Genetic Assessment of Traits and Genetic Relationship in Blackgram (Vigna mungo) Revealed by Isoenzymes

Singh, Ajay Kumar; Mishra, Avinash; Shukla, Arvind Kumar

doi:10.1007/s10528-009-9247-1

Cited by 4 publications

(4 citation statements)

References 26 publications

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“…The radical length had positive and significant correlation with dry matter weight at 16.0 EC level and positive and non significant correlation with higher EC level suggested that radicle length is the most useful parameter to evaluate salt tolerant cultivars of crop plants. Similar finding was also reported by Singh et al, (2009);Kuhad and Sheoran;(1987); Freitas and Camargo, (1988) ;Shekhar, (1994). Positive correlation of germination and radicle length at higher salt concentrations Screening of Black Gram (Vigna Mungo L. Hepper) Varieties for Tolerance to Salinity was affected more due to increasing salt concentration followed by VBG-12-034.…”

Section: Simple Linear Correlation Coefficient Among 5 Characters In supporting

confidence: 89%

“…The experimental material comprised of eight popular varieties (VBN1, VBN2, VBN3, VBN(Bg)4, VBN(Bg)5, VBN(Bg)6, VBN 7 and VBN 8) and of five advanced pipeline cultures , VBG 14-016) of black gram collected form National Pulses Research Centre, Vamban and the experiment was conducted at Agricultural College and Research Institute, Kudumiyanmalai during the year 2018-19 its Latitude is 10.38 North and Longitude is 78.82. Thirteen genotypes were screened using a combination of salts viz., NaCl, Na 2 SO 4 , KCl, K 2 SO 4 , MgCl 2 , MgSO 4 and CaCl 2 in the ratio of 2:1:1:1:1:1:1, respectively dissolved in deionized water to of 100 ml solution (Singh et al 2009), to create EC of 4.0 ds/m level 0.075 g of NaCl and 0.037 g for the remaining salts dissolved in the 100 ml of deionised water. Similarly for 11.0 ds/m EC, 0.175 g of NaCl and 0.0875 g of other salts were dissolved in the 100 ml of deionised water, 16.0 ds/m EC was achieved by dissolving 0.25 g of NaCl and 0.125 g of remaining salts were dissolved in the 100 ml of deionised water and deionised water is used as control (0.00 EC) with no salts.…”

Section: Methodsmentioning

confidence: 99%

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Screening of Black Gram (Vigna Mungo L. Hepper) Varieties for Tolerance to Salinity

Shanthi

Ramesh

Sakaravarthy

et al. 2019

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Salinity is one of the most important abiotic stress that affects the yield in most of the crops under cultivation. The area under black gram (Vigna mungo L. Hepper) is decreasing in recent years due to soil salinity problem. Thirteen black gram genotypes viz., VBN1, VBN2, VBN3, VBN(Bg) 4, VBN(Bg ) 5, VBN(Bg) 6, VBN 7 and VBN 8, VBG 12-034, VBG 12-062, VBG 12-110, VBG 12-111, VBG 13-003 and VBG 14-016 were screened under three EC level (4.0 EC, 11.0 EC and 16.0 EC) and compared with 0.0 EC (control). The mean germination percentage of all the thirteen genotypes studied illustrated reduced level of germination percentage with increasing salinity level. At the highest salinity level (16.0 EC) the germination percentage was significantly affected compared to 4.0 EC and 11.0 EC. The grand mean of plumule length was more at 4.0 EC and was reduced to half (16.0 EC) as compared to control. The root grew longer at 11.0 EC (4.91 cm) as compared to 4.0 EC (4.83 cm) and 0.0 EC (3.02 cm), where ever showed drastic reduction at 16.0 EC (1.92 cm). The grand mean value of dry matter weight increased concomitantly with salinity. The radical length had positive and significant correlation with dry matter weight at 11.0 EC (0.657) where as positive and non-significant correlation with 4.0 and 16.0 EC suggested that radicle length is the most useful parameter to select salinity tolerant black gram genotypes. Based on grand mean performance of the thirteen genotypes evaluated, the genotype VBG-14-016 followed by Vamban 4, Vamban 8 and VBG-12-062 outperformed than all other genotypes, while VBG-13-003 was highly susceptible followed by VBG-12-034.

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Section: Simple Linear Correlation Coefficient Among 5 Characters In supporting

confidence: 89%

Section: Methodsmentioning

confidence: 99%

Screening of Black Gram (Vigna Mungo L. Hepper) Varieties for Tolerance to Salinity

Shanthi

Ramesh

Sakaravarthy

et al. 2019

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“…Compared to cowpea and mungbean, there has been less research on black gram, especially in terms of molecular genetic diversity. Genetic variability of black gram has been studied based on morphological and agronomic traits ( Ghafoor et al 2001 , Gupta et al 2001 ), seed storage protein ( Ghafoor and Ahmad 2005 ), isozyme markers ( Singh et al 2009 ), DNA markers including random amplified polymorphic DNA (RAPD), inter simple sequence repeat (ISSR), amplified fragment length polymorphism (AFLP), and simple sequence repeat (SSR) ( Gupta and Gopalakrishna 2009 , Sivaprakash et al 2004 , Souframanien and Gopalakrishna 2004 ). However, these studies have provided little information on the extent of genetic diversity in black gram because each study, except Gupta et al (2001) and Ghafoor et al (2001) , employed less than 150 accessions, and the germplasm used in each study originated from only a single geographical region (country).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of the black gram [<i>Vigna mungo</i> (L.) Hepper] gene pool as revealed by SSR markers

et al. 2015

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In this study, 520 cultivated and 14 wild accessions of black gram (Vigna mungo (L.) Hepper) were assessed for diversity using 22 SSR markers. Totally, 199 alleles were detected with a mean of 9.05 alleles per locus. Wild black gram showed higher gene diversity than cultivated black gram. Gene diversity of cultivated accessions among regions was comparable, while allelic richness of South Asia was higher than that of other regions. 78.67% of the wild gene diversity presented in cultivated accessions, indicating that the domestication bottleneck effect in black gram is relatively low. Genetic distance analysis revealed that cultivated black gram was more closely related to wild black gram from South Asia than that from Southeast Asia. STRUCTURE, principal coordinate and neighbor-joining analyses consistently revealed that 534 black gram accessions were grouped into three major subpopulations. The analyses also revealed that cultivated black gram from South Asia was genetically distinct from that from West Asia. Comparison by SSR analysis with other closely related Vigna species, including mungbean, azuki bean, and rice bean, revealed that level of gene diversity of black gram is comparable to that of mungbean and rice bean but lower than that of azuki bean.

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“…Four levels of salinity (4.0 EC, 8.0 EC, 12.0 EC and 16.0 EC dsm -1 ) were compared with control (0.0 EC). Since salinity is not due to the single salt stress the experiment solution with different EC levels were prepared using combination of salts viz., NaCl, Na 2 SO 4 , KCl, K 2 SO 4 , MgCl 2 , MgSO 4 and CaCl 2 in the ratio of 2:1:1:1:1:1:1, respectively dissolved in deionized water (Singh et al, 2009;Shanthi et al, 2021). Screening was done by roll towel method with two replications and 20 seeds per replication were placed for germination in each treatment.…”

Section: Methodsmentioning

confidence: 99%

Assessment of Salinity Tolerance in Greengram [Vigna radiata (L.) Wilczek]

Shanthi¹,

Parameshwaran²,

Umadevi³

et al. 2023

ASD

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Background: Greengram is one of the important and salt sensitive food legume crops with high nutritional quality. The area under salinity is increasing gradually due to various reasons like global warming, continues irrigation of bore well water and use of more organic fertilizers etc. Identification of salinity tolerant genotype in greengram is highly essential to improve the production and productivity. Salinity stress condition increases the absorption of more Na+ whereas the reduction of K+ absorption was noticed, which thus decreases the K+ /Na+ ratio. The first visible symptom due to salinity stress was retarded growth because of more uptake of Na+ ions leads to reduction of physiological activities. The next important character affected by salinity stress was radical growth. Methods: A total of 20 greengram genotypes were evaluated for salinity tolerance under four EC levels. Screening was done by roll towel method with two replications. The roll towels were placed in different buckets filled with different EC level of saline water and observations were recorded on the 8th day of germination. Result: The salinity level of more than 4.0 EC dsm-1 causes gradual reduction in radicle length and at 12.0 EC level almost in all the genotypes the radicle length was reduced to half as compare to control. The correlation analysis indicated that the plumule length and radicle length were the important criteria for selection to salinity tolerance. While observing the other growth parameters like plumule and radical length reduction, dry matter weight and salinity tolerance and susceptible index the genotypes IPMD 14-10 and VGG 17-038 were identified as more tolerant genotypes and DGGV 80, PUSA-BM5 and VBN 4 were more sensitive genotypes.

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Genetic Assessment of Traits and Genetic Relationship in Blackgram (Vigna mungo) Revealed by Isoenzymes

Cited by 4 publications

References 26 publications

Screening of Black Gram (Vigna Mungo L. Hepper) Varieties for Tolerance to Salinity

Screening of Black Gram (Vigna Mungo L. Hepper) Varieties for Tolerance to Salinity

Genetic diversity of the black gram [<i>Vigna mungo</i> (L.) Hepper] gene pool as revealed by SSR markers

Assessment of Salinity Tolerance in Greengram [Vigna radiata (L.) Wilczek]

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