Genetic analysis and mapping of rice (Oryza sativa L.)male-sterile (OsMS-L) mutant

LIUHaisheng,; Chu, Huangwei; Lihui,; WANGHonamei,; WEIJiali,; Lina, Lina; DINGShuyan,; HUANGHai,; MAHong,; HUANGChaofeng,

doi:10.1360/982004-423

Cited by 18 publications

(19 citation statements)

References 17 publications

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“…To clone important genes essential for rice anther development, we generated a rice mutant library using the japonica subspecies 9522 background ( Oryza sativa L. ssp. Japonica ) by treatment with 60 Co γ‐ray (280 Gy) (Liu et al 2005; Wang et al 2006a; Chu et al 2005; Chen et al 2006). Among these mutant lines, one mutant line was complete male sterility (Figure 1A) and displayed normal female development confirmed by reciprocal cross analysis.…”

Section: Resultsmentioning

confidence: 99%

Identification of gamyb‐4 and Analysis of the Regulatory Role of GAMYB in Rice Anther Development

Liu

Bao

Liang

et al. 2010

JIPB

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In higher plants, male reproductive development is a complex biological process that includes cell division and differentiation, cell to cell communication etc., while the mechanism underlying plant male reproductive development remains less understood. GAMYB encodes a gibberellins acid (GA) inducible transcription factor that is required for the early anther development in rice (Oryza sativa L.). Here, we report the isolation and characterization of a new allele gamyb-4 with a C base deletion in the second exon (+2308), causing a frame shift and premature translational termination. Histological analysis showed that gamyb-4 developed abnormal enlarged tapetum and could not undergo normal meiosis. To understand the regulatory role of GAMYB, we carried out quantitative reverse transcription-polymerase chain reaction analysis and comparison of microarray data. These results revealed that the expression of TDR (TAPETUM DEGENERATION RETARDATION), a tapetal cell death regulator, was downregulated in gamyb-4 and udt1 (undeveloped tapetum1). While the GAMYB expression was not obviously changed in tdr and udt1-1, and no apparent expression fold change of UDT1 in tdr and gamyb-4, suggesting that TDR may act downstream of GAMYB and UDT1, and GAMYB and UDT1 work in parallel to regulate rice early anther development. This work is helpful in understanding the regulatory network in rice anther development.

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Section: Resultsmentioning

confidence: 99%

Identification of gamyb‐4 and Analysis of the Regulatory Role of GAMYB in Rice Anther Development

Liu

Bao

Liang

et al. 2010

JIPB

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“…To identify new rice genes regulating male fertility, we generated a rice mutant library using the japonica subspecies 9522 background (O. sativa ssp japonica) by treatment with 60 Co g-ray (280 Gy) and screened for male sterile mutants (Chu et al, 2005;Liu et al, 2005;Chen et al, 2006;Wang et al, 2006). One mutant line displayed complete male sterility and normal female development confirmed by reciprocal cross analysis.…”

Section: Isolation and Phenotypic Analyses Of The Cyp704b2 Mutantmentioning

confidence: 99%

“…v-Hydroxylated C16 and C18:1 fatty acids and fatty alcohols are the essential units for the synthesis of sporopollenin for pollen exine development. C16/C18 fatty acids: palmitic and oleic acids; v-OH C16/C18 fatty acids: 16-hydroxy-palmitic acid and 18-hydroxy-oleic acid; 9(10), v-diOH C16/C18 acids: 9(10),16-dihydroxy-palmitic acid and 9(10),18-dihydroxy-stearic acid; 9,10-epoxy v-OH C18 acid: 9,10-epoxy-18-hydroxy-stearic acid; C16/C18:1 diacids: hexadecane-1,16-dioic acid and octadecene-1,18-dioic acid cross between the cyp704B2 mutant (japonica) and Guan Lu Ai (indica) (Chu et al, 2005;Liu et al, 2005;Chen et al, 2006;Wang et al, 2006). In the F2 population, male sterile plants were selected for gene mapping.…”

Section: Mutant Materials and Growth Conditionsmentioning

confidence: 99%

Cytochrome P450 Family Member CYP704B2 Catalyzes the ω -Hydroxylation of Fatty Acids and Is Required for Anther Cutin Biosynthesis and Pollen Exine Formation in Rice

et al. 2010

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The anther cuticle and microspore exine act as protective barriers for the male gametophyte and pollen grain, but relatively little is known about the mechanisms underlying the biosynthesis of the monomers of which they are composed. We report here the isolation and characterization of a rice (Oryza sativa) male sterile mutant, cyp704B2, which exhibits a swollen sporophytic tapetal layer, aborted pollen grains without detectable exine, and undeveloped anther cuticle. In addition, chemical composition analysis indicated that cutin monomers were hardly detectable in the cyp704B2 anthers. These defects are caused by a mutation in a cytochrome P450 family gene, CYP704B2. The CYP704B2 transcript is specifically detected in the tapetum and the microspore from stage 8 of anther development to stage 10. Heterologous expression of CYP704B2 in yeast demonstrated that CYP704B2 catalyzes the production of omega -hydroxylated fatty acids with 16 and 18 carbon chains. Our results provide insights into the biosynthesis of the two biopolymers sporopollenin and cutin. Specifically, our study indicates that the omega -hydroxylation pathway of fatty acids relying on this ancient CYP704B family, conserved from moss to angiosperms, is essential for the formation of both cuticle and exine during plant male reproductive and spore development.

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“…In this study, six new InDel polymorphic markers were developed for fine-mapping of rl (t) . It is expected that InDel markers will be widely used in mapping and cloning of genes in the near future [29,30] . These markers efficiently fill the gaps of SSR.…”

Section: Development and Application Of Indel Markersmentioning

confidence: 99%

Fine mapping of an incomplete recessive gene for leaf rolling in rice (Oryza sativa L.)

et al. 2005

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Genetic analysis and fine mapping of genes controlling leaf rolling were conducted using two backcrossed generations (BC 4 F 2 , BC 4 F 3 ) derived from a cross between QMX, a non-rolled leaf cultivar as a recurrent parent, and JZB, a rolled leaf NIL of ZB as a donor parent. Results indicated that leaf rolling was mainly controlled by an incompletely recessive major gene, namely rl (t) , and at the same time, affected by quantitative trait loci (QTLs) and/or the environment. A genetic linkage map was constructed using MAPMAKER/EXP3.0 with eight polymorphic markers on chromosome 2, which were screened by BAS method from 500 SSR markers and 15 newly developed insertion/deletion (InDel) markers. The position of rl (t) was estimated with composite interval mapping (CIM) method using WinQTLcart2.5. Gene rl (t) was mapped between markers InDel 112 and RM3763, and 1.0 cM away from InDel 112 using 241 plants in BC 4 F 2 population. To fine map rl (t) , one BC 4 F 3 line with 855 plants was generated from one semi-rolled leaf plant in BC 4 F 2 . Four new polymorphic InDel markers were developed, including InDel 112.6 and InDel 113 located between markers InDel112 and RM3763. Based on the information of recombination offered by 191 rolled leaf plants and 185 non-rolled leaf plants from the BC 4 F 3 line ,we mapped rl (t) to a 137-kb region between markers InDel 112.6 and InDel 113. Homologous gene analysis suggested that rl (t) was probably related to the process of leaf development regulated by microRNA.

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Genetic analysis and mapping of rice (Oryza sativa L.)male-sterile (OsMS-L) mutant

Cited by 18 publications

References 17 publications

Identification of gamyb‐4 and Analysis of the Regulatory Role of GAMYB in Rice Anther Development

Identification of gamyb‐4 and Analysis of the Regulatory Role of GAMYB in Rice Anther Development

Cytochrome P450 Family Member CYP704B2 Catalyzes the ω -Hydroxylation of Fatty Acids and Is Required for Anther Cutin Biosynthesis and Pollen Exine Formation in Rice

Fine mapping of an incomplete recessive gene for leaf rolling in rice (Oryza sativa L.)

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