2020
DOI: 10.21203/rs.3.rs-125471/v1
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Generation of heritable double muscle buttocks rabbits via myostatin mutation with CRISPR/Cas9 system

Abstract: Background Myostatin (MSTN) is a member of the transforming growth factor (TGF-β) superfamily and is considered to be a negative regulator that inhibits muscle development and regeneration, inactivity of MSTN gene may affect the development and regeneration of muscle in a few of animals. Results In the present study, the muscle mass negative regulator gene myostatin (MSTN) was knocked out at two novel sits in exon3, and the function of these mutation was determined in the offspring rabbits. The typical doubl… Show more

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Cited by 3 publications
(5 citation statements)
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“…Reports on targeting the leporine MSTN gene (OMIA 000683-9986) have been published after the emergence of the CRISPR/Cas9 system, and live MSTN -edited rabbits were reported (Table ). Both CRISPR/Cas9-based knockout and CBE-based induction of premature stop codons , were applied to produce MSTN -disrupted rabbits. In a recent report, the body weight of CRISPR/Cas9-mediated MSTN -disrupted rabbits was significantly higher than that of WT rabbits, and a heritable prominent double-muscled buttock phenotype was shown in subsequent generations .…”
Section: Targeting the Mstn Gene In Rabbitsmentioning
confidence: 99%
See 2 more Smart Citations
“…Reports on targeting the leporine MSTN gene (OMIA 000683-9986) have been published after the emergence of the CRISPR/Cas9 system, and live MSTN -edited rabbits were reported (Table ). Both CRISPR/Cas9-based knockout and CBE-based induction of premature stop codons , were applied to produce MSTN -disrupted rabbits. In a recent report, the body weight of CRISPR/Cas9-mediated MSTN -disrupted rabbits was significantly higher than that of WT rabbits, and a heritable prominent double-muscled buttock phenotype was shown in subsequent generations .…”
Section: Targeting the Mstn Gene In Rabbitsmentioning
confidence: 99%
“…Both CRISPR/Cas9-based knockout and CBE-based induction of premature stop codons , were applied to produce MSTN -disrupted rabbits. In a recent report, the body weight of CRISPR/Cas9-mediated MSTN -disrupted rabbits was significantly higher than that of WT rabbits, and a heritable prominent double-muscled buttock phenotype was shown in subsequent generations . Additionally, the expected double-muscling phenotype was observed in CBE-mediated MSTN -disrupted rabbits relative to their WT counterparts .…”
Section: Targeting the Mstn Gene In Rabbitsmentioning
confidence: 99%
See 1 more Smart Citation
“…Inactivation, through C-terminal deletion, of the MSTN gene was shown to determine a two- to three-fold increase in the skeletal muscle mass in mutant compared to wild-type mice [ 5 ]. The MSTN -knocked out rabbits were shown to generate a heritable phenotype with hyperplasia and hypertrophy of the muscle fibers, which could be valuable in improving rabbits’ meat production [ 6 ]. MSTN is, indeed, among the most studied genes in meat-producing animals.…”
Section: Introductionmentioning
confidence: 99%
“…The major impediment in China's buffalo industry included poor reproductive performance and milk production of local buffalo as compared to dairy cattle, so major efforts were directed towards improving the buffalo herd size to increase reproductive efficiency through utilizing reproductive technologies [8,[10][11][12][13], to identify the genetic markers and genes, which were associated with phenotypic variations [14][15][16] of desirable traits [17][18][19]. In China, the information related to buffalo breeding is still limited regarding molecular breeding techniques.…”
Section: Introductionmentioning
confidence: 99%