Genes and spacers of cloned sea urchin histone DNA analyzed by sequencing

Schaffner, Walter; Kunz, G; Daetwyler, Hans D.; Telford, John L.; Smith, Hamilton O.; Birnstiel, Max L.

doi:10.1016/0092-8674(78)90249-0

Cited by 231 publications

(107 citation statements)

References 34 publications

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“…The only published sea urchin gene sequences are those of the histones, and they do not show introns (21,22). Here we show an intron in a sea urchin actin gene with an exon-intron boundary strikingly similar to that in other organisms.…”

Section: Resultsmentioning

confidence: 65%

The chromosomal arrangement of two linked actin genes in the sea urchin S. purpuratus

Schuler

Keller

1981

Nucl Acids Res

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Four distinct actin genes of the sea urchin Strongylocentrotus purpuratus have been isolated from a recombinant Charon 4 phage library of genomic DNA. The four genes differ considerably from each other in many of their restriction sites. Two of the four genes are closely linked; they are present in the same fragment of cloned DNA. This fragment has been extensively mapped, and some parts of the DNA have been sequenced. The two linked genes are oriented in the same direction, separated by 7.5 kb of DNA. One has an intron following the CAG that codes for the glutamine residue at position 121 in the amino acid sequence of actin. This represents the fifth distinct site at which introns have been found in actin genes, suggesting that the primordial actin gene had at least 6 exons and 5 introns. The actin genes form a distinctive family in which most introns have apparently been precisely excised from the genes.

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Section: Resultsmentioning

confidence: 65%

The chromosomal arrangement of two linked actin genes in the sea urchin S. purpuratus

Schuler

Keller

1981

Nucl Acids Res

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“…These will be denoted as spl (63) (Hentschel et al, 1980). The DNA sequence of the mouse H4 gene in p(mus)-hi-I is known (Seiler-Tuyns and Birnstiel, 1981) as are those of all early histone mRNA genes of both Psammechinus miliaris (Schaffner et al, 1978 and our unpublished results) and Paracentrotus lividus (Mirella Ciaccio, University of Palermo, unpublished results). A common feature of all these genes is that they have no G residue for a considerable distance downstream of the terminal inverted DNA sequence.…”

Section: Resultsmentioning

confidence: 99%

Generation of histone mRNA 3′ ends by endonucleolytic cleavage of the pre-mRNA in a snRNP-dependent in vitro reaction.

Gick¹,

Krämer²,

Keller³

et al. 1986

The EMBO Journal

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Incubation of SP6 generated mouse histone H4 mRNA precursors in nuclear extracts of HeLa cells yields processed mRNA species which end on the 3' adenosine of the conserved terminal ACCA sequence not unlike ten different histone mRNAs isolated from sea urchin embryos which end either on the 3' C or A. In the presence of 20 mM EDTA, the cleaved off 3' spacer portions of the RNA transcripts are readily detected, hence the 3' ends of histone mRNAs arise as a consequence of endonucleolytic cleavage(s) of the precursor RNA. The in vitro cleavage reaction is specifically inhibited by human antisera of the Sm-serotype, but not by control sera and can be rescued by the addition of a preparation of partially purified small nuclear RNPs to the antibody-depleted extract. Interestingly, the snRNP preparation is sufficient to elicit 3' processing of pre-mRNA in the absence of added nuclear extract.

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“…The nucleotide sequence within the coding region reveals little or no homology with the known sequences of histone Hl genes (44) as may be expected from the lack of homology in the N-terminus of the proteins. It is also evident from Table 1 that sea urchin histone mRNA and H5 mRNA have a tendency to use different codons.…”

Section: Agc Cgcggc Ggc Tcc Tcg (C)mentioning

confidence: 99%

Construction of chimeric plasmids containing histone H5 cDNA from hen erythrocyte. DNA sequence of a fragment derived from the 5′ region of H5 mRNA

Ruiz‐Vázquez

Ruiz-Carrillo

1982

Nucl Acids Res

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We report the construction and characterization of chicken erythrocyte histone H5 cDNA recombinant plasmids. cDNA was synthesized from poly(A)+ polysomal RNA enriched in H5 mRNA and inserted into the PseI site of pBR322. Several clones containing H5 cDNA sequences were obtained and one of them (p541), expressing H5 antigenic determinants, was sequenced. The DNA insert of p541 contains 118 nucleotides from the 5' non-translated region of H5 mRNA and sequences coding for up to residue 46 of the N-terminus of the arginine (position 15) H5 variant. There is a strikingly high number of repeated sequences both in the leader and coding region; among these, the octanucleotide 5' GCGGCG GC3' is found five times along the sequence. Although the H5 mRNA 5' leader is GC-rich (66%), there is an AT-rich region, about 16 nucleotides long, which shares strong homology with the leaders of sea urchin histone Hi mRNAs.

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Genes and spacers of cloned sea urchin histone DNA analyzed by sequencing

Cited by 231 publications

References 34 publications

The chromosomal arrangement of two linked actin genes in the sea urchin S. purpuratus

The chromosomal arrangement of two linked actin genes in the sea urchin S. purpuratus

Generation of histone mRNA 3′ ends by endonucleolytic cleavage of the pre-mRNA in a snRNP-dependent in vitro reaction.

Construction of chimeric plasmids containing histone H5 cDNA from hen erythrocyte. DNA sequence of a fragment derived from the 5′ region of H5 mRNA

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