Genetic Effects of Povidone‐Iodine

Wlodkowski, Theodore J.; Speck, William T.; Rosenkranz, Herbert S.

doi:10.1002/jps.2600640726

Cited by 22 publications

(7 citation statements)

References 14 publications

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“…Thus, mutagenic activity of the agent in DNA polymerase-deficient Escherichia coli and in the Salmonella typhimurium test strain TA 1530, which detects base substitutions, has been reported [24][25][26]. However, it has been scrutinized whether bacterial assays may yield relevant data for this potent microbicide.…”

Section: Discussionmentioning

confidence: 99%

Virucidal and Chlamydicidal Activities of Eye Drops with Povidone-Iodine Liposome Complex

et al. 2000

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Povidone-iodine (PVP-I) is a broad-spectrum microbicide with in vitro activity against bacteria, viruses, fungi and protozoans. A 5% solution of PVP-I proved to be highly effective in ophthalmic surgery for the prophylaxis of endophthalmitis. For the antiseptic treatment of eye infections a novel application form of PVP-I has been developed by using a PVP-I liposome complex which demonstrated an excellent antimicrobial efficacy. In this study it could be shown that the novel liposomal formulations containing 2.5 or 5% PVP-I were as active as the aqueous solution against herpes simplex virus type 1, adenovirus type 8, coxsackievirus A9 and Chlamydia trachomatis in cell culture referring to equal PVP-I concentrations. Long-term cytotoxicity experiments demonstrated a moderate cytotoxicity for both formulations with a better tolerability of the liposomal PVP-I formulation compared with the aqueous solution. There is no evidence for a genotoxic activity of these agents.

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Section: Discussionmentioning

confidence: 99%

Virucidal and Chlamydicidal Activities of Eye Drops with Povidone-Iodine Liposome Complex

et al. 2000

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“…The genotoxicity published in earlier studies [1,2,3] seems to be a result of a cytotoxicityof >60%, and also the prolonged contact time with diploid cells may be responsible for detecting chromosome fragments.…”

Section: Discussionmentioning

confidence: 99%

“…Earlier in vitro studies demonstrated that DNA damage was produced by povidone-iodine as a result of an in situ modification of the deoxycytosine moiety of DNA to 5-iododesoxycytidine. In these studies, DNA was directly isolated from diploid cells and judged for shortening or for the presence of fragments [1,2,3]. In the meantime, new in vitro genotoxicity tests using mammalian cells were developed and recommended by OECD guidelines [4,5,6].…”

Section: Objectivementioning

confidence: 99%

Lack of in vitro Genotoxicity of Povidone-Iodine in Solution, in Ointment or in a Liposomal Formulation (Repithel<sup>®</sup>)

Müller¹,

Hai²,

Kramer³

2006

Dermatology

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The in vitro genotoxicity of povidone-iodine in Repithel^®, Betaisodona^® ointment and Betaisodona^® solution was investigated using CHO-K1 cells. The comet assay and chromosome aberration test were used to characterise the genotoxic potency of povidone-iodine within 4 h of contact with test cells. Methyl methanesulphonate without S9 activation and benzo[a]pyrene with S9 activation were used as positive controls. No higher concentrations than 6% Repithel, 5% Betaisodona ointment and 2% Betaisodona solution in culture medium with 10% fetal bovine serum were tested within 4 h of contact with CHO-K1 cells for the comet assay, because higher concentrations will provide >60% cytotoxicity. All tested concentrations of povidone-iodine formulations (4–6% Repithel, 3–5% Betaisodona ointment and 1–2% Betaisodona solution) are not genotoxic in the comet assay. 10% Repithel, 5% Betaisodona ointment and 2.5% Betaisodona solution in culture medium with 10% fetal bovine serum were final concentrations in the chromosome aberration test, which provide <60% cytotoxicity within 4 h of contact with CHO-K1 cells. All tested concentrations of povidone- iodine formulations (5–10% Repithel, 2.5–5% Betaisodona ointment and 1.25–2.5% Betaisodona solution) are not genotoxic in the chromosome aberration test. Using the comet assay and chromosome aberration test to characterise the genotoxic potency of povidone-iodine within 4 h of contact with CHO-K1 cells, there was no chromosome damage detected.

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“…Previous studies [24][25][26][27] on the muta genicity of antimicrobial agents have shown that some of these, although mutagenic, did not exhibit their mutagenic potential in the standard Salmonella mutagenicity assay. This is due to the fact that in the standard procedure results are expressed as mutants per plate (or mutants per bacteria added to the plate) rather than as mutants per survi vors.…”

Section: Resultsmentioning

confidence: 99%

“…For determination of mutagenicity in liquid medium, bacteria were exposed, as previously de scribed [24][25][26], to AgSu and at intervals, portions of the cultures are processed for determination of the total number of viable cells and revertants to histidine-independence by plating on complete (nutrient agar) and minimal medium containing a trace of histidine [21].…”

Section: Methodsmentioning

confidence: 99%