Genetic diversity of the D-genome in T. aestivum and Aegilops species using SSR markers

Abstract

Simple sequence repeats (SSRs), highly dispersed nucleotide sequences in genomes, were used for germplasm analysis and estimation of the genetic relationship of the D-genome among 52 accessions of T. aestivum (AABBDD), Ae. tauschii (D t D t ), Ae. cylindrica (CCD c D c ) and Ae. crassa (MMD cr1 D cr1 ), collected from 13 different sites in Iran. A set of 21 microsatellite primers, from various locations on the seven D-genome chromosomes, revealed a high level of polymorphism. A total of 273 alleles were detected across all four species and the number of alleles per each microsatellite marker varied from 3 to 27. The highest genetic diversity occurred in Ae. tauschii followed by Ae. crassa, and the genetic distance was the smallest between Ae. tauschii and Ae. cylindrica. Data obtained in this study supports the view that genetic variability in the D-genome of hexaploid wheat is less than in Ae. tauschii. The highest number of unique alleles was observed within Ae. crassa accessions, indicating this species as a great potential source of novel genes for bread wheat improvement. Knowledge of genetic diversity in Aegilops species provides different levels of information which is important in the management of germplasm resources.

Microsatellite analysis of genetic diversity and population genetic structure of Aegilops tauschii Coss. in Northern Iran

“…In previous morphological ) and SSR (Naghavi et al 2009) studies of Ae. tauschii, collected from some parts of Iran, a high genetic diversity was found.…”

Genetic diversity of wheat wild relatives using SSR markers

“…Ae. tauschii, as a progenitor of the D-genome of common wheat, is a potential donor of genetic variability for the improvement of common wheat (KHALIGHI et al, 2008;NAGHAVI et al, 2009;SCHNEIDER et al, 2008). Jointed grass (Ae.…”

Cloning and characterization of novel low molecular weight glutenin subunit genes from two Aegilops species with the C and D genomes

“…Aegilops spp., the wild related species to wheat, usually possesses beneficial characteristics such as high protein content and extensive glutenin variations, furthermore, they were considered as a reservoir of useful genes such as resistance to abiotic (drought, cold, heat and salt tolerances) and biotic (viral, bacterial, fungal and herbicide resistances) stresses, and increased photosynthetic yield Valkoun 2001;Xu et al 2004). Although LMW-GS have been extensively studied in Aegilops tauschii, which was the D genome donor of bread wheat (Gianibelli et al 2002;Hsam et al 2001;Naghavi et al 2009;Vensel et al 1997;Pflüger et al 2001;Zhao et al 2008), little progress has been made in cloning and characterizing of LMW-GS genes from more Aegilops species. In this study, four novel LMW glutenin genes from two wild Aegilops accessions were cloned and sequenced, and the complete coding sequences were obtained.…”