Genetic diversity of the west  European honey bee  (Apis mellifera mellifera  and A. m. iberica) II. Microsatellite loci

Garnery, Lionel; Franck, Pierre; Baudry, Emmanuelle; Vautrin, D.; Cornuet, Jean‐Marie; Solignac, Michel

doi:10.1051/gse:19980703

Cited by 26 publications

(45 citation statements)

References 10 publications

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“…However, since the mtDNA analyses of those samples did not reveal European haplotypes, any introgression is probably due to hybridization through mating rather than swarming of introduced colonies. This putative nuclear introgression may be tested with nuclear markers such the microsatellites that have been successfully used to assess introgression events between populations corresponding to different evolutionary lineages Garnery et al, 1998;De la Rúa et al, 2002;Jensen et al, 2005;Miguel et al, 2007), and also in honeybee populations subject to the Africanization process (Clarke et al, 2002;Kraus et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Apis mellifera evolutionary lineages in Northern Africa: Libya, where orient meets occident

et al. 2009

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The distribution of various evolutionary lineages of Apis mellifera subspecies in Africa is still controversial. We sampled honeybees from eight coastal locations and three Saharan oases in Libya and analyzed mtDNA variability with restriction fragment length polymorphisms and the sequence of the tRNAleu-cox2 intergenic region. Haplotypes belonging to the oriental O evolutionary lineage, including four which are newly described, were detected in all investigated locations. Haplotypes belonging to the European M lineage were rarely detected, probably reflecting the effect of sporadic importations. Honeybees belonging to the A lineage were detected in Al Aziziyah and Zlitan close to the Tunisian border. The distribution of the O lineage extends westward up to the border between Libya and Tunisia, a contact area between the O and A lineages. Various Libyan honeybee populations in Saharan oases are characterized by novel and unique haplotypes (O4, O5, O5 0 and O5 00 ). These might be natural relic populations that became isolated when the North African Sahara desert was still grassland (0.126-0.168 Myr ago).

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Section: Discussionmentioning

confidence: 99%

Apis mellifera evolutionary lineages in Northern Africa: Libya, where orient meets occident

et al. 2009

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“…A considerable recent research effort has focused on locating and characterising the remaining populations of native black bees in Europe Garnery et al 1998aGarnery et al , 1998bJensen et al 2005). Now that these efforts are becoming successful, maintaining stocks of native black bees becomes relevant.…”

Section: Reproductive Isolationmentioning

confidence: 98%

“…Honey bee mating is, contrary to other domesticated animals, very difficult to control, so that gene flow between honeybee subspecies is common Garnery et al 1998aGarnery et al , 1998b. This has resulted in hybridisation and introgression, or even the replacement of one subspecies by another.…”

Section: Introductionmentioning

confidence: 97%

“…Several new studies of honey bees in Europe have shown that most A. m. mellifera populations are threatened by hybridisation and introgression with other introduced honey bee subspecies (Garnery et al 1998a(Garnery et al , 1998bJensen et al 2005). For the conservation of remaining A. m. mellifera populations it is thus important to gain information on mating distances and isolation at a local scale.…”

Section: Introductionmentioning

confidence: 99%

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Quantifying honey bee mating range and isolation in semi-isolated valleys by DNA microsatellite paternity analysis

et al. 2005

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Honey bee males and queens mate in mid air and can fly many kilometres on their nuptial flights. The conservation of native honey bees, such as the European black bee (Apis mellifera mellifera), therefore, requires large isolated areas to prevent hybridisation with other subspecies, such as A. m. ligustica or A. m. carnica, which may have been introduced by beekeepers. This study used DNA microsatellite markers to determine the mating range of A. m. mellifera in two adjacent semi-isolated valleys (Edale and Hope Valley) in the Peak District National Park, England, in order to assess their suitability for native honey bee conservation and as isolated mating locations. Three apiaries were set up in each valley, each containing 12 colonies headed by a virgin queen and 2 queenright drone producing hives. The virgin queens were allowed to mate naturally with drones from the hives we had set up and with drones from hives owned by local beekeepers. After mating, samples of worker larvae were taken from the 41 queens that mated successfully and genotyped at 11 DNA microsatellite loci. Paternity analyses were then carried out to determine mating distances and isolation. An average of 10.2 fathers were detected among the 16 worker progeny. After correction for non-detection and non-sampling errors, the mean effective mating frequency of the test queens was estimated to be 17.2, which is a normal figure for honey bees. Ninety percent of the matings occurred within a distance of 7.5 km, and fifty percent within 2.5 km. The maximal mating distance recorded was 15 km. Queens and drones did occasionally mate across the borders between the two valleys, showing that the dividing mountain ridge Losehill does not provide complete isolation. Nevertheless, in the most isolated part of Edale sixty percent of all matings were to drones from Edale hives. The large majority of observed mating distances fell within the range of Hope Valley, making this site a suitable location for the long term conservation of a breeding population of black bees.

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“…Population analyses were performed on sub-samples with a set of six additional loci (A8, A88, A24, B124, A43, and A28). For the eleven loci, the primers and the PCR conditions have already been described (Estoup et al, 1994;Franck et al, 1999;Garnery et al, 1998). PCRs were conducted in a total volume of 10 µl including 2 µl of DNA extraction solution, 0.4 µM of each primer, 1.2 -1.7 mM MgCl 2 , 0.4 u of Taq polymerase (Promega), 1X reaction buffer, 200 ng of BSA, 1 µCi of a 33 P-dATP (Amersham), 75 µM dBTP, and 6 µM dATP.…”

Section: Dna Extraction and Polymerase Chain Reactionmentioning

confidence: 99%

Evolution of extreme polyandry: an estimate of mating frequency in two African honeybee subspecies, Apis mellifera monticola and A.m. scutellata

Franck¹,

Kœniger

Lahner

et al. 2000

Insectes soc.

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Mating frequencies in two related honeybee subspecies Apis mellifera monticola and A. m. scutellata were compared using a set of five microsatellite loci in order to discriminate among the major factors which have affected the evolution of extreme polyandry. We found that the queens of eight A. m. monticola colonies collected from an apiary in Malawi, mated 5 -19 times producing an average relatedness among workers of 0.328 ± 0.049. The six A. m. scutellata colonies collected from an apiary in South Africa displayed a significantly larger degree of polyandry, i.e. queens mated 10 -25 times and the genetic relatedness among nestmates was 0.283 ± 0.020. The difference in the degree of polyandry observed between the populations suggests that socially based selective pressures are not of primary importance in the evolution of extreme polyandry but that ecological selective pressures play a more significant role. However, exploring the relative contributions of these factors to the variation in levels of polyandry will require the use of colonies in which the level of polyandry is experimentally controlled.

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Genetic diversity of the west European honey bee (Apis mellifera mellifera and A. m. iberica) II. Microsatellite loci

Cited by 26 publications

References 10 publications

Apis mellifera evolutionary lineages in Northern Africa: Libya, where orient meets occident

Apis mellifera evolutionary lineages in Northern Africa: Libya, where orient meets occident

Quantifying honey bee mating range and isolation in semi-isolated valleys by DNA microsatellite paternity analysis

Evolution of extreme polyandry: an estimate of mating frequency in two African honeybee subspecies, Apis mellifera monticola and A.m. scutellata

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