Generation of Janus alginate hydrogel particles with magnetic anisotropy for cell encapsulation

Zhao, Libo; Pan, Liqing; Zhang, K.; Guo, Shishang; Liu, W.; Wang, Yu; Chen, Y.; Zhao, Xingzhong; Chan, Helen L. W.

doi:10.1039/b907478c

Cited by 112 publications

(92 citation statements)

References 27 publications

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“…4 showed the relationships between the droplet size and the flow rates of dispersed phase and continuous phase. Under a dynamic balance, the relationship Q d = V d f d holds, where Q d is the flux of the dispersed phase, V d is the volume of the droplets, and f d is the breakup rate of the disperse phase (Nisisako et al 2006;Zhao et al 2009). For example, to prepare smaller droplets, we increased the continuous phase, Q c , while fixed the flux of the Q d .…”

Section: Formation Of Monodisperse Dropletsmentioning

confidence: 99%

“…By using droplet microfluidics, several groups have prepared monodisperse BPM Tan and Takeuchi 2007;Dendukuri and Doyle 2009;Xu et al 2005Xu et al , 2009Oh et al 2008;Huang et al 2008;Yeh and Lin 2009;Hung et al 2010). Several monodisperse BPM of mixed drug have been successfully fabricated by droplet microfluidics, including poly(D, L-lactide-co-glycolide) (PLGA) (Xu et al 2009), alginate (Zhao et al 2009), chitosan (Gong et al 2009), and others Shum et al 2008;Nisisako et al 2006).…”

Section: Introductionmentioning

confidence: 98%

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A modified microfluidic chip for fabrication of paclitaxel-loaded poly(l-lactic acid) microspheres

Liang

Zhang

et al. 2011

Microfluid Nanofluid

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In this article, we present a simple PDMS surface modification method based on poly(vinyl alcohol)/glycerol (PVA/Gly) solution immersion, self-assembled absorption, and heat treatment. The results of contact angle and ATR-FTIR demonstrate the superhydrophilic surface in modified PDMS. It can allow for the stable production of monodisperse droplet in a highly reproducible manner. In addition, we demonstrate the fabrication of monodisperse paclitaxel (PTX) loaded poly(L-lactic acid) (PLLA) microspheres on this kind of modification chip with solvent evaporation. The PLLA microspheres can be adjusted to a range of different sizes depending on the system flow rate. Determination of microsphere size is carried out by optical microscopy and image analysis to reveal less than 4% variation in microsphere size. Compared with the results of published papers, the presented data demonstrate that PTX-loaded PLLA microspheres show good physical properties (spherical and discrete), high-drug loading, encapsulation efficiency, a small initial burst, and sustained-release behavior due to outstanding monodispersity. With the characteristic to prepare high-quality, monodisperse, biodegradable microspheres, the versatile and simple microfluidic method facilitates the development of more reliable and reproducible drug delivery systems, which have great potential to benefit pharmaceutical and biological applications.

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Section: Formation Of Monodisperse Dropletsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

A modified microfluidic chip for fabrication of paclitaxel-loaded poly(l-lactic acid) microspheres

Liang

Zhang

et al. 2011

Microfluid Nanofluid

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“…One of the potential applications that receive much concern is the assembly of these particles into complex structure. Under the inspiration of cell membrane structure, a kind of micro-particle possessing amphiphilic property has been prepared in microfluidic device (Nie et al 2006;Dendukuri et al 2007;Zhao et al 2009). These particles with one part hydrophobic and the other part hydrophilic behave differently from isotropic particles at the water/oil interface (Dendukuri et al 2007).…”

Section: Introductionmentioning

confidence: 99%

A novel method for generation of amphiphilic PDMS particles by selective modification

Zhao

et al. 2010

Microfluid Nanofluid

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We reported a novel method to fabricate both spherical and nonspherical PDMS (Polydimethylsiloxane) particles of amphiphilic property. PDMS particles were first obtained using microfluidic devices. A layer of PAA (poly (acrylic acid)) polymer was then selectively grafted on a section of particle surface. After modification, PDMS particles exhibited an amphiphilic property. We demonstrated the assembly of spherical particles at the water/oil interface as well as the deformation of cuboidal particles which possessed a bowl-shaped structure after deformation.

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“…This approach avoids the use of insoluble CaCO 3 powders and ensures the homogeneous distribution of calcium salts in the droplets, and, therefore, provides a reliable method to encapsulate cells in alginate particles. Other microfluidic approaches, such as the fusion of drops containing alginate and cells with drops containing calcium ions to produce Janus alginate particles [96] and to control the shape of alginate particles [97] have also been demonstrated.…”

Section: Synthesis Of Hydrogel Particles Using Microdroplets As Templmentioning

confidence: 99%

Microfluidic-Based Synthesis of Hydrogel Particles for Cell Microencapsulation and Cell-Based Drug Delivery

2012

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Encapsulation of cells in hydrogel particles has been demonstrated as an effective approach to deliver therapeutic agents. The properties of hydrogel particles, such as the chemical composition, size, porosity, and number of cells per particle, affect cellular functions and consequently play important roles for the cell-based drug delivery. Microfluidics has shown unparalleled advantages for the synthesis of polymer particles and been utilized to produce hydrogel particles with a well-defined size, shape and morphology. Most importantly, during the encapsulation process, microfluidics can control the number of cells per particle and the overall encapsulation efficiency. Therefore, microfluidics is becoming the powerful approach for cell microencapsulation and construction of cell-based drug delivery systems. In this article, I summarize and discuss microfluidic approaches that have been developed recently for the synthesis of hydrogel particles and encapsulation of cells. I will start by classifying different types of hydrogel material, including natural biopolymers and synthetic polymers that are used for cell encapsulation, and then focus on the current status and challenges of microfluidic-based approaches. Finally, applications of cell-containing hydrogel particles for cell-based drug delivery, particularly for cancer therapy, are discussed.

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Generation of Janus alginate hydrogel particles with magnetic anisotropy for cell encapsulation

Cited by 112 publications

References 27 publications

A modified microfluidic chip for fabrication of paclitaxel-loaded poly(l-lactic acid) microspheres

A modified microfluidic chip for fabrication of paclitaxel-loaded poly(l-lactic acid) microspheres

A novel method for generation of amphiphilic PDMS particles by selective modification

Microfluidic-Based Synthesis of Hydrogel Particles for Cell Microencapsulation and Cell-Based Drug Delivery

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