Genetic diversity studies of coarse and fine rice using RAPD markers

Abstract

The availability of a genetically diverse gene pool is vitally important in varietal development. Molecular markers are being extensively utilized to explore the genetic diversity among native and exotic germplasm. This study was designed to reveal the genetic diversity and patterns of relationships among the 20 accessions/genotypes representative of basmati and non-basmati rice from the existing rice gene pool using RAPD markers. Employing RAPD, 17 decamer oligonucleotide primers directed the amplification of 116 fragments, out of which 101 were polymorphic (87.06%) while 15 fragments were monomorphic (12.93%). Similarity coefficients had ranged from 0.47 to 0.90. The average genetic similarity was calculated 0.68 (68%). In this study, the coarse rice genotypes showed more polymorphism (85.84%) than the fine rice genotypes (61.76%). Genotypes were clustered into 8 distinct groups: A, B, C, D, E, F, G, and H but two genotypes, i.e., Shadab and Kangni-27 showed divergence from all the genotypes of the groups. Therefore, these diverse genotypes may be included in future breeding programmes.

DNA barcoding of the tidal swamp rice (Oryza sativa) landraces from South Kalimantan, Indonesia

“…Several molecular markers have applicated to characterized the local rice germplasm, such as RAPD or random amplified polymorphic DNA (Arshad et al 2011), AFLP or amplified fragment length polymorphism 1594 (Mokuwa et al 2014, Rajkumar et al 2011, and SSR or microsatellite (Das et al 2013, Salgotra et al 2015, Sutoro et al 2015. However, these markers have also shown certain limitations, such as relatively inconsistent and subjective.…”

Genetic diversity of local durian (Durio zibethinus Murr.) cultivars of South Kalimantan’s province based on RAPD markers

“…Over past ten years, RAPD have been employed for determine the genetic diversity of various plants, i.e. rice [8,9], tomato [10], papaya [11], citrus [25], coconut [26], soybean [23], and durian [6,27,28].…”

GENETIC DIVERSITY AMONG SUGARCANE (Saccharum officinarum L.) GENOTYPES AS SHOWN BY RANDOMLY AMPLIFIED POLYMORPHIC DNA (RAPD)

“…RAPD markers had been used to study genetic diversity of various plants such as mango (Samal et al, 2012), Safflower (Amini et al, 2008), banana (Santos et al, 2010), Capsicum sp. (Thul et al, 2012), Jerusalem artichoke (Wangsomnuk et al, 2011), piper (Sen et al, 2010), Persea bombycina (Bhau et al, 2009), basil (Chen et al, 2013), rice (Arshad, et al, 2011), Hevea (Lam et al, 2009), Carica cubensis (Rodriguez et al, 2010), soybean (Al-Saghir and Salam, 2011), cowpea (Anatala et al, 2014) and physic nut (Rafii et al, 2012).…”