Genetic diversity and substructuring of the Hungarian merino sheep breed using microsatellite markers

Loukovitis, Dimitrios; Szabó, Mária; Chatziplis, Dimitrios; Monori, István; Kusza, Szilvia

doi:10.1080/10495398.2022.2042307

Cited by 7 publications

(2 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Meanwhile, Rashamol et al (2019) showed no significantly different expression of IL10 in heat stress and non-heat stress Malabari goats, demonstrating strong resilience to heat stress, notably in the ability to maintain the innate immune response, which is consistent with the expression of IL10 in Hungarian Merino in this study. Further explained by Loukovitis et al (2022) that Merino was initially brought to Hungary in the 17th century, giving them plenty of time to acclimatize to the country's semi-arid environment and pastures, although as a imported multipurpose breed, Hungarian Merino has undergone numerous improvements programs since then by crossing them with various Merino and Merino-derived breeds. In contrast, because of its historical value, the Hungarian indigenous Tsigai has been included in the national gene conservation program in order to preserve its original qualities, particularly the traits that allowed them to thrive in a specific Hungarian environment.…”

Section: Discussionmentioning

confidence: 99%

Hungarian indigenous Tsigai, a promising breed for excellent heat tolerance and immunity

Astuti

Bagi

Bodrogi

et al. 2023

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Hungarian indigenous Tsigai, a promising breed for excellent heat tolerance and immunity

Astuti

Bagi

Bodrogi

et al. 2023

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

“…During the primer design process, factors such as amplicon size, GC content, absence of self-complementarity (hairpins), and absence of complementarity to the other primer pair (more than four base pairs at the 3′ end) were taken into consideration. All 99 forward primers (75 original and 21 designed) used for testing were synthesized and ordered with one of the four universal adapters [ 40 ]. These adapters allowed for the use of fluorescent end-labeling in subsequent analyses ( Table S4 ).…”

Section: Methodsmentioning

confidence: 99%

A Selection of 14 Tetrameric Microsatellite Markers for Genetic Investigations in Fallow Deer (Dama dama)

Zorkóczy

Turi

Wagenhoffer

et al. 2023

Animals

View full text Add to dashboard Cite

The fallow deer (Dama dama) represents significant game management value globally, and human activities are significantly impacting the species. Besides the positive effects, these activities can threaten its existence, health, and value. The aim of the authors was to develop a tetranucleotide microsatellite panel that could be clearly interpreted and used for genetic testing of fallow deer. Such a panel did not exist until now and could be particularly useful in the field of conservation genetics and forensics. A total of 99 tetrameric microsatellites, originally designed for related deer species, were tested on 20 fallow deer individuals from five Hungarian sampling areas. Original and newly designed primers were used to amplify the microsatellite regions using previously published or optimized PCR protocols. The lengths and sequences of specific amplicons were detected using capillary electrophoresis, and the rate of polymorphism was determined. Altogether, 80 markers provided PCR products of adequate quality and quantity. Among them, 15 markers proved to be polymorphic (2–5 alleles/locus), and 14 tetrameric markers were selected for further analysis. Statistical calculations showed that the selected polymorphic microsatellites can potentially enable key individualization in many areas of wildlife and population genetics, thus protecting the species.

show abstract

Genetic polymorphism in European and African sheep breeds reared in Hungary based on 48 SNPs associated with resistance to gastrointestinal parasite infection using KASP-PCR technique

Astuti

Gavojdian

Ilie

et al. 2023

Trop Anim Health Prod

Self Cite

View full text Add to dashboard Cite

This pilot study used an alternative and economically efficient technique, the Kompetitive Allele-Specific Polymerase Chain Reaction (KASP-PCR) to examine 48 SNPs from 11 parasite-resistance genes found on 8 chromosomes in 110 animals from five sheep breeds reared in Hungary; Hungarian Tsigai, White Dorper, Dorper, Ile de France, and Hungarian Merino. Allele and genotype frequencies, fixation index, observed heterozygosity, expected heterozygosity, F statistic, and their relationship with the Hardy–Weinberg equilibrium (WHE) and the polymorphic information content (PIC) were determined, followed by principal component analysis (PCA). As much as 32 SNPs out of the 48 initially studied were successfully genotyped. A total of 9 SNPs, 4 SNPs in TLR5, 1 SNP in TLR8, and 4 SNPs in TLR2 genes, were polymorphic. The variable genotype and allele frequency of the TLRs gene indicated genetic variability among the studied sheep breeds, with the Hungarian Merino exhibiting the most polymorphisms, while Dorper was the population with the most SNPs departing from the HWE. According to the PIC value, the rs430457884-TLR2, rs55631273-TLR2, and rs416833129-TLR5 were found to be informative in detecting polymorphisms among individuals within the populations, whereas the rs429546187-TLR5 and rs424975389-TLR5 were found to have a significant influence in clustering the population studied. This study reported a moderate level of genetic variability and that a low to moderate within-breed diversity was maintained in the studied populations.

show abstract

Genetic diversity and substructuring of the Hungarian merino sheep breed using microsatellite markers

Cited by 7 publications

References 26 publications

Hungarian indigenous Tsigai, a promising breed for excellent heat tolerance and immunity

Hungarian indigenous Tsigai, a promising breed for excellent heat tolerance and immunity

A Selection of 14 Tetrameric Microsatellite Markers for Genetic Investigations in Fallow Deer (Dama dama)

Genetic polymorphism in European and African sheep breeds reared in Hungary based on 48 SNPs associated with resistance to gastrointestinal parasite infection using KASP-PCR technique

Contact Info

Product

Resources

About