Genetic diversity in the Indian population of Penaeus monodon (Fabricius, 1798) as revealed by mtDNA sequence analysis

Abstract

Genetic diversity in the Indian population of the tiger shrimp Penaeus monodon was determined by using partial sequence data of the mitochondrial DNA (mtDNA) control region (D‐loop) and the 16S rRNA gene. Eight populations from different geographical locations (Mumbai, Kochi, Mangalore, Kakinada, Gopalpur, Chilika, Paradeep and Andaman) were collected and analysed. The amplified polymerase chain reaction products of size 577 bp for the control region and 472 bp for 16S rRNA were sequenced in both directions and data were analysed through clustal, arlequin, mega and phylip. A significant genetic structure was found among the Indian populations. The mtDNA control region proved to be a powerful marker in comparison with 16S rRNA for population studies of this species. The east coast population was more genetically diverse than the west coast. The Andaman population was found to be the most diverse among all the populations. The populations on the west coast were found to be genetically more structured and differentiated than the populations on the east coast. The results revealed a high level of genetic diversity and also distinct population structuring of P. monodon, suggesting great possibilities of genetic improvement for growth and other economic traits.

GENETIC STATUS, SOURCE AND ESTABLISHMENT RISK OF THE GIANT TIGER SHRIMP (PENAEIDAE: Penaeus monodon), AN INVASIVE SPECIES IN COLOMBIAN CARIBBEAN WATERS

“…Even when we compare the data from the invasive population with other studies of native populations (e.g. Kumar et al, 2007;You et al, 2008;Zhou et al, 2009;Walther et al, 2011;Waqairatu et al, 2012), their genetic diversity remains low (Table 3). Genetic diversity loss associated with a founder effect has been observed in other invading organisms.…”

“…Furthermore, there is evidence that Colombia imported a stock of P. monodon for aquaculture from Brazil in 1987 (Álvarez-León andGutiérrez-Bonilla, 2007) and the latter made two introductions of this species: the first, from Philippines in the 70's by Empresa de Pesquisa Agropecuária do Estado do Rio Grande do Norte (EMPARN: a State research division dedicated to farming, cattle raising and aquaculture studies) (Madeiros et al, 2006;Leão et al, 2011) and the second, from Taiwan in 1984 by a cultivation enterprise in Valença (Bahia STATE) (Madeiros et al, 2006).…”

Molecular Characterization of Economically Important Penaeid Population in South East Coast of India

“…This could be due to a large fluctuation in the physic-chemical parameters in the east coast because of several river drainage in the east coast (Kumar et al, 2007). Metapenaeus dobsoni (Miers, 1878) populations from the west coast of India state Kerala and Maharashtra clustered together, but Tamil Nadu formed a separate group as east coast of India (Lakra et al, 2010).…”

“…Genetic diversity and geographic differentiation of the P. monodon mitochondrial COI sequences (Kumar et al, 2007;Khamnamtong et al, 2009). Randomly amplified polymorphic DNAs (Aubert and Lithtner, 2000;Mishra et al, 2009;Lakra, 2010;Rezvani et al, 2011) and microsatellite (Wolfus et al, 1997;Brooker et al, 2000;Supungal et al, 2000;Maggioni et al, 2003;Robainas-Barcia and García-Machado, 2012;Mandal et al, 2012).…”

Assessment of genetic variation in outbreed and inbreed giant tiger shrimp (Penaeus monodon Fabricius, 1798) using ISSR marker

“…Population genetic data of Penaeus monodon based on several markers have been conducted, such as allozymes [4], mitochondrial DNA (mtDNA) fragment length polymorphism [5], randomly amplified polymorphic DNA (RAPD) [5,6], microsatellites [7,8,9], and sequence data [9,10,11]. However, it is essential to conduct wider genetic variation assessment using another molecular marker to provide holistic polymorphism database.…”