Genetic diversity of sacha inchi accessions detected by AFLP molecular markers

Abstract

Sacha inchi (Plukenetia volubilis L.) is a species native to the Amazon region for which studies of genetic diversity are required to ensure the success in programs to breed cultivars for agriculture. The purpose of this study was to assess the genetic diversity among sacha inchi accessions using amplified fragment length polymorphism (AFLP) markers. Thirty-seven accessions of the sacha inchi germplasm bank of Embrapa Amazônia Ocidental were analyzed. The markers were identified with four primer combinations. The percentage of polymorphic loci was estimated and the similarity of accessions analyzed by calculating the arithmetic complement of the Jaccard coefficient and cluster analysis. The primers revealed 191 polymorphic loci. The parameters of the similarity values in class intervals were on average 0.739 and variance 0.01. The estimates of the number and percentage of the genetic distance values per class interval were highest in the range of 0.7 to 0.79, (248 and 37.24%, respectively); the highest percentage, 69.22%, corresponded to values of genetic distance above 0.7, showing mostly high genetic diversity among the accessions. The results indicated a geographic structure among accessions in relation to their origin, generating consistent and valuable data for breeding programs and species conservation.

Crop yield, genetic parameter estimation and selection of sacha inchi in central Amazon1

“…In Brazil, sacha inchi oil is produced by hand from plant materials with no breeding or detailed information about the chemical composition of seeds, and research has focused on in vitro propagation efficiency (Bordignon et al 2012, Rodrigues et al 2014 and seed germination traits (Oliveira et al 2013, Cardoso et al 2015 of the species. To date, there are few studies examining the cultivation and agronomic traits of genotypes prospected and cultivated under Brazilian conditions.…”

“…The use of in vitro cultivation has shown to be promising in the production of seedlings of the species (Bordignon et al 2012). Rodrigues et al (2014) observed an increasing yield and greater uniformity of in vitropropagated plants. These authors also reported that sacha inchi plants propagated in vitro had more evenly distributed flowering and started earlier than plants grown from seeds.…”

“…Overall, genotypes collected in the interior of the Amazonas state (accessions 1-25) were more promising in our evaluations, and these regions should be prioritized in future collections. Rodrigues et al (2013), in a study of the genetic diversity of these accessions by genetic markers, also identified a geographic structure among accessions, in relation to their origin.…”

Molecular genetic diversity and mating system in sacha inchi progenies1

“…In a study of the same species, Ocelák et al (2015), assessing 173 sacha inchi accessions using ISSR markers, observed an average of only eleven polymorphic fragments per primer combination. Also studying sacha inchi, Rodrigues et al (2013) obtained 191 polymorphic fragments using four AFLP primers combinations.…”

“…In Brazil, despite the increasing number of studies carried out on sacha inchi in recent years (Rodrigues et al 2013, Cardoso et al 2015, Valente et al 2017aand 2017b, the plant is still in the semiwild condition, although it has a great potential for development in the Amazon region. Research in several areas are needed in order improve the crop.…”