Genetic assessment of the internal transcribed spacer region (ITS1.2) in Mangifera indica L. landraces

Abstract

Mango (Mangifera indica) is one of the most important tropical fruits in the world. Twenty-two genotypes of native mangoes from different regions of southern Iran (Hormozgan and Kerman) were collected and analyzed for the ribosomal genes. GC content was found to be 55.5%. Fu and Li's D* test statistic (0.437), Fu and Li's F* test statistic (0.500) and Tajima's D (1.801) were positive and nonsignificant. A total of 769 positions were identified (319 with insertion or deletion including 250 polymorphic and 69 monomorphic loci; 450 loci without any insertion or deletion including 35 Singletons and 22 haplotypes). Nucleotide diversity of 0.309 and a high genetic differentiation including Chi square of 79.8; P value of 0.3605 and df value of 76 was observed among mango genotypes studied. The numerical value of the ratio dN/dS (0.45) indicated a pure selection in the examined gene and the absence of any key changes. Cluster analysis differentiated the mango used in this research (M. indica L.) into two genotypes but could not differentiate their geographical locations. The results of this study indicated that a high genetic distance exists between HajiGholam (Manojan) and Arbabi (Rodan) genotypes and showed higher genetic diversity in mango of Rodan region. Results of present study suggested that for successful breeding, the genotypes of Rodan region mango especially Arbabi mango can be used as a gene donor and ITS can be a suitable tool for genetic evaluations of inter and intra species.

Evaluation of Genetic Variations of Mangifera sp. in Southern Vietnam

“…This result was similar to Hidayat et al 9 when separating mango varieties collected from different countries Malaysia, India, Indonesia, Thailand, Taiwan into 3 different groups based on ITS region. The ITS sequence also exhibited high diversity in DNA sequence characteristics and can be effectively applied in the molecular identification of native mangoes in Southern Iran 26 . Even with high diversity, we found that the genetic similarity of intra-genetic samples based on ITS was not affected by the geographical sampling sites.…”

Dissimilarity in the Nuclear Ribosomal DNA Internal Transcribed Spacer Regions of Haplophyllum spp. Founded in Ashdagh Mountain, Sangaw, Kurdistan of Iraq

“…is sister to subclade of H. tuberculatum and H. blanchei. ITS 1 and ITS2 are widely used in plant phylogenetic studies due to their ability to teasing out the intra-and interspecific variation among species [10]. In general, the base substitutions in the ITS region among Haplophyllum species are more than other species in angiosperm [2].…”

“…However, few species have been identified or studied through molecular approaches [2]. Nuclear ribosomal internal transcribed spacer (ITS) regions are more powerful than other plastid regions in identification of intraspecific variability among species of the same genus [10][11][12]. ITS regions are characterized as ITS1 and ITS2.…”

Application of different molecular markers in biotechnology

“…ITS is a degraded region during maturation in ribosomal transcript. (Choudhary et al, 2015) ITS due to more variation properties than ribosomal sequences have been commonly used for molecular markers to figure out phylogenetic analysis and/or identification of bacteria, plant and animal strains and/or species (Lee et al, 2017;Rocha et al, 2019;Ali et al, 2019;Fazeli-Nasab et al, 2020). Pourahmad et al (2019) performed sequence analysis of the mycobacterial 16S-23S ITS region to determine aquatic mycobacteria species, reporting effectiveness of this marker.…”