Genetic Diversity Assessment of Trollius Accessions in China by RAPD Markers

Li, Yong; Ding, Wan Long

doi:10.1007/s10528-009-9290-y

Cited by 13 publications

(9 citation statements)

References 14 publications

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“…Low expense, efficiency in developing large number of DNA markers in a short time, and requirement of basic molecular equipment has made the RAPD technique valuable although band reproducibility, problems of comigration, and scoring errors are a major problem. The technique has been applied in many plant groups such as Glycyrrhiza [42], Atractylodes [43,44], Astragalus [45], Amomun [46], Scutellaria [47], Panax [48][49][50][51], Aconitum [52], Ginkgo [53], Anectochilus [54], Lycium [55], Angelica [56], Bupleurum [57], Dendrobium [58], Magnolia [59], Asarum [60], Apocynum [61], Trollius [62], Phyllanthus [63], Indigofera [64], Coptis [65], Codonopsis [66], Taraxacum [67], Elephantopus [68], and Rehmannia [69]. AP-PCR (arbitrary polymerase chain reaction): AP-PCR (or arbitrarily chosen primers ACP-PCR) is a special case of RAPD using single primers approximately 10-50 bp in length [39].…”

Section: Pcr-based Methodsmentioning

confidence: 99%

New Aspects of DNA-based Authentication of Chinese Medicinal Plants by Molecular Biological Techniques

Heubl¹

2010

Planta Med

136

104

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Section: Pcr-based Methodsmentioning

confidence: 99%

New Aspects of DNA-based Authentication of Chinese Medicinal Plants by Molecular Biological Techniques

Heubl¹

2010

Planta Med

136

104

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“…Several procedures have been developed to counteract the problems derived from the dominance inheritance pattern of some molecular markers including RAPD (i.e., for each biallelic locus the occurrence of a band can be related with two different genotypes) making dominant markers a reliable procedure to infer the genetic structure (Isabel et al 1995;Aagaard et al 1998;Ferreyra et al 2007;Boulila et al 2010;Li and Ding 2010;Muneer et al 2011).…”

Section: Genetic Analysismentioning

confidence: 99%

Genetic Structure of the Carnivorous Plant Pinguicula moranensis (Lentibulariaceae) on the Transvolcanic Mexican Belt

Alcalá

Domínguez

2011

Biochem Genet

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Most species of Pinguicula present a montane distribution with populations located at high altitudes. In this context, we proposed that populations of Pinguicula species could be genetically differentiated even at a local scale. This study supported that prediction, as a RAPD-based analysis of molecular variance revealed a high degree of genetic structure (Φ (st) = 0.157, P = 0.001) and low gene flow (Nm = 1.0) among four central populations of Pinguicula moranensis in Mexico, with a maximum geographic separation of about 140 km. The four populations also exhibited high levels of genetic diversity (mean Nei's genetic diversity = 0.3716; % polymorphism = 95.45%). The evolutionary implications of the genetic structure found in P. moranensis for other species in the genus are discussed in the context of the naturally fragmented distribution and a set of life history traits shared by most Pinguicula species that could promote geographic isolation and limited gene flow.

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“…This is simple, relatively inexpensive, and has been frequently employed to analyze the intra-and inter-specific genetic variation in plants and animals (Yi et al, 2009;Li and Ding, 2010). To overcome the reproducibility problem associated with the conventional RAPD marker, it has been converted into SCAR (Paran and Michelmore, 1993).…”

Section: Discussionmentioning

confidence: 99%

Efficacy of random primer-pair arrays in plant genome analysis: a case study of Cucumis (Cucurbitaceae) for identification of wild and cultivated species

Sk²,

Rajkumari³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. The efficacy of random primer-pair arrays compared to conventional RAPD method with a single decamer primer was evaluated using DNA from two species of Cucumis. The banding patterns of amplicons revealed enhanced utility of primer-pair arrays over conventional RAPDs, producing more bands and a higher degree of polymorphism, both at intraand inter-specific levels. Amplification produced by both methods clearly distinguished a wild from a cultivated species of the genus Cucumis. The main advantage of the primer-pair RAPD over single-primer-based RAPD is the increase in the number of reactions and amplification products in the form of novel/unique bands with a limited number of primers. It also enables the generation of reliable amplicons with a large number of polymorphic bands, which can be linked to gene-governing traits, allowing sequence-characterized partial genome analysis.

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Genetic Diversity Assessment of Trollius Accessions in China by RAPD Markers

Cited by 13 publications

References 14 publications

New Aspects of DNA-based Authentication of Chinese Medicinal Plants by Molecular Biological Techniques

New Aspects of DNA-based Authentication of Chinese Medicinal Plants by Molecular Biological Techniques

Genetic Structure of the Carnivorous Plant Pinguicula moranensis (Lentibulariaceae) on the Transvolcanic Mexican Belt

Efficacy of random primer-pair arrays in plant genome analysis: a case study of Cucumis (Cucurbitaceae) for identification of wild and cultivated species

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