Genetic Diversity of Peanut (Arachis hypogea L.) Cultivars as Revealed by RAPD Markers

Al-Saghir, Mohannad; Abdel‐Salam, Abdel‐Salam G.

doi:10.4236/ajps.2015.614233

Cited by 6 publications

(6 citation statements)

References 15 publications

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“…DNA was extracted from fresh leaves (oldest two leaves on the plant) of the mother plant and seven acclimatized plants (two or three plants every treatment) resulted from the best treatments of in vitro shoot multiplication (3 mg · L -1 BA + 1 mg · L -1 NAA, 1.5 mg · L -1 TDZ + 0.5 mg · L -1 NAA, and 1.5 mg · L -1 TDZ + 1 mg · L -1 NAA) by cetyltrimethylammonium bromide according to Doyle and Doyle (1990). Polymerase chain reaction (PCR) was performed and repeated three times using six random decamer primers (Table 1) (Al-Saghir and Abdel-Salam, 2015;Joshi et al, 2009). RAPD-PCR was carried out in presence of 1· Taq DNA polymerase buffer (10 mM Tris-HCl of pH 8.3, 50 mM KCl, 1.5 mM MgCl 2 ), 100 mM dNTPs, 5 pmol single random primers, 25 ng DNA template, and 0.5 unit of Taq DNA polymerase in a total volume of 25 mL.…”

Section: Methodsmentioning

confidence: 99%

Micropropagation and Genetic Fidelity of the Regenerants of Aglaonema ‘Valentine’ Using Randomly Amplified Polymorphic DNA

El-Mahrouk¹,

Dewir²,

Naidoo³

2016

horts

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The present study reports a simple protocol for in vitro regeneration of Aglaonema ‘Valentine’ using axillary shoot explants for rapid multiplication and production of true-to-type plants. Different concentrations of benzyladenine (BA; 0, 1, 3, 5, and 7 mg·L−1), kinetin (Kin; 0, 1, 3, 5, and 7 mg·L−1), thidiazuron (TDZ; 0, 0.5, 1.0, 1.5, and 2.0 mg·L−1), naphthalene acetic acid (NAA; 0, 0.5, and 1.0 mg·L−1), and indole-3-butyric acid (IBA; 0, 0.5, and 1.0 mg·L−1) were used for shoot regeneration. The highest shoot proliferation (5.0) was obtained on Murashige and Skoog (MS) medium supplemented with 1.5 mg·L−1 TDZ and 1 mg·L−1 NAA. In vitro rooting was easily achieved with 100% at all concentrations of NAA and IBA supplemented to half- or full-strength MS medium. Regenerated plantlets were acclimatized in greenhouse with 100% survival rate. Randomly amplified polymorphic DNA (RAPD) analysis confirmed the genetic fidelity of the regenerated plantlets and mother plant.

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Section: Methodsmentioning

confidence: 99%

Micropropagation and Genetic Fidelity of the Regenerants of Aglaonema ‘Valentine’ Using Randomly Amplified Polymorphic DNA

El-Mahrouk¹,

Dewir²,

Naidoo³

2016

horts

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“…These results agreed with Guo et al (2005) and Lang and Hang (2007) who reported that the distinctive RAPD patterns generated from peanut cultivars could be used as genomic fingerprint to establish the identity of a given genotype. Similarly, Al-Saghir and Abdel-Salam (2015) observed that the technique of RAPD could be used to detect the genetic diversity in peanut and give a successful fingerprinting of peanut using these markers. Lom and Rao (2015) indicated the efficacy of RAPD markers for detecting the genetic variability in the wild Musa acuminate.…”

Section: Rapd-pcr Analysismentioning

confidence: 96%

“…Application of RAPD markers in peanuts aid in determining the markers associated with genes controlling important traits. These molecular techniques assist in the identification of new and various sources of diversity which may aid breeders to choose what genotypes for creating new genetic combinations and to determine which genetic resources should be retained in a collection in order to conserve maximum genetic variation in the gene bank (Al-Saghir and Abdel-Salam 2015).…”

Section: Introductionmentioning

confidence: 99%

Assessment of growth and productivity of five peanut cultivars and genetic diversity using RAPD markers

2019

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Background: This study was conducted to evaluate the genetic diversity of five peanut cultivars grown under field conditions. A field experiment was conducted using five peanut cultivars (Giza-5, Giza-6, Ismailia-1, Gregory, and R92) in a randomized complete block design with five replications during two following seasons to estimate the performance of five peanut cultivars for vegetative growth, yield, and yield component traits as well as seed quality traits. Twenty RAPD primers were used to identify a unique fingerprint for each of five cultivars. Results: Giza-6 cultivar surpassed all the tested peanut cultivars in the most vegetative growth traits and yield and its components traits, while the lowest values were observed in Giza-5 cultivar. The dendrogram constructed from RAPD analysis showed that Gregory and Giza-5 were the most distant among five peanut cultivars. Conclusions: RAPD markers are useful in the detection of genetic diversity of peanut. The availability of genetic diversity is important for the genetic improvement of peanut.

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“…Breeders around the world have successfully developed hundreds of improved peanut cultivars by making use the diversity in peanut germplasm. The diversity has allowed breeders to better understand the evolutionary relationships among accessions, to sample germplasm in a more systematic fashion, and to develop strategies to incorporate useful diversity in their breeding programs (Al-Saghrir & Abdel-Salam, 2015).…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity Among Three Cultivars Of Peanut (Arachis hypogaea L.) Based On Rapd Markers

Suryadi

Yuniaty²,

Susanto³

2019

bioe

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Peanut (Arachis hypogea) is a typical plant species of tropical regions that has high economic value. The plantation is widely spread over many areas and the production is being pushed to meet the increasing demand. Peanut breeding program is aimed to improve genetic quality, mainly with resepct of production and thus information on genetic diversity is necessary as a basis for consideration in breeding, management and sustainable utilization. One approach to analyse genetic diversity of peanut is by using molecular markers. Random Amplified Polymorphic DNA (RAPD) is a widely used molecular marker for genetic diversity analysis. Therefore, the aim of this study was to assess genetic diversity of peanut cultivars, i.e. Jerapah, Kancil, and Hypoma 2, based on RAPD markers. The study was conducted in a survey method, in which three individuals of each cultivar were analyzed using PCR-RAPD technique employing twelve primers, i.e. OPA-1, OPA-2, OPA-9, OPA-13, OPB-2, OPB-3, OPB-4, OPB-5, OPB-7, OPB-11, OPB-12 and OPJ-07. Data analysis based on morphological data is also included. Molecular analysis revealed that only 7.55% polymorphic band was obtained, while most of the bands were monomorphic, indicating very low variation among the cultivars. The phenogram that constructed based on literature showed that Kancil was closer to Jerapah cultivar, while RAPD-based dendogram showed that Hypoma 2 was closer to Kancil cultivar.

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Genetic Diversity of Peanut (Arachis hypogea L.) Cultivars as Revealed by RAPD Markers

Cited by 6 publications

References 15 publications

Micropropagation and Genetic Fidelity of the Regenerants of Aglaonema ‘Valentine’ Using Randomly Amplified Polymorphic DNA

Micropropagation and Genetic Fidelity of the Regenerants of Aglaonema ‘Valentine’ Using Randomly Amplified Polymorphic DNA

Assessment of growth and productivity of five peanut cultivars and genetic diversity using RAPD markers

Genetic Diversity Among Three Cultivars Of Peanut (Arachis hypogaea L.) Based On Rapd Markers

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