Genetic Diversity Characterization of Pleurotus strains by Random Amplified Polymorphic DNA Fingerprinting

Yadav, Mayank; Chandra, Ram; Singh, Hemant; Yadav, SK; Naik, Sushreeta; Dhakad, P. K.

doi:10.20546/ijcmas.2017.605.136

Cited by 4 publications

(2 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RAPD have been used to examine material from the genera Agaricus , Coprinus and Lentinula [14]. Genetic Diversity Characterization of Pleurotus strains by Random Amplified Polymorphic DNA Fingerprinting has been performed by various workers [15], [16]. Fruiting body observations provide information about the fungi on the surface.…”

Section: Introductionmentioning

confidence: 99%

Inter and intraspecific genetic diversity (RAPD) among three most frequent species of macrofungi ( Ganoderma lucidum , Leucoagricus sp. and Lentinus sp.) of Tropical forest of Central India

Dwivedi

Singh

Chauhan

et al. 2018

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

In present study seven RAPD primers were used to access the diversity within and among twelve populations of three mushroom species Ganoderma lucidum, leucoagaricus sp. and Lentinus sp. Total of 111 bands were scored by 7 RAPD primers in 30 accessions of three mushroom species collected from different sampling sites of central India. Total 111 bands were generated using seven primers which were F-1, OPG-06, OPC-07, OPD-08, OPA-02, OPD-02, OPB-10. All 111 bands were polymorphic in nature (100%). Therefore, it revealed that the used primers had sufficient potency for population studies and 30 accessions had higher genetic differences among each other. In best of the knowledge, this is the first report, which accesses the genetic diversity between three mushroom species (Gd Ganoderma lucidum, Lg Leucoagaricus sp., Ls Lentinus). The polymorphic percentage ranged from 3.60 to 23% within twelve populations, while polymorphic percentage among group was 40.56, among population within groups was 41.12 and within population was 18.32. This indicated that the genetic diversity within the population was very low, but slightly higher in the populations of three species. Among three groups representing Gd., Lg and Ls, Among populations within groups shown highest percentage of variation (Pv = 41.12) while within populations, the lowest percentage of variation (18.32) was observed. This result also support that the highest genetic variation was present among groups in comparison to among the population within a species and lowest genetic variation was observed within the population.

show abstract

Section: Introductionmentioning

confidence: 99%

Inter and intraspecific genetic diversity (RAPD) among three most frequent species of macrofungi ( Ganoderma lucidum , Leucoagricus sp. and Lentinus sp.) of Tropical forest of Central India

Dwivedi

Singh

Chauhan

et al. 2018

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

show abstract

“…For each primer, the average yield was 16.83 DNA bands per primer (Table 1). Yadav et al (2017) reported that 79.5% of RAPD bands of Pleurotus strains were polymorphic, and Pawlik et al (2012) reported that 21 strains of Pleurotus obtained 90.4% polymorphism. The polymorphic band obtained from this current study is higher than those two previous studies which can be caused by the variety of primers used and the higher genetic variation of the strains used in this study.…”

Section: Rapd Profilesmentioning

confidence: 99%

Genetic diversity of Pleurotus ostreatus (Jacq.) P. Kumm. strains in Java based on random amplified polymorphic DNA markers

et al. 2021

View full text Add to dashboard Cite

Abstract. Ekowati N, Mumpuni A, Muljowati JS, Ratnaningtyas NI, Maharning AR. 2021. Genetic diversity of Pleurotus ostreatus (Jacq.) P. Kumm. strains in Java based on Random Amplified Polymorphic DNA markers. Biodiversitas 22: 3488-3493. Genetic variation in a fungal population can occur due to mutation and recombination, resulting in changes in the nucleotides that encode specific DNA sequences. Strains with a high genetic distance and good production capabilities can be used to develop genetic breeding. This study aimed to investigate genetic relationship among Pleurotus ostreatus strains cultivated in Java (Bogor, Cianjur, Tasikmalaya, Purwokerto, Yogyakarta, Tawangmangu, Malang, and Madiun) based on random amplified polymorphic DNA (RAPD) markers. The research method consisted of DNA isolation and DNA amplification using six primers, i.e. OPA2, OPA3, OPA4, OPA7, OPA9, and OPA10. DNA band data were analyzed using NTSYSpc21 software to determine the level of genetic similarity, based on the Unweighted Pair Group Method with Arithmetic Average Algorithm (UPGMA). In all, 101 amplified DNA bands were obtained, with sizes ranging from 136 to 2320 bp and 96.0% of the bands were polymorphic. Based on cluster analysis, it shows that three clusters were formed. There were genetic variations and relationships among eight P. ostreatus strains in Java with a genetic similarity varying from 37-98%.

show abstract

Development of improved strain in species of Pleurotus by gamma irradiation

Jyothi¹,

Thara

2021

J Food Sci Technol

View full text Add to dashboard Cite

Genetic Diversity Characterization of Pleurotus strains by Random Amplified Polymorphic DNA Fingerprinting

Cited by 4 publications

References 3 publications

Inter and intraspecific genetic diversity (RAPD) among three most frequent species of macrofungi ( Ganoderma lucidum , Leucoagricus sp. and Lentinus sp.) of Tropical forest of Central India

Inter and intraspecific genetic diversity (RAPD) among three most frequent species of macrofungi ( Ganoderma lucidum , Leucoagricus sp. and Lentinus sp.) of Tropical forest of Central India

Genetic diversity of Pleurotus ostreatus (Jacq.) P. Kumm. strains in Java based on random amplified polymorphic DNA markers

Development of improved strain in species of Pleurotus by gamma irradiation

Contact Info

Product

Resources

About