Genetic diversity among ‘Candidatus Liberibacter asiaticus’ isolates based on single nucleotide polymorphisms in 16S rRNA and ribosomal protein genes

Asian-common strains of ‘Candidatus Liberibacter asiaticus’ are distributed in Northeast India, Papua New Guinea and Timor-Leste

“…Accession numbers obtained for the phylogenetic analysis are listed in Table 2. The alignment showed no genetic diversity among Japanese, Southeast Asian, Oceanian, American (Florida), and Brazilian isolates, as reported previously (Adkar-Purushothama et al 2009;Tomimura et al 2009). All Northeast Indian isolates were identical to the Asian/Oceanian/American isolates.…”

“…In many Japanese isolates and several Southeast Asian isolates, including those from Taiwan, Indonesia, the Philippines, Vietnam, and Thailand, the 16S rDNA sequences are identical (Subandiyah et al 2000;Tomimura et al 2009). However, numerous small nucleotide polymorphisms (SNPs) have been reported in many Chinese isolates and in two Indian isolates collected from Karnataka, a state in Southwest India (Adkar-Purushothama et al 2009). The presence of 'Ca.…”

“…Therefore, the sequence diversity among the Asian 'Ca. L. asiaticus' isolates could not be compared precisely, apparently resulting in the erroneous establishment of an independent 16Sr group Abbreviations are the same as in Table 2; sequences for accessions in boldface were determined in this study (Adkar-Purushothama et al 2009). Our results also indicate that the Poona strain is less suitable than other Asiancommon isolates as a genetic reference for 'Ca.…”

“…2a, b). When classified according to the 16S rDNA SNP lineage by Adkar-Purushothama et al (2009), the Northeast Indian isolates belong to group 16Sr-VI, which consists of Japanese, Southeast Asian (Taiwan and Malaysia), American (Florida), and Brazilian isolates, while the other Indian isolates separated into a different group (16Sr-I, 16Sr-II, and 16Sr-X).…”

First report of ‘Candidatus Liberibacter asiaticus’ associated with Citrus decumana (Beejapuraka) in South India

“…It is used as antioxidant, anti-inflammatory and analgesic agent (Sood et al 2009). Citrus huanglongbing (HLB, ex greening) is one of the most serious, destructive and widespread diseases of citrus cultivars in Asia, Africa and American continents (Adkar-Purushothama et al 2009). It is associated with three uncultured phloem-restricted bacteria 'Candidatus Liberibacter asiaticus', Candidatus Liberibacter africanus' and 'Candidatus Liberibacter americanus', which are the members of the alpha subdivision of the proteobacteria (Jagoueix et al 1994).…”