Generation of Heritable Prominent Double Muscle Buttock Rabbits via Novel Site Editing of Myostatin Gene Using CRISPR/Cas9 System

Zheng, Yalin; Zhang, Yu; Wu, Liyan; Riaz, Hasan; Li, Zhipeng; Shi, Dongquan; Rehman, Saif Ur; Liu, Qingyou; Cui, Kuiqing

doi:10.3389/fvets.2022.842074

Cited by 7 publications

(2 citation statements)

References 59 publications

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“…In chickens, the alteration of genes involved in embryonic development has led to improved hatchability [ 71 ]. The change in bone development-related genes has also led to improved skeletal health and meat quality in pigs [ 72 ]. Besides, a study on the CRISPR/Cas9 method showed that heritable double muscle buttocks in rabbits could be achieved through myostatin mutation, which was useful for producing rabbit meat [ 73 ].…”

Section: Resultsmentioning

confidence: 99%

Advancement of animal and poultry nutrition: Harnessing the power of CRISPR-Cas genome editing technology

Mishu,

Nath,

Sohidullah

et al. 2024

J Adv Vet Anim Res

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CRISPR-associated proteins and clustered regularly interspaced short palindromic repeats (CRISPR-Cas) technology has emerged as a groundbreaking advancement in animal and poultry nutrition to improve feed conversion efficiency, enhance disease resistance, and improve the nutritional quality of animal products. Despite significant advancements, there is a research gap in the systematic understanding and comprehensive use of the CRISPR-Cas method in animal and poultry nutrition. The purpose of this study is to elucidate the latest advancements in animal and poultry nutrition through CRISPR-Cas genome editing technology, focusing on gene manipulation in metabolism, immunity, and growth. Following preferred reporting items in meta-analysis and systematic reviews guidelines, we conducted a systematic search using several databases, including Scopus, PubMed, and Web of Science, until May 2024, and finally, we included a total of 108 articles in this study. This article explores the use of the CRISPR-Cas system in the advancement of feed additives like probiotics and enzymes, which could reduce the use of antibiotics in animal production. Furthermore, the article discusses ethical and regulatory issues related to gene editing in animal and poultry nutrition, including concerns about animal welfare, food safety, and environmental impacts. Overall, the CRISPR-Cas system holds substantial promise to overcome the challenges in modern animal agriculture. By enriching the nutritional quality of animal products, increasing disease resistance, and improving feed efficiency, it offers sustainable and cost-effective solutions that can revolutionize animal and poultry nutrition

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Section: Resultsmentioning

confidence: 99%

Advancement of animal and poultry nutrition: Harnessing the power of CRISPR-Cas genome editing technology

Mishu,

Nath,

Sohidullah

et al. 2024

J Adv Vet Anim Res

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“…MSTN-null mice showed a dramatic and widespread increase in skeletal muscle mass [56]. Other studies had showed that MSTN gene has effects on skeletal muscle development in cattles [57], goats [58], pigs [59], and rabbits [60,61].…”

Section: Degs Analyzed At Four Stagesmentioning

confidence: 99%

Comparative Transcriptome Profiling of Skeletal Muscle From Fujian White Rabbit (Oryctolagus cuniculus) at Different Growth Stages Using RNA-Seq

Chen¹,

Sang²,

Sun³

et al. 2023

Preprint

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In China, the demand for rabbit meat is also increasing, and the production for rabbit meat is important. However, the underlying mechanisms of regulate skeletal muscle growth and development in rabbits remain unclear. The purpose of this study was to identify candidate genes related to skeletal muscle growth in rabbits and explore their potential regulatory mechanisms. RNA-seq technique was used to compare the differences of skeletal muscle transcriptome in Fujian white rabbits at different developmental stages (day 20 and 26 of embryos and birth 1, 30 and 60-day-olds) with a total of 25 individual selected. A total of 9737 DEGs that were acquired from the five groups were annotated into three ontologies of the GO database: biological process (BP), cell component (CC), and molecular function (MF). For KEGG analysis, there are 8249 genes were enriched in 1148 pathways, of which 67 pathways were significantly enriched, mainly in Hypertrophic cardiomyopathy (HCM), the PPAR signaling pathway, MAPK signaling pathway. Among all comparison groups, SE was the most abundant AS event. Real-time PCR verified that the expression patterns of differential genes were consistent with the transcriptome sequencing results. These results will provide a molecular regulation mechanism of muscle growth and development in Fujian white rabbits, and should serve an important theoretical basis for improving meat performance and growth rate of Chinese local meat rabbit breeds.

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When Less Is More: Targeting the Myostatin Gene in Livestock for Augmenting Meat Production

Kalds

Zhou

Huang

et al. 2023

J. Agric. Food Chem.

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How to increase meat production is one of the main questions in animal breeding. Selection for improved body weight has been made and, due to recent genomic advances, naturally occurring variants that are responsible for controlling economically relevant phenotypes have been revealed. The myostatin (MSTN) gene, a superstar gene in animal breeding, was discovered as a negative controller of muscle mass. In some livestock species, natural mutations in the MSTN gene could generate the agriculturally desirable double-muscling phenotype. However, some other livestock species or breeds lack these desirable variants. Genetic modification, particularly gene editing, offers an unprecedented opportunity to induce or mimic naturally occurring mutations in livestock genomes. To date, various MSTN-edited livestock species have been generated using different gene modification tools. These MSTN gene-edited models have higher growth rates and increased muscle mass, suggesting the high potential of utilizing MSTN gene editing in animal breeding. Additionally, post-editing investigations in most livestock species support the favorable influence of targeting the MSTN gene on meat quantity and quality. In this Review, we provide a collective discussion on targeting the MSTN gene in livestock to further encourage its utilization opportunities. It is expected that, shortly, MSTN gene-edited livestock will be commercialized, and MSTN-edited meat will be on the tables of ordinary customers.

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Generation of Heritable Prominent Double Muscle Buttock Rabbits via Novel Site Editing of Myostatin Gene Using CRISPR/Cas9 System

Cited by 7 publications

References 59 publications

Advancement of animal and poultry nutrition: Harnessing the power of CRISPR-Cas genome editing technology

Advancement of animal and poultry nutrition: Harnessing the power of CRISPR-Cas genome editing technology

Comparative Transcriptome Profiling of Skeletal Muscle From Fujian White Rabbit (Oryctolagus cuniculus) at Different Growth Stages Using RNA-Seq

When Less Is More: Targeting the Myostatin Gene in Livestock for Augmenting Meat Production

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