Genetic characterization of influenza A virus subtype H12N1 isolated from a watercock and lesser whistling ducks in Thailand

Wongphatcharachai, Manoosak; Wisedchanwet, Trong; Lapkuntod, Jiradej; Nonthabenjawan, Nutthawan; Jairak, Waleemas; Amonsin, Alongkorn

doi:10.1007/s00705-012-1260-8

Cited by 14 publications

(8 citation statements)

References 24 publications

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“…Recently, a small number of H4, H6 and H10 viruses were isolated from healthy Muscovy ducks at a central Bangkok live bird market (Wisedchanwet et al, 2011). Additionally, H12N1 viruses were isolated from wild ducks and a watercock in Central Thailand, both species which may frequent flooded rice fields (Wongphatcharachai et al, 2012). Other avian species known to feed at Thai rice fields, such as the little egret, Eurasian tree sparrow, scaly-breasted munia, common myna, white-vented myna, Asian open-bill stork, heron and dove, have been identified with confirmed HPAI H5N1 infection (Dierauf et al, 2006;Siengsanan et al, 2009).…”

Section: Influenza Virusesmentioning

confidence: 99%

Risk Factors for Exposure to Influenza A Viruses, Including Subtype H5 Viruses, in Thai Free-Grazing Ducks

Beaudoin

Kitikoon

Schreiner

et al. 2012

Transbound Emerg Dis

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Free-grazing ducks (FGD) have been associated with highly pathogenic avian influenza (HPAI) H5N1 outbreaks and may be a viral reservoir. In July-August 2010, we assessed influenza exposure of Thai FGD and risk factors thereof. Serum from 6254 ducks was analysed with enzyme-linked immunosorbent assay (ELISA) to detect antibodies to influenza A nucleoprotein (NP), and haemagglutinin H5 protein. Eighty-five per cent (5305 ducks) were seropositive for influenza A. Of the NP-seropositive sera tested with H5 assays (n = 1423), 553 (39%) were H5 ELISA positive and 57 (4%) suspect. Twelve per cent (74 of 610) of H5 ELISA-positive/suspect ducks had H5 titres ≥ 1 : 20 by haemagglutination inhibition. Risk factors for influenza A seropositivity include older age, poultry contact, flock visitors and older purchase age. Study flocks had H5 virus exposure as recently as March 2010, but no HPAI H5N1 outbreaks have been identified in Thailand since 2008, highlighting a need for rigorous FGD surveillance.

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Section: Influenza Virusesmentioning

confidence: 99%

Risk Factors for Exposure to Influenza A Viruses, Including Subtype H5 Viruses, in Thai Free-Grazing Ducks

Beaudoin

Kitikoon

Schreiner

et al. 2012

Transbound Emerg Dis

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“…Secondly, there was a lack of local avian influenza virus strains used in the serological tests. At present, various LPAI subtypes, including H1, H3, H4, H7, H8, H10, and H11, have been identified in domestic avian species [ 23 , 24 , 28 , 29 , 30 , 31 ] and the LPAI subtype of H12 has been successfully isolated from a watercock and lesser whistling duck in Thailand [ 32 ]. Based on our virus resources, we used 16 influenza HA subtypes (H1–H16) of the AIV and two Thailand strains of the human H1N1 influenza virus as the tested antigen.…”

Section: Discussionmentioning

confidence: 99%

Evidence of Influenza A Virus Infection in Cynomolgus Macaques, Thailand

Paungpin

Thongdee

Ketchim

et al. 2022

Veterinary Sciences

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Little is known about the ecology of influenza A virus (IAV) in nonhuman primates (NHPs). We conducted active surveillance of IAV among 672 cynomolgus macaques (Macaca fascicularis) living in 27 free-ranging colonies in Thailand between March and November 2019. A hemagglutination inhibition (HI) assay was employed as the screening test against 16 subtypes of avian influenza virus (AIV) and two strains of the H1 subtype of human influenza virus. The serum samples with HI titers ≥20 were further confirmed by microneutralization (MN) assay. Real-time RT-PCR assay was performed to detect the conserved region of the influenza matrix (M) gene. The seropositive rate for subtypes of IAV, including AIV H1 (1.6%, 11/672), AIV H2 (15.2%, 102/672), AIV H3 (0.3%, 2/672), AIV H9 (3.4%, 23/672), and human H1 (NP-045) (0.9%, 6/672), was demonstrated. We also found antibody against more than one subtype of IAV in 15 out of 128 positive tested sera (11.7%). Moreover, influenza genome could be detected in 1 out of 245 pool swab samples (0.41%). Evidence of IAV infection presented here emphasizes the role of NHPs in the ecology of the virus. Our findings highlight the need to further conduct a continuous active surveillance program in NHP populations.

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“…For example, there was evidence that identical LPAI viruses could be isolated from both domestic ducks and wild birds in China. In Thailand, IAV subtypes H4N6, H4N9, H7N6, and H10N3 have been reported in Muscovy ducks housing in live bird markets 34,35 . Additionally, other LPAI viruses, IAV‐H12N1, were isolated from watercock and lesser‐whistling ducks 35 .…”

Section: Discussionmentioning

confidence: 99%

“…In Thailand, IAV subtypes H4N6, H4N9, H7N6, and H10N3 have been reported in Muscovy ducks housing in live bird markets 34,35 . Additionally, other LPAI viruses, IAV‐H12N1, were isolated from watercock and lesser‐whistling ducks 35 . There are also several reports of IAVs circulating in ducks in Asia, for example, IAV‐H5N6 in Vietnam, IAV‐H7N4 in Cambodia, and IAV‐H5N3 in waterfowl and domestic ducks in China 36,37 .…”

Section: Discussionmentioning

confidence: 99%

Genetic characterization of influenza A virus subtypes H11N6, H11N7, and H11N9 isolated from free‐grazing ducks, Thailand

Chaiyawong

Charoenkul

Udom

et al. 2022

Influenza Resp Viruses

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Influenza A viruses (IAVs) infect avian species and several mammalian species including humans. Anseriformes water birds are an important reservoir of IAVs. In this study, we identified and characterized IAV subtypes H11N6 (n = 5), H11N7 (n = 3), and H11N9 (n = 3) isolated during the influenza surveillance program in free‐grazing ducks from 2012 to 2015 in Thailand. Eleven IAV‐H11 viruses were characterized by either whole genome sequencing (n = 5) or HA and NA gene sequencing (n = 6) for phylogenetic and amino acid analyses. Phylogenetic analysis showed that Thai IAV‐H11 were grouped into Avian Eurasian lineage. Amino acid analysis showed that all Thai IAV‐H11 viruses have low pathogenic avian influenza (LPAI) characteristics and sensitive to Oseltamivir and Amantadine. Novel reassortant viruses (IAV‐H11N7 and IAV‐H11N9) have been observed. The reassortant viruses contained NP, M, and NS gene segments which originate from intercontinental sources which never been reported in Thai IAVs. In summary, this study demonstrated high genetic diversity of IAV‐H11 circulating in free‐grazing ducks. Free‐grazing ducks infected with IAVs generated novel reassortant IAV‐H11. Thus, surveillance of IAVs in free‐grazing ducks should be routinely conducted to monitor novel reassortant viruses and subsequently potential virulence viruses.

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Genetic characterization of influenza A virus subtype H12N1 isolated from a watercock and lesser whistling ducks in Thailand

Cited by 14 publications

References 24 publications

Risk Factors for Exposure to Influenza A Viruses, Including Subtype H5 Viruses, in Thai Free-Grazing Ducks

Risk Factors for Exposure to Influenza A Viruses, Including Subtype H5 Viruses, in Thai Free-Grazing Ducks

Evidence of Influenza A Virus Infection in Cynomolgus Macaques, Thailand

Genetic characterization of influenza A virus subtypes H11N6, H11N7, and H11N9 isolated from free‐grazing ducks, Thailand

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