Genetic Diversity of Pathogenic and Nonpathogenic Populations of <i>Fusarium oxysporum</i> Isolated from Carnation Fields in Argentina

Lori, Gladys Albina; Edel-Hermann, Véronique; Gautheron, Nadine; Alabouvette, Claude

doi:10.1094/phyto.2004.94.6.661

Cited by 39 publications

(35 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Other studies indicate that F. oxysporum populations from soil are geographically heterogeneous and greatly influenced by resident plant populations (19,20,21), suggesting that some plants may exert a selective effect on soil populations of F. oxysporum (22). Studies comparing pathogenic and nonpathogenic F. oxysporum populations associated with the same host have found much higher levels of diversity in the nonpathogenic populations than in the pathogens (11,14,23,24).…”

mentioning

confidence: 99%

Highly Diverse Endophytic and Soil Fusarium oxysporum Populations Associated with Field-Grown Tomato Plants

Demers

Gugino

Jiménez-Gasco

2015

Appl Environ Microbiol

View full text Add to dashboard Cite

The diversity and genetic differentiation of populations of Fusarium oxysporum associated with tomato fields, both endophytes obtained from tomato plants and isolates obtained from soil surrounding the sampled plants, were investigated. A total of 609 isolates of F. oxysporum were obtained, 295 isolates from a total of 32 asymptomatic tomato plants in two fields and 314 isolates from eight soil cores sampled from the area surrounding the plants. Included in this total were 112 isolates from the stems of all 32 plants, a niche that has not been previously included in F. oxysporum population genetics studies. Isolates were characterized using the DNA sequence of the translation elongation factor 1␣ gene. A diverse population of 26 sequence types was found, although two sequence types represented nearly two-thirds of the isolates studied. The sequence types were placed in different phylogenetic clades within F. oxysporum, and endophytic isolates were not monophyletic. Multiple sequence types were found in all plants, with an average of 4.2 per plant. The population compositions differed between the two fields but not between soil samples within each field. A certain degree of differentiation was observed between populations associated with different tomato cultivars, suggesting that the host genotype may affect the composition of plant-associated F. oxysporum populations. No clear patterns of genetic differentiation were observed between endophyte populations and soil populations, suggesting a lack of specialization of endophytic isolates. Fusarium oxysporum is a well-known phytopathogenic fungus that affects hundreds of crops worldwide. Most studies of the fungus focus on the ability of F. oxysporum to cause vascular wilt on a particular host. However, environmental surveys of a wide range of habitats frequently find F. oxysporum in the absence of plant disease (1). These F. oxysporum populations, often referred to as nonpathogenic, although lack of pathogenicity is usually not confirmed, are a cosmopolitan component of soil communities. F. oxysporum can also infect and colonize plants asymptomatically as an endophyte. This endophytic lifestyle has not been well researched, despite reports of endophytic colonization by F. oxysporum on nearly 100 plant species (2).The interaction of endophytic F. oxysporum and plants may give insight into how pathogen recognition and plant resistance operate. In susceptible plants, pathogenic F. oxysporum penetrates the root, colonizes the root cortex, and then spreads through the xylem to the rest of the plant, causing occlusion of the xylem and ultimately wilting and death (3, 4). In host cultivars that are resistant to the pathogen, the spread of the fungus is blocked, and only limited colonization of host tissue occurs (5, 6, 7). Nonpathogenic F. oxysporum inoculated onto plants can colonize the root cortex similarly to pathogens, but host responses, such as thickening of cell walls and protuberances into intercellular spaces, seem to limit the growth of endophytes, similar to ...

show abstract

mentioning

confidence: 99%

Highly Diverse Endophytic and Soil Fusarium oxysporum Populations Associated with Field-Grown Tomato Plants

Demers

Gugino

Jiménez-Gasco

2015

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Vegetative compatibility has been used to classify isolates of F. oxysporum belonging to distinct formal specials (Klein et al 2005), races within a special form (Elena and Pappas 2006) and special forms and/or races within or between different geographical origins (Di Primo et al 2002;Pasquali et al 2005). VCG has been used to distinguish between nonpathogenic and pathogenic populations on the same host species (Lori et al 2004). In general, pathogenic isolates of F. oxysporum in the same VCG are assumed to be associated with the same clonal lineage, even if they are geographically isolated (Leslie 1993).…”

Section: Introductionmentioning

confidence: 99%

“…However, isolates belonging to different VCGs can also belong to the same clonal lineage (Baayen et al 2000). Several formae speciales of F. oxysporum have been characterized by VCG analysis (Lori et al 2004;Abo et al 2005), as well as by random amplified polymorphio DNA (RAPD) analysis (Alves-Santos et al 2007;Bayraktar et al 2008;Baysal et al 2010) and several studies have combined both markers (Vakalounakis et al 2004;Nagarajan et al 2006). This technique is appropriate for developing countries with inadequate facilities for molecular work and can rapidly determine genetic groups of many fungal pathogens and their relation to pathogenicity (Cumagun et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Relationship between pathogenicity, race and vegetative compatibility grouping among Algerian populations of Fusarium oxysporum f. sp. pisi causing pea wilt

Merzoug¹,

Belabid²

2018

Journal of Plant Protection Research

View full text Add to dashboard Cite

Fusarium oxysporum f. sp. pisi (FOP) is a significant and destructive pathogen of field pea in Algeria. In the present study, 50 isolates of F. oxysporum f. sp. pisi, the causal agent of pea (Pisum sativum) wilt, collected from different parts of western Algeria and representing four races of the pathogen, were analyzed for virulence. The wilt incidence ranged from 6.66 to 88.33% on a highly susceptible cultivar (Little Marvel). Twenty-one isolates belonging to four races of FOP and one nonpathogenic F. oxysporum (FO) isolate were analyzed for vegetative compatibility in order to reveal the genetic structure of the population and to check the reliability of the method for the identification of physiological races of FOP. Obtained results showed that the FOP isolates could be classified into four main vegetative compatibility groups (VCGs) that corresponded to races l, 2A, 2B and 5. The race 6 isolate fell into the race 1 VCG. To our knowledge, this is the first such study in Algeria of its kind.

show abstract

“…This was refined by Correll et al (1987). Since that time, numerous investigators have classified F. oxysporum isolated from various host plants into distinct VCGs using nit mutants (Elmer and Stephens 1989;Larkin et al 1990;Elias and Schneider 1991;Katan et al 1991;Loffler and Rumine 1991;Venter et al 1992;Fiely et al 1995;Woudt et al 1995;Woo et al 1996;Harveson and Rush 1997;Kondo et al 1997;Lori et al 2004;Vakalounakis et al 2005). Five VCGs of F. oxysporum f. sp.…”

mentioning

confidence: 99%

“…cepae from onion have been identified in Colorado, USA, and one of the VCGs was present in all three regions of Colorado (Swift et al 2002). The use of VCGs has also been performed to determine the diversity of nonpathogenic populations of F. oxysporum isolated from carnation fields in Argentina (Lori et al 2004). Beside for determining the VCGs of Fusarium, the use of nit mutant combining with selective media was also useful in studying population dynamics of pathogenic F. oxysporum f. sp.…”

mentioning

confidence: 99%

Vegetative Compatibility Groups within Fusarium oxysporum f. sp. cepae in Hokkaido-Japan

Widodo¹,

Kondo²,

Kobayashi³

et al. 2008

View full text Add to dashboard Cite

Genetic Diversity of Pathogenic and Nonpathogenic Populations of Fusarium oxysporum Isolated from Carnation Fields in Argentina

Cited by 39 publications

References 30 publications

Highly Diverse Endophytic and Soil Fusarium oxysporum Populations Associated with Field-Grown Tomato Plants

Highly Diverse Endophytic and Soil Fusarium oxysporum Populations Associated with Field-Grown Tomato Plants

Relationship between pathogenicity, race and vegetative compatibility grouping among Algerian populations of Fusarium oxysporum f. sp. pisi causing pea wilt

Vegetative Compatibility Groups within Fusarium oxysporum f. sp. cepae in Hokkaido-Japan

Contact Info

Product

Resources

About