Genetic Diversity and Relationships Among Siberian and Far Eastern Larches Inferred from RAPD Analysis

Козыренко, М. М.; Artyukova, E. V.; Реунова, Г. Д.; Levina, Evgeniya A.; Zhuravlev, N.

doi:10.1023/b:ruge.0000024978.25458.f7

Cited by 15 publications

(14 citation statements)

References 14 publications

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“…Levina et al (2008) obtained a total of 36 bands/primer using 12 random primers in different larch populations. On the other hand, the number of RAPD loci obtained is satisfactory (401), compared with Kozyrenko et al (2004), who obtained 457 RAPD loci using 17 random primers in six larch populations.…”

Section: Discussionmentioning

confidence: 96%

Genetic variation and potential genetic resources of several Romanian larch populations

Vilcan¹,

Mihalte²,

Sestraș³

et al. 2017

Turk J Agric For

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decidua (European larch) has a scattered natural area and is present in four geographic zones (Alps, Sudetan Mountains, Carpathians, and Central Poland), where local European larch have sometimes the status of subspecies or varieties (Biswas and Mohri, 1997). In Romania, the larch has a natural range, being gathered into 5 genetic centers: Ceahlau Mountains (1400-1450 m altitude), Ciucas (1000-1450 m), Bucegi (1200-1500 m), Lotru Mountains (670-1900 m with a maximum range in this area), and Apuseni Mountains (600-1200 m), and in many other plantations more than 200,000 cultivated ha (Mihai and Teodosiu, 2009). The origin of seed is mostly unknown, but probably in the old plantation the seed has Austrian origin.Larch breeding objectives include growth, stem form (crookedness), branching, and resistance to larch canker. There is ongoing research for traits such as heartwood formation and quality (Plomion et al., 2001). Larch heartwood is appreciated for its good mechanical properties, its color, and its texture, and it is often used outdoors for its natural durability (Gierlinger et al., 2004).

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Section: Discussionmentioning

confidence: 96%

Genetic variation and potential genetic resources of several Romanian larch populations

Vilcan¹,

Mihalte²,

Sestraș³

et al. 2017

Turk J Agric For

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“…Most of previous studies on DNA variation of Eurasian Larix species focused on non-coding and anonymous regions of nuclear genome and included only a single or very few species Kozyrenko et al, 2004). The objectives of this study are to clarify population differentiation of Eurasian Larix species and to verify their classification.…”

Section: Introductionmentioning

confidence: 99%

Phylogeography of Eurasian Larix species inferred from nucleotide variation in two nuclear genes

et al. 2008

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Larch (Larix Mill.) is one of the most widely distributed tree genera in Eurasia. To determine population structure and to verify classification of five species and three varieties of the Eurasian Larix species, we investigated levels and patterns of nucleotide variation of two nuclear gene regions: the 4-coumarate coenzyme A ligase (4CL) and the coumarate 3-hydroxylase (C3H). In the 4CL region nucleotide diversity at silent sites (π sil ) varied between 0.0020 in L. gmelinii to 0.0116 in L. gmelinii var. japonica and in the C3H region between 0.0019 in L. kaempferi to 0.0066 in L. gmelinii var. japonica. In both gene regions statistically significant population differentiation (F ST ) was detected among adjacent refugial populations of some species suggesting limited gene flow and/or long time isolation of some refugial populations. On the other hand, populations of L. sukaczewii from northwestern Russia, which was glaciated 20,000 years ago showed no differentiation. This result is consistent with recent postglacial origin of these populations. Haplotype composition of some of the investigated Eurasian Larix species suggested that they are considerably diverged. Some haplotypes were unique to individual species. Our results indicate that more intensive sampling especially from known refugial regions is necessary for inferring correct classification of Eurasian Larix species and inferring their postglacial migration.

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“…Only few other similar results of moderate to high levels of population differentiation in Larix species have been reported. In randomly amplified polymorphic DNA, analyses of Larix species (Kozyrenko et al 2004) found an overall G ST = 0.1864. In a study of L. sukaczewii using allozymes, one highly differentiated population from southern Urals (near the location of population 6) was reported, in spite of a low overall population differentiation (F ST =0.028 ;Timerjanov 1997).…”

Section: Population Differentiationmentioning

confidence: 97%

Phylogeography of Larix sukaczewii Dyl. and Larix sibirica L. inferred from nucleotide variation of nuclear genes

Araki

Khatab

Hemamali

et al. 2008

Tree Genetics & Genomes

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We investigated phylogeography of Larix sukaczewii and Larix sibirica using nucleotide variation at three following nuclear gene regions: 5.8 S rDNA including two internal transcribed spacers (ITS), cinnamyl alcohol dehydrogenase (CAD), and phytochrome-O (PHYO). We also included sequences of the 4-coumarate: coenzyme A ligase (4CL) gene region obtained in our recent study. CAD and PHYO showed very low nucleotide variation, but ITS and 4CL had levels of variation similar to those reported for other conifers. Pleistocene refugia have been hypothesized to exist in the Southern Urals and South Central Siberia, where four out of nine of the investigated populations occur. We found moderate to high levels of population differentiation (F ST =0.115-0.531) in some pairwise comparisons suggesting limited gene flow and independent evolution of some refugial populations. In L. sukaczewii, low levels of differentiation were found among populations from areas glaciated during the Pleistocene, indicating their recent origin. Our results also suggest these populations were created by migrants from multiple, genetically distinct refugia. Furthermore, some haplotypes observed in populations from previously glaciated areas were not found in putative refugial populations, suggesting these populations might have contributed little to the extant populations created after the Last Glacial Maximum. Some authors regard L. sukaczewii and L. sibirica as a single species, while others consider them as separate species. The observed conspicuous differences in haplotype composition and distribution between L. sukaczewii and L. sibirica, together with high values of F ST between populations of the two species, appear to support the latter classification.

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Genetic Diversity and Relationships Among Siberian and Far Eastern Larches Inferred from RAPD Analysis

Cited by 15 publications

References 14 publications

Genetic variation and potential genetic resources of several Romanian larch populations

Genetic variation and potential genetic resources of several Romanian larch populations

Phylogeography of Eurasian Larix species inferred from nucleotide variation in two nuclear genes

Phylogeography of Larix sukaczewii Dyl. and Larix sibirica L. inferred from nucleotide variation of nuclear genes

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