tshawytscha) by electroporating sperm in the presence of pRSV-lacZ DNA

Cited by 39 publications

References 38 publications

Association of Foreign DNA with Sperm of Gilthead Seabream, Sparus aurata, After Sonication, Freezing, and Dimethyl Sulfoxide Treatments

Association of Foreign DNA with Sperm of Gilthead Seabream, Sparus aurata, After Sonication, Freezing, and Dimethyl Sulfoxide Treatments

Aplication of Gene Transfer in Aquaculture

Review. Fish as biofactories: indicible genetic systems and gene targeting

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