Genetic Diversity and Conservation of Bulgarian Endemic<i>Verbascum tzar-borisii</i>(Scrophulariaceae)

Petrova, Galya; Petrov, Stefan; Delcheva, Malina; Bancheva, Svetlana

doi:10.5735/085.054.0614

Cited by 9 publications

(6 citation statements)

References 46 publications

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“…It also shows that the populations had a simple pedigree and that the genetic exchange between each pair of populations was low. Similar results were also observed in studies with endemic plant species with small and isolated populations [14,52].…”

Section: Discussionsupporting

confidence: 88%

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Assessment of the genetic diversity of a critically endangered species Centaurea amaena (Asteraceae)

Atasagun

2022

ARCH BIOL SCI BELGRA

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Centaurea amaena is an endemic and endangered species listed as CR (critically endangered) in Turkey. ISSR markers were used to detect the level of genetic diversity in two natural populations of C. amaena. A total of 50 ISSR primers were used and 13 primers producing polymorphic and reproducible products were selected. These primers yielded 102 amplified discernible loci, of which 80 (78%) were polymorphic. A high level of genetic diversity was detected both at population and species levels; the effective number of alleles (Ne) was 1.544, the observed number of alleles (Na) was 1.784, the Nei?s genetic diversity (H) was 0.306, and Shannon's information index was 0.447. The established gene flow (Nm) was 2.329, indicating a high migration rate between the populations. A moderate level of genetic differentiation (GST: 0.176) was also observed. Analysis of molecular variance (AMOVA) revealed that 24.89% of the total genetic diversity resided among populations, while 75.10% was within the populations. Cluster analysis showed that samples from the same locality clustered together and there was no cross-clustering between the samples. The patterns of genetic variation indicate that existing C. amaena populations should be conserved.

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Section: Discussionsupporting

confidence: 88%

“…ISSR are usually dominant markers and allow more stringent amplification [12,13]. ISSR markers have effectively been used in the fields of genetic diversity, phylogenetic analysis and evolutionary biology [14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Assessment of the genetic diversity of a critically endangered species Centaurea amaena (Asteraceae)

Atasagun

2022

ARCH BIOL SCI BELGRA

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“…Therefore, widespread distribution of the studied MLACs in Iran may have been led to high genetic diversity. Biological characteristics, the pattern of distribution, mating system, life cycle, and propagation method are usually the main factors impacting genetic diversity [57,58] . Due to the high variation in ploidy levels and hybrids between the species, the mint genus has a high genetic diversity [19] .…”

Section: Resultsmentioning

confidence: 99%

“…Mean values refer to colors from minimum displayed in bright green to maximum represented with red. b) PDA‐HPLC diagram that shows the relative values of 3.4‐dihydroxybenzoic acid, [2] 25‐dihydroxybenzoic acid, [2] chlorogenic acid, [2] vanillic acid, [50] caffeic acid (5), p‐ coumaric acid, [58] ferulic acid, [50] rosmarinic acid, [2] and rutin [2] in the studied accession of Mentha longifolia L. c) Biplot of PCA analysis based on targeted PDA‐HPLC analyses for phenolic compounds.…”

Section: Resultsmentioning

confidence: 99%

Association of Genetic Structure and Diversity in Iranian Wild Germplasms ofMentha longifoliaL. Based on Phenotypical, Biochemical, and Molecular Markers

Araghi

Nemati

Azizi

et al. 2021

Chemistry & Biodiversity

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Mentha longifolia L. is well‐known to be one of the most pervasive wild‐growing species of the Lamiaceae family, which has extensive beneficial properties in the fields of pharmacology and biological products. In the present study, the correlation between Inter‐simple sequence repeat (ISSR) markers and morpho‐chemical parameters of twenty different M. longifolia accessions (MLACs) were assessed. The geographic information system (GIS) has been employed to interpret the original habitat of the accessions in Iran. ISSR analysis indicated a remarkable difference in the studied accessions, segregated them into three main groups, constructed by an unweighted pair‐group method with arithmetic (UPGMA) and principal coordinate analysis (PCoA). A total of 89 bands were generated by 12 ISSR primers, among which 82 (91.97 %) of them were polymorphic. The cluster analysis based on agro‐morphological data scattered MLACs into two main groups. The essential oils (EOs) were analyzed through GC/FID/MS, and four chemotypes were characterized according to the major constituents. Pulegone ranged from 0.17 to 69.50 % was the main oil constituent with the highest content. Also, HPLC‐PDA was employed to identify and to quantify the phenolic compounds in the MeOH extracts of MLACs. Heatmap cluster based on phenolic compounds produced three main categories of accessions. The components identified in the extracts were rosmarinic acid, rutin, vanillic acid, ferulic acid, chlorogenic acid, caffeic acid, 3,4‐dihydroxybenzoic acid, 2,5‐dihydroxybenzoic acid, and p‐coumaric acid, which among them rosmarinic acid (RA) varied from 39.16 to 261.55 mg/100 g (DW) as a predominant constituent. Subsequently, multiple regression analyses between ISSR fragments and morpho‐chemical data illustrated considerable relationships in the plant materials. The high variation and correlation observed in metabolic and phenotypic traits of MLACs establish an adequate source to conduct reserves conservation programs.

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“…Stef.‐Gat. were PLY SP = 96% and PLY POP = 69.4%, respectively (Petrova et al 2017b), while V. anisophyllum Murb. was determined as PLY POP = 58.5% (Petrova et al 2017a) and V. davidoffii Murb.…”

Section: Discussionmentioning

confidence: 99%

The assessment of genetic diversity and population structure of Verbascum gypsicola by ISSR markers for conservation purposes

Keser

Yaprak

2022

Nordic Journal of Botany

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The habitats of many plant species are under severe threat, especially from human activities. Therefore, it was aimed to determine the genetic potential of endemic Verbascum gypsicola in Turkey. Samples were taken from 96 individuals belonging to four populations of V. gypsicola. While the percentage of polymorphic locus at the species level was 99.5%, the average percentage of polymorphic locus at the population level was 61.1%, the Nei gene diversity was H = 0.14; the Shannon information index was determined as I = 0.24. At the same time, the total genetic diversity of the species, genetic diversity within the populations, genetic diversity among populations and gene flow values between the populations were determined as HT = 0.14, HS = 0.13, GST = 0.11 and NM = 3.72, respectively. Within‐population variation was 81%, among‐population variation was 8% and interregional variation was 11% according to AMOVA. Results from STRUCTURE analyses revealed two major groupings of populations and some divergence within one of the major groupings. While the genetic diversity at the species level was high, the genetic diversity at the population level was found to be lower than observed for previously studied Verbascum species. The most important reason for this is thought to reductions in population size, driven by environmental damage.

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Genetic Diversity and Conservation of Bulgarian EndemicVerbascum tzar-borisii(Scrophulariaceae)

Cited by 9 publications

References 46 publications

Assessment of the genetic diversity of a critically endangered species Centaurea amaena (Asteraceae)

Assessment of the genetic diversity of a critically endangered species Centaurea amaena (Asteraceae)

Association of Genetic Structure and Diversity in Iranian Wild Germplasms ofMentha longifoliaL. Based on Phenotypical, Biochemical, and Molecular Markers

The assessment of genetic diversity and population structure of Verbascum gypsicola by ISSR markers for conservation purposes

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