Genetic diversity analysis of Mediterranean faba bean (Vicia faba L.) with ISSR markers

Terzopoulos, P.J.; Bebeli, Penelope J.

doi:10.1016/j.fcr.2008.02.015

Cited by 94 publications

(91 citation statements)

References 33 publications

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“…Therefore, these 3 populations could contribute to enrich the genetic foundation for faba bean breeding in Tunisia. Similar results were commented by Terzopoulos and Bebeli (2008) which suggested introducing some Greek faba bean populations as sources of lines appropriate for the development of synthetic varieties. Liu and Hou (2010) suggested that the classification results of germplasm resources were not only related to the types of markers but also were closely linked to classification method or foundation.…”

Section: Discussionsupporting

confidence: 73%

Genetic variability in Tunisian populations of faba bean (Vicia faba L. var. major) assessed by morphological and SSR markers

Rebaa¹,

Abid²,

Aouida³

et al. 2017

Physiol Mol Biol Plants

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The genetic diversity of 21 faba bean populations was examined using morphological and molecular markers. DNA was extracted from 189 individuals and 8 microsatellite markers were genotyped individually in these 21 populations. A total of 53 alleles were obtained in all populations, with an average of 6.62 alleles per locus. The expected and observed heterozygosity was 0.38 and 0.62 respectively. The average polymorphism index content of SSR markers was 0.61, ranging from 0.31 to 0.81. The unweighted pair group method with arithmetic mean dendrogram clustered all the populations into two groups, each for them subdivided into 3 sub-groups according to geographical origin. Morphological variation showed that the populations were not grouped according to their geographical origin. Therefore, patterns of differentiation of morphological traits did not coincide with molecular differentiation, indicating that morphological variation does not reflect genetic subdivision in studied faba bean populations. Analysis of molecular variance revealed high levels of genetic variation (83%) within population and provides a good base for designing genetic improvement programs. The result of Principal Component Analysis (PCA) revealed that three dimensional principal components (PC1, PC2 and PC3) contributed 40.56% of the total variability and accounted with values of 20.64, 11.22 and 8.70%, respectively. Cluster analysis based on PCA indicated three separate groups of populations. The genetic relationships found between the 21 populations samples were the same in both the PCA and STRUCTURE analysis which support the results observed. These data may serve as a foundation for the development of faba bean breeding programs.

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Section: Discussionsupporting

confidence: 73%

Genetic variability in Tunisian populations of faba bean (Vicia faba L. var. major) assessed by morphological and SSR markers

Rebaa¹,

Abid²,

Aouida³

et al. 2017

Physiol Mol Biol Plants

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“…It is worth mentioning that P38 is a population in which land is worked above 2500 m as indicated in Table 1, which suggests that the altitude factor heavily influences on its genetic expression, making it different. These results agree with reports by Terzopoulos and Bebeli (2008) and Wang et al (2012), who comment that genetic diversity in faba bean is closely associated to the habitat in which it grows, geographic origin, and ecologic distribution. Group II comprehended 60% of the accessions of the central part, as well as two accessions from the north and south, P8 and P34, respectively.…”

Section: Genetic Variability Between Populationssupporting

confidence: 81%

“…Such results allow distinguishing that primers 848 and ISSR-2M, amplified 20 and 18 total bands and for both 100% were polymorphic, these are dinucleotides of (CA) anchored to tetra-and penta-nucleotides, these two primers and 866 are nucleotide sequences, still unreported by other researchers to amplify polymorphic bands in faba bean (Terzopoulos et al, 2004;Abdel-Razzak et al, 2012) had already assessed a similar (CA) 8G sequence in P1, P2, P3, P4, P5, P6, P8, P9 P7 P10, P11, P12, P13, P14, P15, P16, P17, P18, P19, P34 P20 P23 P21, P22, P24, P25, Greece and Egypt, respectively. By contrast, Terzopoulos and Bebeli (2008), Alghamdi et al (2011), andWang et al (2012) assessing faba bean accessions from Greece, Saudi Arabia, and China, respectively, found higher polymorphism with primers of dinucleotide replications (AG) and (GA) anchored to mononucleotides and trinucleotides; sequences which were also detected in our materials but with fewer total bands. This suggests gene differences between the gathered material assessed in this study and those reported for faba bean cultivars in other countries; such a genetic difference can be attributed to the broad geographic separation there is between the materials.…”

Section: Polymorphism Of Issr Markersmentioning

confidence: 52%

“…Using this sort of markers, Wang et al (2012) analyzed 802 faba bean accessions from China and other parts of the world, they found well-defined groupings of accessions according to the geographic region these accessions came from; AbdelRazzak agree et al (2012) studied 10 faba bean varieties from Egypt finding differences between cultivars; Alghamdi et al (2011) assessed 34 faba bean populations detecting variability related to the gathering sites; finally, Terzopoulos and Bebeli (2008) analyzed the genetic diversity of 20 local populations from the Mediterranean, finding high variation levels in them and at least two genetically different groups.…”

Section: Introductionmentioning

confidence: 99%

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Genetic variability analysis of faba bean accessions using Inter-simple sequence repeat (ISSR) markers

Salazar-Laureles¹,

Pérez-López²,

González-Huerta³

et al. 2015

Chilean J. Agric. Res.

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Faba bean crop (Vicia faba L.) is important in Mexico, it is a legume rich in protein and its market price is higher than those of maize and bean; however, in recent years there has been a significant decline in its performance. The aim of this study was to determine the genetic variability and analyze the relationship between 39 faba bean accessions using inter simple sequence repeats (ISSR) markers. Accessions of faba bean were collected according to their best phenotypic characteristics, later they were molecularly characterized. With the obtained band registration, a binary data matrix was built to perform the corresponding statistical analysis. The utilized ISSR markers produced 142 fixed and repeatable bands, of which 134 were polymorphic. The values of resolution power (Rp), polymorphic information content (PIC), and marker index (MI), respectively indicated that primer 848 was the most efficient to analyze genetic variability with values of 12.8, 0.40, and 8.06, respectively, followed by primers 857 and ISSR2M. Genetic distances oscillated between 0.38 and 0.83, and verified the groupings observed in the dendrogram, which indicates high variability at the level of DNA among the analyzed genotypes, observing six defined groups according to UPGMA analysis. In the analysis of main components the registered groupings were determined by the origin of the gathering. The use of ISSR markers was efficient to characterize at the level of DNA the assessed bean accessions, indicating the existence of variability, the identified contrasting accessions can be utilized in genetic improvement programs aimed at solving the needs of the producers.

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“…However, this very high number of polymorphic bands was a result of inter-species characterization. Similarly to our study, previous intraspecies studies have shown a high level of polymorphism detected by ISSR markers: In Chinese barley accessions (98.13%) (Hou et al, 2005), in Portuguese bread wheat cultivars (98.5%) (Carvalho et al, 2010) and in Mediterranean faba bean cultivars (98.9%) (Terzopoulos and Bebeli, 2008). Obtaining a high number of polymorphic ISSR primers is greatly useful.…”

Section: Discussionmentioning

confidence: 75%

Genetic Diversity Analysis of Safflower (Carthamus tinctorius) Accessions from Different Geographic Origins using ISSR Markers

Houmanat¹,

Charafi²,

Mazouz³

et al. 2016

IJAB

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Safflower (Carthamus tinctorius) is an oilseed crop with high quality vegetable seed oil. A set of 55 accessions from different geographical origins were introduced to constitute a breeding program germplasm. These materials were characterized by inter-simple sequence repeat (ISSR) as a simple method to reveal polymorphism among them. ISSR analysis was carried out with 13 primers. After agarose gels analysis, ISSR banding patterns were converted into binary data of presence-non presence and matrices were processed. Genetic distances between all pairwise combinations of the accessions were calculated using similarity of simple coefficient matching (SCM). Dendrogram of studied accessions was constructed using the UPGMA (unweighted pair group method arithmetic average) method. A high number of reproducible ISSR bands exhibited a high percentage of polymorphism (69.64%), indicating a high genetic diversity among the material studied. The lowest similarity coefficient was 0.462, observed between accessions 109 and 135, whilst the highest one was 0.962, observed between accessions 68 and 126, with an average of 0.726. Dendrogram similarity relationships revealed some clusters according to different genetic pools. The ISSR markers were very informative and exhibited a high level of polymorphism. The findings of this study would be useful for safflower breeding program in Morocco as well as in other countries of the world.

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Genetic diversity analysis of Mediterranean faba bean (Vicia faba L.) with ISSR markers

Cited by 94 publications

References 33 publications

Genetic variability in Tunisian populations of faba bean (Vicia faba L. var. major) assessed by morphological and SSR markers

Genetic variability in Tunisian populations of faba bean (Vicia faba L. var. major) assessed by morphological and SSR markers

Genetic variability analysis of faba bean accessions using Inter-simple sequence repeat (ISSR) markers

Genetic Diversity Analysis of Safflower (Carthamus tinctorius) Accessions from Different Geographic Origins using ISSR Markers

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