Genetic diversity of Amorphophallus titanum in Bengkulu, Indonesia based on RAPD markers

Abstract

Hidayati NR, Suranto, Sajidan. 2018. Morphological characteristics and isozyme banding patterns of Cucurbita moschata at different altitudes. Biodiversitas 19: 1683-1689. Aims of this research were to investigate the morphological character and isozyme banding patterns of Cucurbita moschata plants grown at three different altitudes. Samples in this study consisted of leaf, stem, and flowers. The morphological characters were conducted by direct observation in the field and analyzed descriptively as well as statically by one way ANOVA. The isozyme bands appearance of esterase and peroxidase of leaf samples were conducted using polyacrylamide gel electrophoresis (PAGE). Qualitative approach was used to analyze the presence and the absence of isozyme bands, while Retardation factor (Rf) was used to analyze quantitatively. The results showed that most plants grown at middle altitude (351-750 m asl.) were well-developed in terms of length of leaves, stems and flowers. Accordingly, the isozyme banding pattern of peroxidase was also found varied in plants grown at middle altitudes from which the presence of very unique bands was detected. Conversely, the band detected in plants grown at the lower and the highest altitudes was similar in term of band's number but it was different in the quality of the bands. Meanwhile, esterase isozyme banding pattern of plants grown at the lower and higher altitude had more bands than the middle altitude. Based on this result it is obvious that the isozyme data could be used to support in understanding the diversity morphological characters of plants grown in three different altitudes. This early result suggests that altitudes as a crucial factor in contributing the expression of isozyme appearance, which is useful for further pumpkin characterizations.

Ecological role and potential extinction of Amorphophallus variabilis in Central Java, Indonesia

“…Amorphophallus variabilis has a very high tolerance to shade so that the plant will grow optimally if it lives in a shady area. Similar to Amorphophallus titanium, which was reported to thrive below the forest canopy in the Sumatra rainforest (Arianto et al 2018). A. variabilis does not need a lot of light.…”

Estimating the Genetic Diversity of Oranges Citrus spp. in South Sulawesi, Indonesia, Using RAPD Markers

“…One drawback of RAPD markers is their low reproducibility [46]. However, this can be minimized by optimizing PCR conditions, testing the reproducibility of selected primers by repeating PCR amplifcation three times under the same amplifcation conditions [47], choosing suitable primers [48], and ensuring optimal extraction methods [49]. Reproducibility in RAPD refers to the extent to which the results of RAPD analysis can be consistently reproduced when performed by diferent laboratories or individuals.…”

“…Populations with high genetic diversity have the ability to withstand diseases and extreme climatic changes, allowing them to persist over multiple generations [23]. Te high genetic diversity in the red pomelo (M) population is likely infuenced by the larger population size compared to other locations [49]. Te high diversity may also be attributed to cross-pollination facilitated by pollinator agents, which play a signifcant role in successful fertilization.…”

The ecology of suweg (Amorphophallus paeoniifolius) in the Citanduy and Cimanuk Watersheds, West Java, Indonesia

“…This study exploratory surveys and intensive studies. An exploratory survey was conducted randomly through direct observation to record the distribution and population of suweg and the biophysical factors in the Citanduy and Cimanuk Watersheds (Budiman and Arisoesilaningsih 2012;Yuzammi et al 2014;Arianto et al 2018;Hafsah et al 2018;Sungkajanttranon et al 2018;Hidayat 2019). Intensive studies were carried out to explore more detailed data related to the ecology of suweg in certain areas determined purposively (Etikan et al 2016).…”