Genetic Diversity of Pleurotus pulmonarius Revealed by RAPD, ISSR, and SRAP Fingerprinting

Yin, Yonggang; Liu, Yu; Li, Huamin; Zhao, Shuang G.; Wang, Shouxian; Liu, Ying; Wu, Di; Xu, Feng

doi:10.1007/s00284-013-0489-0

Cited by 23 publications

(16 citation statements)

References 20 publications

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“…(BPSM10 and BPSM34) has been closely clustered together. This finding was similarly reported by Yin et al [20] and Ro et al[72] who reported that phenotypic characteristics of fruiting bodies of. P .…”

Section: Discussionsupporting

confidence: 88%

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Antimicrobial Potential, Identification and Phylogenetic Affiliation of Wild Mushrooms from Two Sub-Tropical Semi-Evergreen Indian Forest Ecosystems

Lallawmsanga¹,

Passari²,

Mishra³

et al. 2016

PLoS ONE

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The diversity of wild mushrooms was investigated from two protected forest areas in India and 231 mushroom specimens were morphologically identified. Among them, 76 isolates were screened for their antimicrobial potential against seven bacterial and fungal pathogens. Out of 76 isolates, 45 isolates which displayed significant antimicrobial activities were identified using ITS rRNA gene amplification and subsequently phylogenetically characterized using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. Sequencing of the ITS rRNA region classified the isolates into 16 genera belonging to 11 families. In total, 11 RAPD and 10 ISSR primers were selected to evaluate genetic diversity based on their banding profile produced. In total 337 RAPD and 312 ISSR bands were detected, among which percentage of polymorphism ranges from 34.2% to 78.8% and 38.6% to 92.4% by using RAPD and ISSR primers respectively. Unweighted Pair-Group Method with Arithmetic Mean (UPGMA) trees of selected two methods were structured similarly, grouping the 46 isolates into two clusters which clearly showed a significant genetic distance among the different strains of wild mushroom, with an similarity coefficient ranges from 0.58 to 1.00 and 0.59 to 1.00 with RAPD and ISSR analysis respectively. This reporthas highlighted both DTR and MNP forests provide a habitat for diverse macrofungal species, therefore having the potential to be used for the discovery of antimicrobials. The report has also demonstrated that both RAPD and ISSR could efficiently differentiate wild mushrooms and could thus be considered as efficient markers for surveying genetic diversity. Additionally, selected six wild edible mushroom strains (Schizophyllum commune BPSM01, Panusgiganteus BPSM27, Pleurotussp. BPSM34, Lentinussp. BPSM37, Pleurotusdjamor BPSM41 and Lentinula sp. BPSM45) were analysed for their nutritional (proteins, carbohydrates, fat and ash content), antioxidant potential. The present findings also suggested that the wild edible mushroom strains do not have only nutritional values but also can be used as an accessible source of natural antioxidants.

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Section: Discussionsupporting

confidence: 88%

“…In this study, we selected two DNA based markers like randomly amplified polymorphic DNA (RAPD), and inter-simple sequence repeats (ISSR) to understand the genetic diversity among the wild mushrooms as these marker systems have been routinely employed to assess genetic diversity [19,20]. …”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Potential, Identification and Phylogenetic Affiliation of Wild Mushrooms from Two Sub-Tropical Semi-Evergreen Indian Forest Ecosystems

Lallawmsanga¹,

Passari²,

Mishra³

et al. 2016

PLoS ONE

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show abstract

“…Cluster I were further separated in to two sub cluster (A and B) with 67.5% diversity. The result is confirmative with finding of Yin et al, (2013) who investigated that the molecular markers were used to analyze the genetic diversity of 15 Chinese P. pulmonarius cultivars. In total, 21 RAPD primers selected for generating data based on their clear banding profiles produced.…”

Section: Resultssupporting

confidence: 64%

Genetic Diversity Characterization of Pleurotus strains by Random Amplified Polymorphic DNA Fingerprinting

Yadav¹,

Chandra²,

Singh³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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“…The protocol is simple, efficient, and has a high production rate. It has been used successfully in genetic diversity analysis and construction of genetic maps of many plant species [14][15][16][17][18][19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity and Population Structure of Toona Ciliata Roem. Based on Sequence-Related Amplified Polymorphism (SRAP) Markers

Zhan

Que

et al. 2015

Forests

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Sequence-related amplified polymorphism (SRAP) markers were used to investigate the genetic diversity among 30 populations of Toona ciliata Roem. sampled from the species' distribution area in China. To analyze the polymorphism in the SRAP profiles, 1505 primer pairs were screened and 24 selected. A total of 656 SRAP bands ranging from 100 to 1500 bp were acquired, of these 505 bands (77%) were polymorphic. The polymorphism information content (PIC) values ranged from 0.32 to 0.45, with an average of 0.41. An analysis of molecular variance (AMOVA) indicated that the most significant variation was attributable to differences among the populations and that variation within the populations was OPEN ACCESSForests 2015, 6 1095 small. STRUCTURE analysis divided the 30 populations into two parts. The unweighted pair group method of arithmetic averages (UPGMA) clustering and principal coordinates analysis (PCoA) showed that the 30 populations could be classified into four types. The results demonstrate a clear geographical trend for T. ciliata in China and provide a theoretical basis for future breeding and conservation strategy of T. ciliata.

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Genetic Diversity of Pleurotus pulmonarius Revealed by RAPD, ISSR, and SRAP Fingerprinting

Cited by 23 publications

References 20 publications

Antimicrobial Potential, Identification and Phylogenetic Affiliation of Wild Mushrooms from Two Sub-Tropical Semi-Evergreen Indian Forest Ecosystems

Antimicrobial Potential, Identification and Phylogenetic Affiliation of Wild Mushrooms from Two Sub-Tropical Semi-Evergreen Indian Forest Ecosystems

Genetic Diversity Characterization of Pleurotus strains by Random Amplified Polymorphic DNA Fingerprinting

Genetic Diversity and Population Structure of Toona Ciliata Roem. Based on Sequence-Related Amplified Polymorphism (SRAP) Markers

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