Genetic diversity and phylogenetic relationships among microsporidia infecting the silkworm, Bombyx mori, using random amplification of polymorphic DNA: Morphological and ultrastructural characterization

Rao, Soumya; Nath, B. Surendra; Bhuvaneswari, G.; Urs, S. Raje

doi:10.1016/j.jip.2007.05.001

Cited by 10 publications

(11 citation statements)

References 51 publications

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“…The difference in the time taken to reach LT 50 in different microsporidians which ranged from 5.77 to 12.48 days could be due to the differences in the virulence of different microsporidian isolates. These findings are in conformity with the observations of Fujiwara (1979), Kawarabata (2003) and Rao et al (2004Rao et al ( , 2007 who observed dose-dependent mortality by different species of microsporidians infecting the silkworm, B. mori.…”

Section: Discussionsupporting

confidence: 93%

“…However, the small differences in spore width and length should not be considered alone as a taxonomic character because spore size for a given species may vary with the host size (Brooks and Cranford 1972) and is affected by temperature (Maddox and Sprenkel 1978). Takizawa et al (1975), Sato et al (1982), Rao et al (2007) reported in detail the structure, shape and size of different isolates/species of microsporidians identified from the silkworms.…”

Section: Discussionmentioning

confidence: 99%

“…In addition to high virulent in nature, the NIWB-15mb was found to induce heavy infection in certain tissues like fatbody, gut and silkglands. The mid gut is the primary target tissue for Nosema species and infected insect cells are usually filled with diplokaryotic spores (Henry 1971, Rao et al 2007. Most of the microsporidian species are efficiently transmitted both vertically; the direct transfer of infection from parent to progeny and horizontally; the transmission of the pathogens from one individual to another of the same generation (Becnel and Andreadis 1999).…”

Section: Discussionmentioning

confidence: 99%

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Molecular characterization and phylogenetic relationships among microsporidian isolates infecting silkworm, Bombyx mori using small subunit rRNA (SSU-rRNA) gene sequence analysis

Nath

Gupta

Bajpai

2012

Acta Parasitologica

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The life cycle, spore morphology, pathogenicity, tissue specificity, mode of transmission and small subunit rRNA (SSU-rRNA) gene sequence analysis of the five new microsporidian isolates viz., NIWB-11bp, NIWB-12n, NIWB-13md, NIWB-14b and NIWB-15mb identified from the silkworm, Bombyx mori have been studied along with type species, NIK-1s_mys. The life cycle of the microsporidians identified exhibited the sequential developmental cycles that are similar to the general developmental cycle of the genus, Nosema. The spores showed considerable variations in their shape, length and width. The pathogenicity observed was dose-dependent and differed from each of the microsporidian isolates; the NIWB-15mb was found to be more virulent than other isolates. All of the microsporidians were found to infect most of the tissues examined and showed gonadal infection and transovarial transmission in the infected silkworms. SSU-rRNA sequence based phylogenetic tree placed NIWB14b, NIWB-12n and NIWB-11bp in a separate branch along with other Nosema species and Nosema bombycis; while NIWB15mb and NIWB-13md together formed another cluster along with other Nosema species. NIK-1s_mys revealed a signature sequence similar to standard type species, N. bombycis, indicating that NIK-1s_mys is similar to N. bombycis. Based on phylogenetic relationships, branch length information based on genetic distance and nucleotide differences, we conclude that the microsporidian isolates identified are distinctly different from the other known species and belonging to the genus, Nosema. This SSU-rRNA gene sequence analysis method is found to be more useful approach in detecting different and closely related microsporidians of this economically important domestic insect.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Molecular characterization and phylogenetic relationships among microsporidian isolates infecting silkworm, Bombyx mori using small subunit rRNA (SSU-rRNA) gene sequence analysis

Nath

Gupta

Bajpai

2012

Acta Parasitologica

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“…The genus Nosema is subdivided into two clades, the ' true Nosemas ', including Nosema bombycis, and the Vairimorpha clade (Baker et al 1994). Studies on disease phenotype and the phylogenetic relationships of silkworm isolates reveal that both the virulence and the capacity for vertical transmission may vary and parasites from either of the two Nosema clades can be responsible for disease (Rao et al 2005(Rao et al , 2007. Studies on disease phenotype and the phylogenetic relationships of silkworm isolates reveal that both the virulence and the capacity for vertical transmission may vary and parasites from either of the two Nosema clades can be responsible for disease (Rao et al 2005(Rao et al , 2007.…”

Section: F R O M P a S T E U R T O P O L L I N A T O R S: T R A N S Mmentioning

confidence: 99%

“…There clearly are intrinsic differences between microsporidian isolates as revealed by comparison of disease phenotype in a single host, such as the silkworm (Rao et al 2007) or the Gypsy moth (Solter and Maddox, 1998 ;Goetz and Hoch, 2009). There clearly are intrinsic differences between microsporidian isolates as revealed by comparison of disease phenotype in a single host, such as the silkworm (Rao et al 2007) or the Gypsy moth (Solter and Maddox, 1998 ;Goetz and Hoch, 2009).…”

Section: A D a P T I V E T R A N S M I S S I O N S T R A T E G I E S:mentioning

confidence: 99%

The ecology and evolution of microsporidian parasites

Smith

2009

Parasitology

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The phylum Microspora is ancient and diverse and affects a wide range of hosts. There is unusually high use of vertical transmission and this has significant consequences for transmission and pathogenicity. Vertical transmission is associated with low pathogenesis but nevertheless can have significant impact through associated traits such as sex ratio distortion. The majority of microsporidia have mixed transmission cycles and it is not clear whether they are able to modify their phenotype according to environmental circumstances. There is a great need to understand the mechanisms controlling transmission and one of the first challenges for the genomics era is to find genes associated with life cycle stages. Similarly we cannot currently predict the ease with which these parasites might switch between host groups. Phylogenetic analysis suggests that there are strong relationships between Microsporidia and their hosts. However closer typing of parasite isolates, in relation to host range and disease phenotype, is required to assess future environmental risk from these pathogens.

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Ultrastructure and molecular phylogenetics of a new isolate of Pleistophora pagri sp. nov. (Microsporidia, Pleistophoridae) from Pagrus pagrus in Egypt

et al. 2012

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Genetic diversity and phylogenetic relationships among microsporidia infecting the silkworm, Bombyx mori, using random amplification of polymorphic DNA: Morphological and ultrastructural characterization

Cited by 10 publications

References 51 publications

Molecular characterization and phylogenetic relationships among microsporidian isolates infecting silkworm, Bombyx mori using small subunit rRNA (SSU-rRNA) gene sequence analysis

Molecular characterization and phylogenetic relationships among microsporidian isolates infecting silkworm, Bombyx mori using small subunit rRNA (SSU-rRNA) gene sequence analysis

The ecology and evolution of microsporidian parasites

Ultrastructure and molecular phylogenetics of a new isolate of Pleistophora pagri sp. nov. (Microsporidia, Pleistophoridae) from Pagrus pagrus in Egypt

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