Genetic diversity of rhizobia associated with Desmodium species grown in China

Abstract

Aims: Desmodia are leguminous plants used as important forage and herbal medicine in China. Little information is available about the nodule bacteria of Desmodium species. To understand the genetic diversity of rhizobia associated with Desmodium species grown in China, isolates from temperate and subtropical regions were obtained and analysed. Methods and Results: A total of 39 rhizobial strains isolated from 9 Desmodium species grown in China were characterized by PCR‐based 16S rDNA gene and 16S–23S rDNA intergenic spacer gene restriction fragment length polymorphism (RFLP) and 16S rRNA gene sequencing. The results showed high diversity among rhizobia symbiotic with Desmodium species. Most microsymbionts of Desmodium species belonged to Bradyrhizobium closely related to Bradyrhizobium elkanii, Bradyrhizobium japonicum and Bradyrhizobium yuanmingense. Several small groups or single strain were related to Rhizobium, Sinorhizobium or Mesorhizobium. Conclusions: Desmodium species formed nodules with diverse rhizobia in Chinese soils. Significance and Impact of the Study: These results offered the first systematic information about the microsymbionts of desmodia grown in the temperate and subtropical regions of China.

Genetic Diversity and Host Range of Rhizobia Nodulating Lotus tenuis in Typical Soils of the Salado River Basin (Argentina)

“…strain. Intriguingly, the L. tenuis symbionts were closely related to rhizobial species that have been shown to be common symbionts of Phaseolus vulgaris (25,40). Unfortunately, despite various attempts we were unable to amplify nodC or nifH gene sequences from Rhizobium symbionts of L. tenuis, which prevented us from obtaining further insight into the origin of their symbiotic capacities.…”

Genetic Diversity of Native Bradyrhizobia Isolated from Soybeans ( Glycine max L.) in Different Agricultural-Ecological-Climatic Regions of India

“…Since then, several studies have reported nodulation of various legumes by B. yuanmingense-related rhizobia (8,15,17,30). Studies of host specificity showed that these B. yuanmingense isolates were not capable of effective nodulation of soybean plants (8,17,31,33). Two representative B. yuanmingense soybean isolates were evaluated on soybean plants and were effective in symbiotic N 2 fixation (data not shown).…”

Bradyrhizobium iriomotensesp. nov., Isolated from a Tumor-Like Root of the LegumeEntada koshunensisfrom Iriomote Island in Japan

“…1,2) Throughout the years, scientists around the world have isolated a great number of bradyrhizobial strains from different climatic and geographical regions from a broad spectrum of host plants, such as Glycine max, 3) Parasponia, 4) Arachis hypogaea, 5) Lupinus, 6) Aeschynomene, 7) Beta vulgaris, 8) Adenocarpus, Chamaecytisus, Spartocytisus, 9) and Desmodium. 10) Diversity studies have been conducted to gain a better understanding of the taxonomy and distribution of bradyrhizobia, and to screen for strains that might be of use in agriculture and environmental management. An abundance of attention was directed towards the selection, identification, and development of new strains of bradyrhizobia with novel and useful characteristics.…”