General principles of conducting preclinical toxicology studies of antiparasitic drugs for veterinary use

Arisov, M. V.; Urazaev, D. N.; Качанова, Е. О.; Павлова, А.

doi:10.1088/1755-1315/548/4/042042

Cited by 4 publications

(2 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Determination of acute toxicity is the first stage in the study of the toxic properties of the Drugs. The purpose of which is to obtain information about the dangers of the investigational drug in the conditions of short-term action and, as a result, provides for obtaining data on the lethal dose and the clinical picture [15].…”

Section: Discussionmentioning

confidence: 99%

Determination of toxicological characteristics of a complex antiemeric drug based on maduramycin and nicarbazine

Dotsenko

Vashchyk

Zakhariev

et al. 2021

SR: BS

View full text Add to dashboard Cite

The aim: to determine the parameters of acute toxicity of the preparative form of an antiemeric agent based on maduramycin and nicarbazine for white mice, white rats and guinea pigs with a single oral administration. Materials and methods. Determination of acute toxicity of the formulation by oral administration was performed on 48 adult male mice, 48 adult nonlinear male rats, 48 adult male guinea pigs. To conduct an experiment on the principle analogues were formed seven experimental and one control group, 6 animal each. The dose of the formulation was calculated individually based on body weight values. It should be noted that the total volume of the emulsion of the formulation administered orally is not exceeded 1.0 cm3per 100 gram b. w. Results. Toxicometric parameters of the formulation were calculated by the method of least squares for probit analysis of mortality curves. It was found that the LD50 of the preparative form of antiemeric agent for white mice for a single oral administration is 238.05±28.08 mg/kg, LD16 – 128.71 mg/kg, LD84 – 347.39 mg/kg, LD100 - 402, 06 mg/kg body weight, respectively. LD50 of the preparative form of antiemeric agent for white rats with a single oral administration is 260.51±28.83 mg/kg, LD16 – 148.39 mg/kg, LD84 – 372.65 mg/kg, LD100 – 428.71 mg/kg body weight, respectively. LD50 of the preparative form of antiemeric agent for guinea pigs for a single oral administration is 275±21.12 mg/kg, LD16 – 201.74 mg/kg, LD84 – 348.25 mg/kg, LD100 – 384.88 mg/kg body weight body respectively. Conclusions. According to SOU 85.2-37-736: 2011 "Veterinary drugs. Determination of acute toxicity” preparative form of a complex antiemeric agent based on maduramycin and nicarbazine on the degree of toxicity can be attributed to moderately dangerous substances (3rd hazard class)

show abstract

Section: Discussionmentioning

confidence: 99%

Determination of toxicological characteristics of a complex antiemeric drug based on maduramycin and nicarbazine

Dotsenko

Vashchyk

Zakhariev

et al. 2021

SR: BS

View full text Add to dashboard Cite

show abstract

“…For an intensive relocation, the repression of the creatures is very buzzing, with signs of a loss of control and coordination of rucks. In some cases, they observed one by one cloned convulsions, animals move their limbs unnaturally [9].…”

Section: Introductionmentioning

confidence: 99%

The effect of a formulation based on maduramycin and nicarbazine on rats and broiler chickens in a subacute experiment

Dotsenko

Romanko

Vashchyk

et al. 2021

SR: BS

View full text Add to dashboard Cite

The aim: to determine the effect of a preparative form of an antiemeric agent based on maduramycin and nicarbazine on the body of white rats and broiler chickens under the conditions of a subacute experiment. Materials and methods. The formulation based on maduramycin and nicarbazine was administered to rats and broiler chickens with feed for 28 days. One control and three experimental groups were formed for the experiment: Group I – animals received a complete diet without admixture of the formulation (control group), Group II – animals that were administered the formulation (by the amount of active substances) at a dose of 5.0 mg / kg, III – 25.0 mg / kg and IV – 50.0 mg / kg of feed, respectively. In order to establish the toxic effect of the formulation on the body of experimental birds on 7, 14, 28 days of the experiment and 7 days after discontinuation of the formulation, 5 heads from each group were killed under light ether anesthesia, blood samples were taken for hematological and biochemical studies. Results. During the study of the general clinical condition of rats and broiler chickens of the experimental groups, no significant changes in behavior and appearance were detected, compared with the control. Hematological parameters of broiler chickens it was found that the receipt of the formulation with feed at doses of 5, 25 and 50 mg/kg body weight, causes a decrease in hemoglobin and an increase in hematocrit in broiler chickens on day 7 of the experiment. It was found that the changes are reversible, on the 28th day of the experiment the value of these indicators was within the physiological norm. In the study of hematological and biochemical parameters of rats, it was found that the receipt of the formulation with food in doses of 25.0 and 50.0 mg/kg for 28 days, causes a decrease in hemoglobin and increase hematocrit, total protein, albumin, creatinine and urea in male rats on the 7th day of the experiment. Conclusions. Under conditions of repeated oral administration of the preparation mixture to rats and poultry at doses of 5, 25 and 50 mg/kg body weight in the absence of clinical signs of poisoning, insignificant fluctuations in hematological and biochemical parameters were identified

show abstract

Study of the acute toxicity of the medicinal product for veterinary use Iverbutan

Indyuhova

Arisova

Belykh

et al. 2021

Ross. parazitol. ž..

View full text Add to dashboard Cite

The purpose of the research is to study the acute oral toxicity of the medicinal product for veterinary use Iverbutan, intended for the treatment and prevention of arachnoentomoses and nematodoses of poultry.Materials and methods. The studies were carried out on 30 outbred male rats weighing 210-240 g and 60 mice weighing 18–21 g. The animals were divided into experimental and control groups. The drug was administered once without dilution in the form of the provided solution using an intragastric tube. Doses of 2000, 4000, 6000, 8000 and 10 000 mg/kg were tested on mice, and on rats – 10 000, 8000, 5000, 4000 mg/kg. The animals of the control groups were injected with drinking water. Within 14 days after a single dose of the drug, the physiological state and behavior of animals, possible death, as well as the manifestation of symptoms of intoxication were monitored. The control of the body weight of the animals of the experimental and control groups was carried out on the day of the experiment (before drug administration), as well as on the 1st, 3rd, 7th, 9th and 14th days.Results and discussion. It was found that after oral administration of iverbutan to experimental animals, the average lethal dose, calculated by the Kerber method, was 5600 mg/kg of body weight in mice and 7000 mg/kg of body weight in rats (hazard class 4 according to GOST 12.1.007-76). The average lethal dose, calculated by the Miller and Tainter method, was 5292.0±1058.6 (4233.4÷6350.6) mg/kg of body weight in mice and 6463.2±1496.9 (4966.3÷7960.1) mg/kg of body weight of rats (hazard class 3 according to GOST 12.1.007-76), which indicates species sensitivity.

show abstract

General principles of conducting preclinical toxicology studies of antiparasitic drugs for veterinary use

Cited by 4 publications

References 2 publications

Determination of toxicological characteristics of a complex antiemeric drug based on maduramycin and nicarbazine

Determination of toxicological characteristics of a complex antiemeric drug based on maduramycin and nicarbazine

The effect of a formulation based on maduramycin and nicarbazine on rats and broiler chickens in a subacute experiment

Study of the acute toxicity of the medicinal product for veterinary use Iverbutan

Contact Info

Product

Resources

About