Genetic diversity and structure in apple-infesting pests of Carposina sasakii, Grapholita dimorpha and Grapholita molesta in Korea

Sun

et al. 2020

Insects

Numerous gut microbes are associated with insects, but their composition remains largely unknown for many insect groups, along with factors influencing their composition. Here, we compared gut bacterial microbiota of two co-occurring agricultural pests, the peach fruit moth (PFM), Carposina sasakii, and the oriental fruit moth (OFM), Grapholita molesta, collected from different orchards and host plant species. Gut microbiota of both species was mainly composed of bacteria from Proteobacteria, followed by Firmicutes. The two species shared bacteria from the genera Pseudomonas, Gluconobacter, Acetobacter, and Pantoea. When we compared two pairs of PFM and OFM populations collected from the same host species and the same orchard, there is no difference in alpha and beta diversity in gut microbiota. When we compared gut microbiota of the same species and host plant from different orchards, alpha and beta diversity was different in populations of PFM collected from two pear orchards but not in other comparisons. Our study suggests that the two pests share many features of gut microbiota and environment in orchards is a main factor influencing their gut microbiota.

Section: Introductionmentioning

confidence: 99%

Similar Gut Bacterial Microbiota in Two Fruit-Feeding Moth Pests Collected from Different Host Species and Locations

Gong

Sun

et al. 2020

Insects

“…Wang et al, 2017). The highly variable life history of PFM on different host plants may reflect different host-associated biotypes as supported by an analysis of esterase isozyme patterns (L. Hua & Hua, 1995) and random amplified polymorphic DNA (RAPD) (Xu & Hua, 2004), although this is not yet been confirmed by direct studies on population differentiation in PFM (D. H. Kwon, Kim, Kim, Lee, & Yang, 2017;J. Wang et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Chromosome-level genome of the peach fruit moth Carposina sasakii (Lepidoptera: Carposinidae) provides a resource for evolutionary studies on moths

Song

Yue

et al. 2020

Preprint

The peach fruit moth (PFM), Carposina sasakii Matsumura, is a major phytophagous orchard pest widely distributed across Northeast Asia. Here, we report the chromosome-level genome for the PFM, representing the first genome for the family Carposinidae, from the lepidopteran superfamily Copromorphoidea. The genome was assembled into 404.83 Mb sequences using PacBio long-read and Illumina short-read sequences, including 275 contigs, with a contig N50 length of 2.62 Mb. All contigs were assembled into 32 linkage groups assisted by the Hi-C technique, including 30 autosomes, a female specific W chromosome and a Z chromosome. BUSCO analysis showed that 98.2% genes were complete and 0.4% of genes were fragmented, while 1.4% of genes were missing in the assembled genome. In total, 23,218 protein-coding genes were predicted, of which 82.72% were functionally annotated. Because of the importance of diapause triggered by photoperiod in PFM, five circadian genes in the PFM as well as in the other related species were annotated, and potential genes related to diapause and photoperiodic reaction were also identified from transcriptome sequencing. In addition, manual annotation of detoxification gene families was undertaken and showed a higher number of ABC and GST genes in PFM than in most other lepidopterans, in contrast to a lower number of UGT genes, suggesting different detoxication pathways in this moth. The high-quality genome provides a resource for comparative evolutionary studies of this moth and its relatives within the context of radiations across Lepidoptera.

“…The highly variable life history of PFM on different host plants may reflect different host‐associated biotypes as supported by an analysis of esterase isozyme patterns (Hua & Hua, 1995) and random amplified polymorphic DNA (RAPD; Xu & Hua, 2004), although this is not yet been confirmed by direct studies on population differentiation in PFM (Kwon et al., 2017; Wang et al., 2015). Previous studies have shown that detoxification and chemosensory genes are associated with host adaptation of insects (Heckel, 2018; Rane et al., 2019; Wan et al., 2019).…”

Section: Introductionmentioning

confidence: 99%

Chromosome‐level genome of the peach fruit moth Carposina sasakii (Lepidoptera: Carposinidae) provides a resource for evolutionary studies on moths

Molecular Ecology Resources

Song

Yue

et al. 2020

The peach fruit moth (PFM), Carposina sasakii Matsumura, is a major phytophagous orchard pest widely distributed across Northeast Asia. Here, we report the chromosome‐level genome for the PFM, representing the first genome for the family Carposinidae, from the lepidopteran superfamily Copromorphoidea. The genome was assembled into 404.83 Mb sequences using PacBio long‐read and Illumina short‐read sequences, including 275 contigs, with a contig N50 length of 2.62 Mb. All contigs were assembled into 31 linkage groups assisted by the Hi‐C technique, including 30 autosomes and a Z chromosome. BUSCO analysis showed that 98.3% of genes were complete and 0.4% of genes were fragmented, while 1.3% of genes were missing in the assembled genome. In total, 21,697 protein‐coding genes were predicted, of which 84.80% were functionally annotated. Because of the importance of diapause triggered by photoperiod in PFM, five circadian genes in the PFM as well as in the other related species were annotated, and potential genes related to diapause and photoperiodic reaction were also identified from transcriptome sequencing. In addition, manual annotation of detoxification gene families was undertaken and showed a higher number of glutathione S‐transferase (GST) gene in PFM than in most other lepidopterans, in contrast to a lower number of uridine diphosphate (UDP)‐glycosyltransferase (UGT) gene, carboxyl/cholinesterases (CCE) gene and cytochrome P450 monooxygenase (P450) gene, suggesting different detoxication pathways in this moth. The high‐quality genome provides a resource for comparative evolutionary studies of this moth and its relatives within the context of radiations across Lepidoptera.