Genetic diversity among Jatropha species as revealed by RAPD markers

Ram, S. Ganesh; Parthiban, K. T.; Kumar, Randhir; Venkatesan, T.; Paramathma, M.

doi:10.1007/s10722-007-9285-7

Cited by 122 publications

(51 citation statements)

References 24 publications

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“…In the present study, the highest rate of genetic similarity was found within J. curcas and then between J. curcas and J. integerrima, which is in agreement with Ganesh et al (2008) and Tanya et al (2011b). EST-SSR primers identified in this study had a good cross species/genera amplification efficiency and thus are useful to identify genetic diversity of J. curcas and its close taxa as well as to choose the desired related species for wide crossing to improve jatropha.…”

Section: Discussionsupporting

confidence: 93%

“…Genetic relatedness among the Jatropha species/genera Many studies were carried out to analyze the genetic relatedness among Jatropha species with markers, such as amplified fragment length polymorphism (Pamidimarri et al 2009), ISSR (Tanya et al 2011b), randomly amplified polymorphic DNA (Ganesh et al 2008;Pamidimarri et al 2009) and SSR (Pamidimarri et al 2011;Tanya et al 2011a). In the present study, the highest rate of genetic similarity was found within J. curcas and then between J. curcas and J. integerrima, which is in agreement with Ganesh et al (2008) and Tanya et al (2011b).…”

Section: Discussionmentioning

confidence: 99%

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Development and characterization of EST-SSR markers from Jatropha curcas EST database and their transferability across jatropha-related species/genus

et al. 2012

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Jatropha curcas (jatropha) is a multipurpose plant with potential as a raw material for biofuel. In the present study, a total of 43,349 expressed sequence tags (ESTs) from J. curcas were searched for type and frequency of simple sequence repeat (SSR) markers. Five thousand one hundred and seventy-five sequences were indentified to contain 6,108 SSRs with 90.8% simple and 9.2% compound repeat motifs. One hundred and sixty-three EST-SSRs were developed and used to evaluate the transferability and genetic relatedness among 4 accessions of J. curcas from China, Mexico, Thailand and Vietnam; 5 accessions of congeneric species, viz. J. gossypiifolia, dwarf J. integerrima, normal J. integerrima, J. multifida, J. podagrica; and Ricinus communis. The polymorphic markers showed 75.56-85.19% transferability among four species of Jatropha and 26.67% transferability across genera in Ricinus communis. Investigation of genetic relatedness showed that J. curcas and J. integerrima are closely related. EST-SSRs used in this study demonstrate a high efficiency of cross species/genera amplification and are useful for identifying genetic diversity of jatropha and its close taxa and to choose the desired related species for wide crossing to improve new varieties of jatropha. The markers can also be further exploited for genetic resource management and genetic improvement of related species/genera through marker-assisted breeding programs.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Development and characterization of EST-SSR markers from Jatropha curcas EST database and their transferability across jatropha-related species/genus

et al. 2012

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“…Dendrogram obtained by the UPGMA method (Figure 2) revealed two major groups at Jatropha germplasm: one made up of wild accession and another group with accessions of J. curcas from Brazil, Mexico, China, and the improved Backcross genotypes. Similar results were reported by Ganesh-Ram et al (2008). Our dendrogram revealed that Mexican genotypes were not grouped in a separately and single cluster.…”

Section: Discussionsupporting

confidence: 90%

“…Molecular markers like RAPD (randomly amplified polymorphic DNA), AFLP (amplified fragment length polymorphism), SSR microsatellites (simple sequence repeat), ISSR (inter-simple sequence repeat), among others have been employed to assess genetic diversity in J. curcas germplasm from different parts of the world (Basha and Sujatha, 2007;Ganesh-Ram et al, 2008;Grativol et al, 2011;Tanya et al, 2011;Sudheer et al, 2011;PecinaQuintero et al, 2014). Most of these studies have reported narrow diversity in germplasm (Basha and Sujatha, 2007;Sun et al, 2008;Shen et al, 2010), independent from the marker system used, which indicate the need to broaden the genetic base in these countries.…”

Section: Introductionmentioning

confidence: 99%

High genetic diversity of Jatropha curcas assessed by ISSR

Hernández¹,

Argollo²,

Franco³

et al. 2017

Genet. Mol. Res.

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ABSTRACT. Jatropha curcas L. is a highly promising oilseed for sustainable production of biofuels and bio-kerosene due to its high oil content and excellent quality. However, it is a perennial and incipiently domesticated species with none stable cultivar created until now despite genetic breeding programs in progress in several countries. Knowledge of the genetic structure and diversity of the species is a necessary step for breeding programs. The molecular marker can be used as a tool for speed up the process. This study was carried out to assess genetic diversity of a germplasm bank represented by J. curcas accessions from different provenance beside interspecific hybrid and backcrosses generated by IAC breeding programs using inter-simple sequence repeat markers. The molecular study revealed 271 bands of which 98.9% were polymorphic with an average of 22.7 polymorphic bands per primer. Genetic diversity of the germplasm evaluated was slightly higher than other germplasm around the world and ranged from 0.55 to 0.86 with an average of 0.59 (Jaccard index). Cluster analysis (UPGMA) revealed no clear grouping as to the geographical origin of accessions, consistent with genetic structure analysis using the Structure software. For diversity analysis between groups, accessions were divided into eight groups by origin. Nei's genetic distance between groups was 0.14. The results showed the importance of Mexican accessions, congeneric wild species, and interspecific hybrids for conservation and development of new genotypes in breeding programs.

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“…Highest genetic similarity and minimum polymorphism was found in Jatropha curcas by RAPD analysis. Basha and Sujatha (2007) and Ram et al (2007). In this work, we compared the applicability of ISSRs and RAPDs as genetic markers to characterize the four Jatropha species genotypes.…”

Section: Discussionmentioning

confidence: 99%

Morphological, Phytochemical and Molecular Characterization on Some Jatropha Species Cultivated in Egypt

El-leel¹

2017

IJPSR

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This study was to screen the morphological, phytochemical attributes and describe the similarity and diversity in terms of RAPD and ISSR profiles of four Jatropha species (J. multifida, J. gossypifolia, J. integrrima and J. curcas). As well, investigate genetic diversity among them for using in conserving at Gene-Banks and genetic improvement. It appeared that show the variation in twenty-three quantitative and qualitative attributes among Jatropha species under Egyptian condition. The results of Pearson correlation among twelve quantitative revealed high significant positive correlations. Negative correlation observed between petiole length and other traits except seed breadth, whereas it was -0.97 when be compared with pod length. In PCA studied, it noted that leaf length, leaf width and petiole length were contributing most to the separation among species. The result showed that all secondary metabolites analyzed were present in all leaves of Jatropha species studied but at different concentrations and it obtained confirm the relatedness of these species and spotlight these important phytochemicals in the species. At molecular study, four RAPD-primers displayed a total of 66 amplified fragments, in which 50 (75.76%) were polymorphic fragments. The number of total amplified fragments scored per primer ranged from 10 (primer OP-D09) to 24 (primer OP-Z03). Thirty-six out of 66 RAPD-PCR fragments were found to be useful as cultivar specific markers. In ISSR analysis, 5 of the ISSR primers generated variable banding patterns. A total of 63 out of 90 ISSR fragments were polymorphic. 36 amplified fragments were considered as cultivar-specific markers. Results of the combination of the banding patterns of both techniques, data exhibited that the most two closely related species were multifida and gossypifolia with the highest similarity index (1.00). On the other hand, the two most distantly related species were curcas and integerrima with no similarity index (0.00). The 9 primers of RAPD and ISSR yielded 113 polymorphic markers that unambiguously discriminated 4 genotypes into three clusters. In conclusion, polymorphisms of both could be used as efficient tools for the detection of similarities and phylogenetic relationships of the studied genotypes, which could be useful in the breeding programs.

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Genetic diversity among Jatropha species as revealed by RAPD markers

Cited by 122 publications

References 24 publications

Development and characterization of EST-SSR markers from Jatropha curcas EST database and their transferability across jatropha-related species/genus

Development and characterization of EST-SSR markers from Jatropha curcas EST database and their transferability across jatropha-related species/genus

High genetic diversity of Jatropha curcas assessed by ISSR

Morphological, Phytochemical and Molecular Characterization on Some Jatropha Species Cultivated in Egypt

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