Genetic diversity of <i>Streptococcus suis</i> isolated from three pig farms of China obtained by acquiring antibiotic resistance genes

Huang, Jinhu; Shang, Ke; Kashif, Jam; Wang, Liping

doi:10.1002/jsfa.6841

Cited by 21 publications

(18 citation statements)

References 33 publications

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“…In total, a total of 163 STs were identified from the China and UK collections in this study, only 12 of which had been previously reported. In our study, the STs found among serotypes with strong potential of virulence (serotype 2 and 1/2 and serotypes 1 and 14) found in the Chinese collection were ST1, ST7, and ST11, similar to other published reports (8,16,30). This finding suggests that the S. suis isolates from healthy pigs are more diverse than those from diseased pigs.…”

Section: Discussionsupporting

confidence: 90%

Effects of Environmental and Management-Associated Factors on Prevalence and Diversity of Streptococcus suis in Clinically Healthy Pig Herds in China and the United Kingdom

Zou

Zhou

Xiao

et al. 2018

Appl Environ Microbiol

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, a global zoonosis of pigs, shows regional differences in the prevalence of human-associated disease for Asian and non-Asian countries. The isolation rates and diversities of on tonsils of healthy slaughter pigs in China and the United Kingdom were studied for effects of geography, temperature, pig age, and farm type. Isolates underwent analysis of molecular serotype and multilocus sequence type and virulence-associated genotyping. Although we found no significant difference in positive isolation rates between Chinese and UK farms, the prevalences of serotypes previously associated with human disease were significantly greater in the Chinese collection ( = 0.003). A significant effect of temperature was found on the positive isolation rate of the Chinese samples and the prevalence of human disease-associated serotypes in the UK population (China, = 0.004; United Kingdom, = 0.024) and on the prevalence of isolates carrying key virulence genes in China ( = 0.044). Finally, we found marked diversity among isolates, with statistically significant temperature effects on detection of multiple strain types within individual pigs. This study highlighted the high carriage prevalence and diversity of among clinically healthy pig herds of China and the United Kingdom. The significant effect of temperature on prevalence of isolation, human disease-associated serotypes, and diversity carried by individual pigs may shed new light on geographic variations in human -associated disease. is a global zoonotic pathogen and also a normal colonizer mainly carried on the tonsil of pigs. Thus, it is important to study the effect of environmental and management-associated factors on the populations in clinically healthy pigs. In this research, we investigated the similarities and differences between the populations obtained from different pig ages, seasons, and farm management systems and discovered the relationship between high climatic temperature and the prevalence of .

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Section: Discussionsupporting

confidence: 90%

Effects of Environmental and Management-Associated Factors on Prevalence and Diversity of Streptococcus suis in Clinically Healthy Pig Herds in China and the United Kingdom

Zou

Zhou

Xiao

et al. 2018

Appl Environ Microbiol

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“…Among S. suis isolates recovered from diseased ( n = 119) and healthy pigs ( n = 254) (Huang, Shang, Kashif, & Wang, ; Huang et al., ), thirty‐five isolates were unable to be assigned to 29 reference serotypes (Table S2). Twenty‐seven of 35 isolates were identified to Chz or NCLs (NCL1‐20), and the remaining isolates were non‐typeable by PCR assays, suggesting that these isolates carry unidentified NCLs.…”

Section: Resultsmentioning

confidence: 99%

Identification of six novel capsular polysaccharide loci ( NCL ) from Streptococcus suis multidrug resistant non‐typeable strains and the pathogenic characteristic of strains carrying new NCL s

Huang

Chen

et al. 2019

Transbound Emerg Dis

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Streptococcus suis is a major swine pathogen and an important zoonotic agent worldwide. At least nine serotypes can infect human so far. Although 29 serotypes (1–19, 21, 23–25, 27–31 and 1/2) strains are considered as authentic S. suis, a novel variant serotype Chz and strains carrying 20 novel capsular polysaccharide loci (NCL) have been identified recently. However, information about pathogenic and antimicrobial resistance characteristics of strains carrying NCLs is still unavailable. In this study, we identified six new NCLs (designated as NCL21‐26) from 35 non‐typeable S. suis strains by agglutination tests and whole genome sequencing analysis. Further analysis of the genetic context of NCL25 and NCL26 showed a mosaic structure of the capsular polysaccharide loci. NCL25 exhibited considerable similarity to that of serotypes 10 and 11, and NCL26 shared similarity to that of serotype 9 and NCL4. Antimicrobial susceptibility testing demonstrated that strains carrying NCL21‐26 were all resistant to clindamycin, lincomycin, erythromycin, tilmicosin and tetracycline. Animal infection experiments showed that the virulence of NCL26 strain NJ1112 isolated from a disease pig was similar to that of S. suis serotype 2 virulent strain SC070731 in both zebrafish and mouse infection models, highlighting the necessity for surveillance of strains belonging to NCL26. We also developed a multiplex PCR assay to detect NCL21‐26 strains. Our findings expand the views of genetic diversity of S. suis capsular polysaccharide loci and S. suis pathogenic characteristic.

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“…As a predominant resistant mechanism, the presence of ribosomal methylase genes ermA and/or ermB might be the reason causing highly resistant phenotype to erythromycin (Table S3 ), and, less frequently, harboring the efflux pump genes mefA and msrD (only in isolate 114) could mediate a medium level resistant to erythromycin. Although there are many genes mediating tetracycline resistance, the ribosomal protection genes of tetM and tetO were notably detectable in S. suis and a reduced frequency to efflux gene tetL (Roberts, 2005 ; Huang et al, 2015 ). In agreement with previous reports (Zhang et al, 2015 ), highly prevalence of tetM (8 of 11 isolates) and followed by tetO (three isolates) were detected in this study.…”

Section: Discussionmentioning

confidence: 99%

“…Tetracycline resistance in S. suis was found in North America, Asia, and many European countries ranging from 86.9% up to 100% in pigs (Seitz et al, 2016 ), and resistance in human meningitis cases was reported in Asia (Chu et al, 2009 ; Hoa et al, 2011 ). Extensive resistance has been reported against aminoglysides β-lactams, macrolide, lincosamide, trimethoprim, and amphenicols (Chu et al, 2009 ; Holden et al, 2009 ; Palmieri et al, 2011 ; Varela et al, 2013 ; Huang et al, 2015 ). Notably, the bacteria can be commensal or carried in various species, including birds, rabbits, cats, dogs, horses, cattle, fallow deer, and wild boars, which may cause widespread in nature and the persistence of endemic foci in pigs (Hernandez-Garcia et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Combination Therapy Strategies Against Multiple-Resistant Streptococcus Suis

Fang

Zheng

et al. 2018

Front. Pharmacol.

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Streptococcus suis is a major swine pathogen, an emerging zoonotic agent responsible for meningitis, endocarditis and septicaemia followed by deafness in humans. The development of antimicrobial resistance in S. suis increases the risk for therapeutic failure in both animals and humans. In this study, we report the synergism of combination therapy against multi-resistant S. suis isolates from swine. Twelve antibiotic profiles were determined against 11 S. suis strains. To investigate their synergistic/antagonistic activity, checkerboard assay was performed for all the possible combinations. In-vitro killing curves and in-vivo treatment trials were used to confirm the synergistic activity of special combinations against S. suis dominant clones. In this study, 11 S. suis isolates were highly resistant to erythromycin, clindamycin, trimethoprim/sulfamethoxazole, and tetracycline with ratios of 80–100%, and the resistance percentages to enrofloxacin, florfenicol, and spectinomycin were ~50%. The checkerboard data identified two combination regimens, ampicillin plus apramycin and tiamulin plus spectinomycin which gave the greatest level of synergism against the S. suis strains. In-vitro kill-curves showed a bacterial reduction of over 3-logCFU with the use of combination treatments, whilst the application of mono-therapies achieve less than a 2-logCFU cell killing. In-vivo models confirm that administration of these two combinations significantly reduced the number of bacterial cells after 24 h of treatment. In conclusions, the combinations of ampicillin plus apramycin and tiamulin plus spectinomycin showed the greatest synergism and may be potential strategies for treatment of multi-resistant S. suis in animal.

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Genetic diversity of Streptococcus suis isolated from three pig farms of China obtained by acquiring antibiotic resistance genes

Cited by 21 publications

References 33 publications

Effects of Environmental and Management-Associated Factors on Prevalence and Diversity of Streptococcus suis in Clinically Healthy Pig Herds in China and the United Kingdom

Effects of Environmental and Management-Associated Factors on Prevalence and Diversity of Streptococcus suis in Clinically Healthy Pig Herds in China and the United Kingdom

Identification of six novel capsular polysaccharide loci ( NCL ) from Streptococcus suis multidrug resistant non‐typeable strains and the pathogenic characteristic of strains carrying new NCL s

Combination Therapy Strategies Against Multiple-Resistant Streptococcus Suis

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