Genetic analysis of resistant to race G of Orobanche cumana Wallr. in F2 and ВС1 of sunflower lines RGP1, RGP2, RGВ, RGL1, RGL2

Guchetl, S.Z.; Antonova, T.S.; Araslanova, N.M.; Chelyustnikova, T.A.; Pitinova, Yu.V.

doi:10.25230/2412-608x-2019-4-180-23-28

Cited by 2 publications

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“…We revealed an incomplete dominance of the resistance gene Or7 to the new virulent broomrape race G during the hybridological analysis carried out at VNIIMK (Guchetl et al, 2019). Moreover, we continue the search for molecular genetic markers loci that control broomrape resistance.…”

Section: Breeding For Disease Resistancementioning

confidence: 80%

Modern trends in breeding and genetic improvement of sunflower varieties and hybrids at VNIIMK

2021

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Modern sunflower breeding is significantly diversified due to the different needs of agricultural production. The breeding of sunflower varieties and hybrids is carried out at V.S. Pustovoit All-Russian Research Institute of Oil Crops (VNIIMK) in all areas in demand on the market and is based on fundamental biological research. In the field of breeding for faster maturing, the following commercial cultivars were obtained: very early maturing, cv. Skormas and the three-way hybrid Achilles, early maturing cvs. Varyag and Uspekh, medium maturing cvs. Amelie, Aris and Aurus. Within the framework of breeding for immunity, eight hybrids and one variety have been produced. So at the Don experimental station (Rostov region), productive hybrids were bred, resistant to the virulent broomrape of the G race due to the presence of the Or7 gene: ‘Gorstar’, ‘Gorfild’, ‘Grant’, ‘Status’, ‘Fogor’ and the three-way hybrid Nika. On the central experimental base, the following were obtained: the mid-early hybrid Typhoon and the earlymaturing variety Platonych with resistance to common races of downy mildew and a high oil content of achenes (up to 53 %) as well as the mid-early hybrid Tayzar, which is simultaneously resistant to virulent races of broomrape and to the causative agent of downy mildew. The early maturing large-fruited sunflower variety Belochka was included in the“Russian State Register of Selection Achievements…”, and the large-fruited varieties Karavan, Konditer and Kalibr are currently undergoing state tests. The breeding use of germplasm with genes for herbicide resistance wasaccompanied by their extensive genetic study. A practical recommendation for all three alleles of the ALS gene (Imr, CLHA-Plus, Sur) was the need to create homozygous hybrids for their reliable use in appropriate production systems. For Clearfield technology, the hybrids Imidzh, Arimi and Immi have been developed; for Clearfield Plus, the hybrid Klip; and for Express Sun (or SUMO), the hybrid Surus. Klip and Surus are mid-oleic. All newly developed fertile ornamental sunflower varieties – Aurelia, Fizalia, Zhemchuzhny, Rumyanets, Agat and Mazhor – were transferred for practical use to a sterile CMS RIG basis. Thus, new achievements have been attained across the entire spectrum of modern trends in sunflower breeding.

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Section: Breeding For Disease Resistancementioning

confidence: 80%

Modern trends in breeding and genetic improvement of sunflower varieties and hybrids at VNIIMK

2021

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The search for the location of the sunflower resistance gene to race G of broomrape on the linkage map in the lines of breeding of VNIIMK

Guchetl

Savichenko

Рамазанова

2021

IOP Conf. Ser.: Earth Environ. Sci.

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The parasitic plant Orobanche cumana Wallr. is one of the main biotic limiting factors for obtaining high yields of sunflower. Since plant resistance to different broomrape races in different genotypes can be controlled by different genes, to ensure the evaluation of breeding material using DNA markers (MAS), it is necessary to localize the resistance gene for each of the sources. The aim of the research is to find the location of the Or7 gene, which controls the resistance to one of the most virulent broomrape races, on the sunflower linkage map and to determine the DNA markers cosegregating with it. For research we used the lines of breeding of V.S. Pustovoit of All-Russian Research Institute of Oil Crops (VNIIMK) resistant and susceptible to race G of broomrape. We used SSR-primers ORS 683, ORS 1040, ORS 1112, and ORS 202 for the PCR analysis. We excluded the location of the gene in the upper part of LG3 in the immediate proximity to the microsatellite loci ORS 683, ORS 1040, ORS 1112. Based on the studied literary sources and the reference genome of the sunflower HanXRQr2.0-SUNRISE, we compiled a partial physical map of LG3 and determined the area for further search for the localization of the Or7 gene and DNA markers cosegregating with it.

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Genetic analysis of resistant to race G of Orobanche cumana Wallr. in F2 and ВС1 of sunflower lines RGP1, RGP2, RGВ, RGL1, RGL2

Cited by 2 publications

References 0 publications

Modern trends in breeding and genetic improvement of sunflower varieties and hybrids at VNIIMK

Modern trends in breeding and genetic improvement of sunflower varieties and hybrids at VNIIMK

The search for the location of the sunflower resistance gene to race G of broomrape on the linkage map in the lines of breeding of VNIIMK

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