Genetic diversity and peculiarity of annual wild soybean (G. soja Sieb. et Zucc.) from various eco-regions in China

Abstract

Annual wild soybean (Glycine soja Sieb. et Zucc.) is believed to be a potential gene source for future soybean improvement in coping with the world climate change for food security. To evaluate the wild soybean genetic diversity and differentiation, we analyzed allelic profiles at 60 simple-sequence repeat (SSR) loci and variation of eight morph-biological traits of a representative sample with 196 accessions from the natural growing area in China. For comparison, a representative sample with 200 landraces of Chinese cultivated soybean was included in this study. The SSR loci produced 1,067 alleles (17.8 per locus) with a mean gene diversity of 0.857 in the wild sample, which indicated the genetic diversity of G. soja was much higher than that of its cultivated counterpart (total 826 alleles, 13.7 per locus, mean gene diversity 0.727). After domestication, the genetic diversity of the cultigens decreased, with its 65.5% alleles inherited from the wild soybean, while 34.5% alleles newly emerged. AMOVA analysis showed that significant variance did exist among Northeast China, Huang-Huai-Hai Valleys and Southern China subpopulations. UPGMA cluster analysis indicated very significant association between the geographic grouping and genetic clustering, which demonstrated the geographic differentiation of the wild population had its relevant genetic bases. In comparison with the other two subpopulations, the Southern China subpopulation showed the highest allelic richness, diversity index and largest number of specific-present alleles, which suggests Southern China should be the major center of diversity for annual wild soybean.

Genetic diversity of wild soybean populations in Dongying, China, by simple sequence repeat analysis

“…These results suggest that wild soybeans collected from the Dongying region of China contained abundant genetic diversity, both at the species and population levels. However, the expected heterozygosity (H E ) and Shannon's information index (I) in this study were lower than those reported in previous studies (Wen et al, 2009;Xu and Li, 2011). The origin of the samples could partly account for these differences.…”

Genetic Divergence of Bradyrhizobium Strains Nodulating Soybeans as Revealed by Multilocus Sequence Analysis of Genes Inside and Outside the Symbiosis Island

“…Although B. yuanmingense nodulating various legumes has been found in several continents, the biovar nodulating soybeans has so far been found only in Asia (2,3,5,50). The observed high diversity of soybeannodulating Bradyrhizobium in Asia is consistent with the presence of wild soybeans and the long history of soybean cultivation in this region (51,52).…”

Analisis Keragaman Genetik Kedelai Introduksi Menggunakan Marka Mikrosatelit

“…Pola pita hasil elektroforesis sampel kedelai introduksi pada gel poliakrilamid 8% yang diamplifikasi menggunakan marka A) GMES3515 dan B) Satt288 (Keterangan: M: DNA Ladder 100 bp). Laboratorium Biologi Molekuler BB Biogen, Januari-Maret 2016 yang berasal dari berbagai negara dengan agroekosistem yang sangat berbeda, sejalan dengan hasil analisis sebelumnya walaupun kultivar yang digunakan berbeda (Zhou et al, 2002;Kuroda et al, 2009;Wen et al, 2009;Kumawat et al, 2015). Hasil analisis keragaman genetik kultivar kedelai pada peneltian ini dapat menjadi dasar dalam karakterisasi plasma nutfah kedelai yang ada dan memberi informasi tentang pemanfaatan dan seleksi pada program pemuliaan kedelai ke depan.…”