Genetic Diversity of Pigeonpea (<i>Cajanus cajan</i> (L.) Millsp.) Cultivars and Its Wild Relatives Using Randomly Amplified Polymorphic DNA (RAPD) Markers

Abstract

Genetic diversity among and between 16 cultivars of pigeonpea (Cajanus cajan (L) Millsp.) and its wild relatives (C. albicans and C. lineatus) analysed using RAPD. Twenty two random primers with an average of 71.2% polymorphism produced 151 polymorphic bands. Cluster analysis based on these 151 RAPD markers revealed relatively low level (0.434 -0.714) of genetic diversity among cultivars and high level of diversity between cultivars and wild relatives. C. albicans and C. lineatus showed only 0.231 similarity with each other and C. albicans showed relatively higher similarity with C. cajan cultivars than that showed by C. lineatus. In dendrogram the 16 cultivars grouped into two distinct clusters comprising of seven and nine genotypes each while the wild species form out groups. Bootstrap analysis of the dendrogram was performed and resulted in significant bootstrap values. Principal components analysis (PCA) also revealed the similar results that of unweighted pair group method with arithmetic mean (UPGMA). The first, second and third PCs contributed 55.9%, 5.9%, and 5.6% of the variation, respectively, with cumulative variation of the first three PCs was 67.4%.

Genetic diversity of Palestine landraces of faba bean (Vicia faba) based on RAPD markers

“…Our results also revealed an average of 8.54 loci per primer (Table 3). A close average was also obtained with other legume crops such as pigeon pea using RAPD markers (Yadav et al, 2012). In addition, the maximum percentage of polymorphic markers was 71.4 (BC-298; Figure 1) and the minimum was 50.0 (OPA-05, -09, and -16).…”

“…However, only VF-22, VF-23, and VF-24 landraces, which were collected from the Bethlehem region were clustered together. Indeed, the similar agro-climate that characterizes the region may be the reason for showing more similarity with each other and therefore clustering together (Yadav et al, 2012). In addition, landraces of big seed sizes were also grouped together, which also implies high genetic similarities between them, and may belong to the same genotype.…”

Genetic Variations and Relationships of Papua’s Endemic Orchids Based on RAPD Markers

“…The highest GDs values were found between D. Stratiotes (Ac08) and D. anosnum (Ac14) with 0.90 GDs value then followed by pairwise differences of D. Stratiotes (Ac08) and Dendrobium sp3 with 0.77 GDs value and the smallest GDs value were found between D. Lineale type1 (2) and Dendrobium sp1 (Ac03) with 0.10 GDs value. The calculation results of genetic variation among orchid species in this investigation resemble those of Cajanus cajan, Cajanus albicans, and Cajanus liniatus which have a wide range of genetic differences based on RAPD markers [29].…”

Genetic Diversity among Pigeonpea (Cajanus cajan L. Millsp.) Genotypes Using Genic SSRs with Putative Function for Drought Tolerance

“…Discovery of molecular markers led to genetic diversity analysis using restriction fragment length polymorphism (Sivaramakrishnan et al, 2002), amplified fragment length polymorphism (Panguluri et al, 2006), random amplification of polymorphic DNA (Yadav et al, 2012), microsatellite markers and DArT (Yang et al, 2006). Nevertheless, the molecular basis of most agronomic traits in pigeonpea remains unexplored due to the low level of DNA polymorphism and limited number of validated molecular markers.…”

“…Genetic diversity is essential prerequisite in breeding for drought tolerance, increased yields, wider adaptation and desirable quality. Earlier studies on genetic diversity with limited number of genotypes has been reported in pigeonpea viz., 36 elite cultivated genotypes , 45 genotypes (Datta et al, 2013), 16 cultivars and 2 wild relatives (Yadav et al, 2012), 15 genotypes (Shende and Raut, 2013), 49 genotypes (Rekha et al, 2011), 88 accessions (Songok et al, 2010), 16 genotypes (Singh et al, 2008) and 14 genotypes (Chakraborty et al, 2013). These studies however, focused on studying overall genetic diversity among pigeonpea germplasm using genic and genomic SSRs not specifically SSR markers related to drought tolerance.…”