Genetic Diversity Among Saudi Peganum harmala and Rhazya stricta Populations Using Chemical and ISSR Markers

Abstract

Background: The vernacular name 'Harmal' is used for two plant species in Saudi Arabia, i.e. Peganum harmala L. and Rhazya stricta Decne. Both are important medicinal plants which offer interesting pharmacological properties. Objective: This study aimed to evaluate the genetic diversity among different populations of harmal based on chemical variations of alkaloids and molecular polymorphism. Methods: Total alkaloids were extracted from plants of three populations of each species and estimated by using spectrophotometer and the chemical compounds were analyzed by Gas chromatography mass spectrometry (GC-MS). Molecular polymorphism was estimated by using the Inter Simple Sequence Repeat (ISSR) fingerprints. Results: The results showed that the alkaloids content of R. stricta was higher than P. harmala populations. The GC-MS analysis revealed the presence of (65-53) compounds in R. stricta and P. harmala, and the percentage of polymorphism was found to be 93.2%. Sixteen ISSR primers produced 170 scorable bands with an average of 9.6 bands per primer and 75%-100% polymorphism. The cluster analysis using the unweighted pair-group method of the arithmetic average (UPGMA) method based on combined data of GC-MS and ISSR markers divided the six harmal genotypes into two major groups. Conclusion: The existence of variations in chemical and genetic markers is useful for the selection of potential genotypes for medicinal use, and for breeding lines for medicinal substances production to spare wild plants from uncontrolled harvesting for folk medicine.

ISSR and SCoT for evaluation of hereditary differences of 29 wild plants in Al Jubail Saudi Arabian

“…Molecular markers have been utilized within the development of molecular relation maps, hereditary differences investigations, cultivar identification, and molecular marker-assisted breeding ( Kalia et al, 2011 ). Molecular markers, such as inter simple sequence repeat (ISSR) and start codon targeted (SCoT) have been connected for genetic diversity investigations for a few species of Zygophyllaceae ( Morsy and El Sherbeny, 2015 , Moawed and Ibrahim, 2016 , Abd-Elgawad and Alotaibi, 2019 ). These molecular marker strategies are broadly utilized for hereditary differences examination, as they are basic and can deliver a gigantic number of DNA markers in any test.…”

GC-MS Analysis of Bioactive Compounds Extracted from Plant Rhazya stricta Using Various Solvents

“…Most of the compounds extracted with methanol were alkaloids; similarly, previous data showed the existence of alkaloids in R. stricta [ 15 , 30 ]. Additionally, genetic diversity can affect the plant content of alkaloids [ 31 ]. Alkaloids are a rich source of the materials used for drug discovery and formulation; thus, scientists have examined various alkaloids for their anticancer and antiproliferative activities [ 32 , 33 ].…”