Genetic analysis of multi-drug resistance and the clonal dissemination of β-lactam resistance in Salmonella Infantis isolated from broilers

Shahada, Francis; Sugiyama, H; Chuma, Takehisa; Sueyoshi, Masuo; Okamoto, Karoku

doi:10.1016/j.vetmic.2009.07.007

Cited by 30 publications

(34 citation statements)

References 29 publications

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“…Different resistance genes have been identified in S. infantis (34). These observations indicate the relationship between the size and the source of class I integrons and efflux proteins encoding resistance genes in this bacteria (28).…”

Section: Discussionmentioning

confidence: 99%

Investigation of Class I Integron in Salmonella infantis and Its Association With Drug Resistance

Asgharpour

Rajabnia

Ferdosi-Shahandashti

et al. 2014

Jundishapur J Microbiol

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Background:Infection with non-typhoid Salmonella (NTS) is one of the most important health problems all over the world. Antimicrobial drug resistance is increasing among Salmonella infantis species.Objectives:The aim of this study was to investigate the frequency of presence of class 1 integrons in S. infantis species as well as its association with drug resistance.Materials and Methods:This cross-sectional study was performed on 50 S. infantis isolated strains, collected from chicken samples between 2009-2011. These strains were identified by standard biochemical tests and serology. Antibiotic susceptibility profiles and minimum inhibitory concentration determination for 14 antibacterial agents were performed using micro dilution and disk diffusion methods. The detection of class 1 integron was performed by the PCR method. The demographic and microbiological data for the integron positive and negative isolates were compared by SPSS software.Results:Eighteen out of 50 (36%) of isolated S. infantis species had intl gene. The isolated bacteria were sensitive to cefotaxime and ciprofloxacin (100%). Also isolates were resistant to nalidixic acid, tetracycline and streptomycin. All isolate with class 1 integron were multidrug resistant.Conclusions:The result of this study showed that due to increased level of drug resistance in S. infantis and the presence of class 1 integron in these strains, resistance can be transferred to other food borne pathogens.

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Section: Discussionmentioning

confidence: 99%

Investigation of Class I Integron in Salmonella infantis and Its Association With Drug Resistance

Asgharpour

Rajabnia

Ferdosi-Shahandashti

et al. 2014

Jundishapur J Microbiol

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“…Recent studies from other countries identified healthy poultry as a potential reservoir of S. enterica ser. Infantis ( 8 – 10 ). …”

Section: Discussionmentioning

confidence: 99%

Multidrug-ResistantSalmonella entericaSerovar Infantis, Israel

Gal-Mor¹,

Valinsky²,

Weinberger³

et al. 2010

Emerg. Infect. Dis.

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“…Detection of resistance genes was performed by polymerase chain reaction (PCR). The amplification reactions were carried out using primers and conditions as previously described (Dahshan et al, 2010; Shahada et al, 2010b). Briefly, the targets were as follows: bla TEM , bla OXA , bla PSE, bla SHV , bla CTX-M-1 group, bla CTX-M-2 group, bla CTX-M-25 , and Toho-1 encoding for penicillinases; bla CIT , bla CMY-2 , bla DHA , bla FOX , bla MOX , bla ACC , and bla EBC encoding for cephalosporinases; tet (A), tet (B), and tet (G) mediating tetracycline efflux proteins; aadA1 and aadA2 encoding for resistance to STR and spectinomycin; sul1 conferring resistance to SUL; and aphA1-Iab encoding for resistance to KAN.…”

Section: Methodsmentioning

confidence: 99%

Chronological Change of Resistance to β-Lactams in Salmonella enterica serovar Infantis Isolated from Broilers in Japan

Chuma¹,

Miyasako²,

Dahshan³

et al. 2013

Front. Microbiol.

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Epidemiologic surveillance study was conducted in southern Japan to determine the antimicrobial resistance phenotypes and characterize the β-lactamase genes and the plasmids harboring these genes in Salmonella enterica serovar Infantis (S. Infantis) isolates from broilers. Between January, 2007 and December, 2008, a total of 1,472 fecal samples were collected and examined at the Laboratory of Veterinary Public Health, Kagoshima University, Japan. In 93 (6.3%) isolates recovered, 33 (35.5%) isolates showed resistance to cefotaxime, an extended-spectrum cephalosporin (ESC), conferred by TEM-20, TEM-52 and CTX-M-25 extended-spectrum β-lactamases (ESBLs). In addition to ESC-resistance, eight (8.6%) isolates exhibited resistance to cefoxitin mediated by CMY-2 AmpC β-lactamase. Plasmid analysis and polymerase chain reaction replicon typing revealed the blaTEM-20 and blaCMY-2 genes were associated with IncP plasmids, blaTEM-52 was linked with a non-typable plasmid and blaCTX-M-25 was carried by an IncA/C plasmid. Non-β-lactam resistance to streptomycin, sulfamethoxazole, and oxytetracycline encoded by the aadA1, sul1, and tet(A) genes, respectively, was found in 86 (92.5%) isolates. Resistance to kanamycin and ofloxacin was exhibited in 12 (12.9%) and 11 (11.8%) isolates, respectively, the former was mediated by aphA1-Iab. These data indicate that S. Infantis isolates producing ESBLs and AmpC β-lactamase have spread among broiler farms in Japan. These data demonstrated that the incidence of ESC-resistant S. Infantis carrying blaTEM-52 remarkably increased and S. Infantis strains harboring blaCMY-2, blaTEM-20, or blaCTX-M-25 genes emerged from broilers in Japan for the first time in 2007 and 2008.

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Genetic analysis of multi-drug resistance and the clonal dissemination of β-lactam resistance in Salmonella Infantis isolated from broilers

Cited by 30 publications

References 29 publications

Investigation of Class I Integron in Salmonella infantis and Its Association With Drug Resistance

Investigation of Class I Integron in Salmonella infantis and Its Association With Drug Resistance

Multidrug-ResistantSalmonella entericaSerovar Infantis, Israel

Chronological Change of Resistance to β-Lactams in Salmonella enterica serovar Infantis Isolated from Broilers in Japan

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