Genetic diversity of Artemisia populations in central and north Saudi Arabia based on morphological variation and RAPD polymorphism

Badr, Abdelfattah; El-Shazly, Hanaa H.; Helail, Nagah S.; Ghanim, Wafaa El

doi:10.1007/s00606-012-0597-5

Cited by 29 publications

(17 citation statements)

References 24 publications

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“…RAPD technique requires small amount of DNA, easy to perform and reveals dominant molecular markers of ultimate potentialities in several fields of plant science including systematics and evolution (Witkus et al, 1994). RAPD markers can be used in genetic diversity, cultivar identification and genetic relationships in soybean (Mienie et al, 1995;Chowdhery et al, 2001;Baranek et al, 2002;Barakat, 2004) and other different plants and populations (Al-Khalifa et al, 2005;Bhutta et al, 2006;El-Shazly and El-Metairi, 2006;Lin et al, 2009;Badr et al, 2012;Hoque and Hasan, 2012;Prasanthi et al, 2012). The RAPD method also provides useful evidence for gene mapping (Barua et al, 1993 ;Liu et al, 2000).…”

Section: Genetics and Biotechnologymentioning

confidence: 99%

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DETECTION OF GENETIC DIVERSITY IN DIFFERENT SOYBEAN (Glycine max L.) CULTIVARS USING SEED STORAGE PROTEIN PROFILES AND RAPD-PCR ANALYSIS

2017

Zagazig Journal of Agricultural Research

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and Giza 111) and to elucidate their genetic relationships. The results of protein banding pattern showed a low level of polymorphism, so cannot be used for complete discrimination among the five cultivars under study. However, the results of protein profiles could be considered as indicators for general protein model pattern for the studied soybean cultivars. On the other hand, RAPD-PCR profiles revealed high levels of polymorphism among the five cultivars. Four 10-mer arbitrary primers successfully generated reproducible polymorphic products. Both number and size of the amplified products varied considerably with different primers and a sum of 22 polymorphic and 9 monomorphic bands were generated in all cultivars that used. A total of 8 unique bands were also identified. Two of the primers were more successful in cultivar's identification since they produced unique bands that are characteristics of each cultivar under study. The combination of all polymorphic bands generated by the four primers, were enough to discriminate between the examined soybean cultivars. Various combinations of the three RAPD decamer oligonucleotides had been used in the single-primer to increase the potential of the PCR reaction. To elucidate the genetic relationship, a dendrogram was constructed using both SDS-PAGE and RAPD profiles. The resulting dendrogram revealed three main genetic clusters; the first cluster include Giza22, Crawford and Giza35, while the second cluster, comprised the cultivar Giza21, and the third cluster comprised the cultivar Giza111. The first group has been subdivided into two subgroups; the first subgroup comprised one cultivar (Giza35) whereas the second subgroup included the two cultivars Giza22 and Crawford.

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Section: Genetics and Biotechnologymentioning

confidence: 99%

“…RAPD-PCR markers have been used in different plants to assess variations. Badr et al (2012) reported the analysis of morphological variations and RAPD polymorphism distinguished among populations of Artenesia in Central and North Saudi Arabia.…”

Section: Rapd-pcr Analysismentioning

confidence: 99%

DETECTION OF GENETIC DIVERSITY IN DIFFERENT SOYBEAN (Glycine max L.) CULTIVARS USING SEED STORAGE PROTEIN PROFILES AND RAPD-PCR ANALYSIS

2017

Zagazig Journal of Agricultural Research

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“…Due to high number of species, ecological and cytological diversity coupled with economic importance, the genus Artemisia provides good source for specific and genetic diversified studies (Badr et al 2012). Due to highly suitable climatic conditions, the Western Himalayas is considered to be one of hot spot areas for Artemisia diversity.…”

Section: Introductionmentioning

confidence: 99%

Cytology of the genus Artemisia (Anthemidae, Asteraceae) in the Western Himalayas

Gupta¹,

Goyal²,

Singh³

2014

Biologia

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The present study revealed the varied frequency of natural chromosomal abnormalities in 13 populations pertaining to 9 species of the genus Artemisia L. from different localities of Himachal Pradesh (Western Himalaya). Intraspecific chromosome variability has been reported for the first time on worldwide basis in Artemisia vestita (2n = 2x = 36) and from India in A. macrocephala (2n = 2x = 18) and A. scoparia (2n = 2x = 36). Besides, B-chromosomes have been reported here for the first time in A. nilagirica and A. roxburghiana. Most of the populations show anomalous meiotic behaviour resulting in cytomixis, chromosomal stickiness, unoriented bivalents, formation of laggards and bridges which leads to abnormal microsporogenesis, and production of heterogeneous-sized fertile pollen grains along with reduced pollen fertility.

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“…The karyological markers developed allowed karyotypic differentiation between both species, uncovering peculiarities in the number and position of rDNA sites, which suggests chromosome rearrangements, such as translocations (Bernardes et al, 2013). Genetic variation within and between plant populations is essential for establishment the genetic diversity database of plant genetic resources (Badr et al, 2012;2014 and2016).…”

Section: Cotula Barbatamentioning

confidence: 99%

“…Detailed karyotype features of 23 species of subfamily Asteroideae were described by Badr et al (1997) and for 24 species from the tribes Chicorieae (Lactuceae) and Cardueae (Cynareae) by Badr et al (2009). Comparative karyological analysis was also described for selected species such as Artemisia judaica and Artemisia monosperma (Badr et al, 2012). Additional counts were added on Cotula barbata, Achillea fragrantissima, Achillea santolina, Anacyclus monanthos, Artemisia arborescens, Glebionis coronaria, Cotula cinerea and Matricaria recutita (Abd El Tawab et al, 2008;Badr et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Cytological Studies on Some Egyptian species of Anthemideae (Asteraceae)

et al. 2017

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THE MEIOTIC behavior and morphology of pollen grains of four species of Asteraceae (Glebionis coronaria, Anacyclus monanthos, Matricaria recutita and cotula barbata) were studied. Glebionis coronaria, Anacyclus monanthos and Matricaria recutita showed nine chromosome bivalents while those of Cotula barbata showed eight chromosome bivalents. The chromosomes were meiotically identified as two pairs in three taxa (Glebionis coronaria, Anacyclus monanthos and Matricaria recutita) while it was one pair in Cotulabarbata. The pollen shape and morphology were recognized by measuring polar and equatorial axis, Morphological characteristics of the pollen grains of the four studied species were considerably different.

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Genetic diversity of Artemisia populations in central and north Saudi Arabia based on morphological variation and RAPD polymorphism

Cited by 29 publications

References 24 publications

DETECTION OF GENETIC DIVERSITY IN DIFFERENT SOYBEAN (Glycine max L.) CULTIVARS USING SEED STORAGE PROTEIN PROFILES AND RAPD-PCR ANALYSIS

DETECTION OF GENETIC DIVERSITY IN DIFFERENT SOYBEAN (Glycine max L.) CULTIVARS USING SEED STORAGE PROTEIN PROFILES AND RAPD-PCR ANALYSIS

Cytology of the genus Artemisia (Anthemidae, Asteraceae) in the Western Himalayas

Cytological Studies on Some Egyptian species of Anthemideae (Asteraceae)

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