The domain of Automatic Speaker Verification (ASV) is blooming with growing developments in feature engineering and artificial intelligence. Inspite of this, the system is liable to spoofing attacks in the form of synthetic or replayed speech. The difficulty in detecting synthetic speech is due to recent advancements in the Voice conversion and Text-to-speech systems which produce natural, indistinguishable speech. To prevent such attacks, there is a need to develop robust spoof detection systems. In order to achieve this goal, we are proposing estimation of Glottal Flow Parameters (GFP) from speech of genuine speech and synthetic spoof samples. The GFP are further parameterized using time, frequency and Liljencrants-Fant (LF) models. Along with GFP features, the Linear Prediction Cepstrum Co-efficient (LFCC) and statistical parameters are computed. The GFP features are investigated to prove their usefulness in detecting spoofed and genuine speech. The ASV spoof 2019 corpus is used to test the framework and evaluated against the baseline models. The proposed spoof detection framework produces an Equal Error Rate (EER) of 2.39% and tandem Detection Cost Function (t-DCF) of 0.0562 which is found to be better than the state-of-the art technique.