Genetic Basis of Multidrug Resistance in
            <i>Acinetobacter</i>
            Clinical Isolates in Taiwan

Lin, Yu-Chi; Hsia, Ko-Chiang; Chen, Yee‐Chun; Sheng, Wang-Huei; Chang, Shan-Chwen; Liao, Ming‐Feng; Li, Shuying

doi:10.1128/aac.01398-09

Cited by 46 publications

(33 citation statements)

References 40 publications

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“…This was unlikely, as transformation of a plasmid harboring only the ISAba1-bla OXA-51 -like gene (ISAba1-bla OXA-82 ) was enough to confer a high level of carbapenem resistance. This phenomenon has also been recently demonstrated (16). Interestingly, the N-type isolates also had a higher sulbactam resistance rate, the mechanism of which is undetermined.…”

Section: Discussionsupporting

confidence: 63%

“…For example, ISAba1 upstream of the bla OXA-51 -like gene is associated with the overexpression of the bla OXA-51 -like gene and carbapenem resistance in A. baumannii (12,23). However, some isolates harboring the bla OXA-51 -like gene with an upstream ISAba1 (ISAba1-bla OXA-51 -like gene) are still susceptible to carbapenem (9,14,16). The reason for this discrepancy in carbapenem susceptibility levels in isolates harboring the ISAba1-bla OXA-51 -like gene has not been completely clarified.…”

mentioning

confidence: 99%

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Emergence and Distribution of Plasmids Bearing the bla _OXA-51 -Like Gene with an Upstream IS Aba1 in Carbapenem-Resistant Acinetobacter baumannii Isolates in Taiwan

Chen

Lee

Kuo

et al. 2010

Antimicrob Agents Chemother

117

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The bla OXA-51 -like gene with an upstream ISAba1 (ISAba1-bla OXA-51 -like gene) was originally found on the chromosomes of carbapenem-resistant or -susceptible Acinetobacter baumannii isolates. However, a plasmidborne ISAba1-bla OXA-51 -like gene has recently been identified in Acinetobacter genomic species 13TU and several A. baumannii isolates in Taiwan, and all of the isolates are carbapenem resistant. This study aimed to characterize the plasmids bearing the ISAba1-bla OXA-51 -like gene and their significance in A. baumannii. Among the 117 ISAba1-bla OXA-51 -like-harboring isolates collected from 10 hospitals in Taiwan, 58 isolates (49.6%) from 24 clones had the genes located on plasmids that likely originated from a common progenitor. Among the 58 isolates, four had additional copy of the ISAba1-bla OXA-51 -like gene on their chromosomes. Based on the analysis of these four isolates, the plasmid-located ISAba1-bla OXA-51 -like gene appeared to be acquired via one-ended transposition (Tn6080). The isolates with a plasmid bearing the ISAba1-bla OXA-51 -like gene had higher rates of resistance to imipenem (98% versus 46.6%; P < 0.001) and meropenem (98% versus 69%; P ‫؍‬ 0.019) than those with the genes chromosomally encoded, which is most likely due to increased gene dosage provided by the higher copy number of associated plasmids. Transformation with a recombinant plasmid harboring only the ISAba1-bla OXA-51 -like gene was enough to confer a high level of carbapenem resistance to A. baumannii, eliminating the possible contribution of other factors on the original plasmids. This study demonstrated that the carbapenem resistance-associated plasmids carrying the ISAba1-bla OXA-51 -like gene are widespread in A. baumannii strains in Taiwan.

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Section: Discussionsupporting

confidence: 63%

mentioning

confidence: 99%

Emergence and Distribution of Plasmids Bearing the bla _OXA-51 -Like Gene with an Upstream IS Aba1 in Carbapenem-Resistant Acinetobacter baumannii Isolates in Taiwan

Chen

Lee

Kuo

et al. 2010

Antimicrob Agents Chemother

117

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“…However, the distribution of gene cassettes in our study differed from that found in food samples in other countries such as Tunisia and Norway, where dfrA1-aadA1 was the dominant array [17,18]. The cassette array aacA4-catB8-aadA1, encoding tobramycin, chloramphenicol, and streptomycin resistance, respectively, has been found frequently in Acinetobacter isolates in many previous studies [19,20,21]. To our knowledge, aacA4-catB8-aadA1 was not common among E. coli isolates [22,23].…”

Section: Discussioncontrasting

confidence: 88%

Characterization and horizontal transfer of class 1 integrons in Escherichia coli isolates from cooked meat products

Zhang

Jiang

et al. 2016

J Infect Dev Ctries

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Introduction: Escherichia coli is a commensal bacterium in humans, animals, and the environment that is one of the microorganisms commonly resistant to antimicrobials. Cooked meat products, which are popular in China, are easily contaminated by E. coli during processing and storage. Methodology: In this study, a total of 75 E. coli isolates from cooked meat products in Henan province, China, were assayed for the presence of and horizontal transfer of class 1 integrons. Results: Class 1 integrons were detected in 11 (14.7%) of these isolates, and contained four groups of resistance gene cassettes, including dfrA17-aadA5, dfrA1-aadA1, dfrA12-orfF-aadA2, and an uncommon array of aacA4-catB8-aadA1. The transfer frequency of selected integron-positve donors ranged from 10 -6 to 10 -4 transconjugants per recipient cell, and the integron-containing DNA from the donors could be transferred to E. coli J53Az r with the transformation frequency of 10 -7 to 10 -5 . Conclusions: Class 1 integrons could be transferred to recipient E. coli J53 by conjugation and natural transformation. These findings suggest the role of commensal E. coli isolates from cooked meats as an important reservoir for integrons and the possible transfer of antimicrobial resistance genes to humans via the food chain.

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“…These results are similar to those in our report. However, a report from another medical center in northern Taiwan revealed that only 3.4% of CRAB isolates harbored the bla OXA-23 -like gene (11). Reports from 2 hospitals in central Taiwan showed a higher occurrence of the bla OXA-23 -like gene (93% and 96%, respectively) (12,13).…”

Section: Discussionmentioning

confidence: 98%

Types and Prevalence of Carbapenem-Resistant Acinetobacter calcoaceticus-Acinetobacter baumannii Complex in Northern Taiwan

Hsieh

Wang

Feng

et al. 2014

Antimicrob Agents Chemother

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eThe frequency of the carbapenem-resistant Acinetobacter calcoaceticus-Acinetobacter baumannii (CRACB) complex increases annually in our hospitals. However, the types and prevalence of carbapenemases among isolates still remain unclear. In this study, we identified and collected 672 carbapenem-resistant isolates from a medical center in Northern Taiwan between April and December of 2010. There were 577 genospecies 2 (Acinetobacter baumannii), 79 genospecies 13TU, and 16 genospecies 3 isolates. The isolates had an acquired bla OXA-24 -like gene, which was confirmed by sequencing for the encoded OXA-72 carbapenemase, and were often associated with high-level carbapenem resistance. These CRACB complex isolates remained susceptible to colistin (100%). The genotyping of isolates was conducted using pulsed-field gel electrophoresis with ApaI digestion. In most clonally related groups, patients were from both branch hospitals. The results indicate that interhospital dissemination of clones occurred. This study provides updated data on the types and prevalence of the CRACB complex. In addition, it presents a warning on the emergence and spread of CRACB complex harboring bla OXA-24 -like genes in northern Taiwan.

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Genetic Basis of Multidrug Resistance in Acinetobacter Clinical Isolates in Taiwan

Abstract: Multidrug-resistant (MDR)Acinetobacter

Cited by 46 publications

References 40 publications

Emergence and Distribution of Plasmids Bearing the bla _OXA-51 -Like Gene with an Upstream IS Aba1 in Carbapenem-Resistant Acinetobacter baumannii Isolates in Taiwan

Emergence and Distribution of Plasmids Bearing the bla _OXA-51 -Like Gene with an Upstream IS Aba1 in Carbapenem-Resistant Acinetobacter baumannii Isolates in Taiwan

Characterization and horizontal transfer of class 1 integrons in Escherichia coli isolates from cooked meat products

Types and Prevalence of Carbapenem-Resistant Acinetobacter calcoaceticus-Acinetobacter baumannii Complex in Northern Taiwan

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Genetic Basis of Multidrug Resistance in Acinetobacter Clinical Isolates in Taiwan

Abstract: Multidrug-resistant (MDR)Acinetobacter

Cited by 46 publications

References 40 publications

Emergence and Distribution of Plasmids Bearing the bla OXA-51 -Like Gene with an Upstream IS Aba1 in Carbapenem-Resistant Acinetobacter baumannii Isolates in Taiwan

Emergence and Distribution of Plasmids Bearing the bla OXA-51 -Like Gene with an Upstream IS Aba1 in Carbapenem-Resistant Acinetobacter baumannii Isolates in Taiwan

Characterization and horizontal transfer of class 1 integrons in Escherichia coli isolates from cooked meat products

Types and Prevalence of Carbapenem-Resistant Acinetobacter calcoaceticus-Acinetobacter baumannii Complex in Northern Taiwan

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Emergence and Distribution of Plasmids Bearing the bla _OXA-51 -Like Gene with an Upstream IS Aba1 in Carbapenem-Resistant Acinetobacter baumannii Isolates in Taiwan

Emergence and Distribution of Plasmids Bearing the bla _OXA-51 -Like Gene with an Upstream IS Aba1 in Carbapenem-Resistant Acinetobacter baumannii Isolates in Taiwan