Genetic heterogeneity of the immunogenic viral capsid protein region of human parvovirus B19 isolates obtained from an outbreak in a pediatric ward

Takahashi, Naoko; Takada, Norio; Hashimoto, Takashi; Okamoto, Takashi

doi:10.1016/s0014-5793(99)00518-9

Cited by 8 publications

(6 citation statements)

References 26 publications

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“…It is noteworthy, that all the nucleotide differences observed between this new strain and the reference strains of genotype1 in nucleotide BLAST were synonymous as shown by alignment of sequence R in BLASTX. This further confirmed the lower variability of the VP1/VP2 region at the protein level than at the DNA level [ 27 ].…”

Section: Discussionsupporting

confidence: 72%

“…The corresponding virus isolates had identical partial VP1/VP2 DNA sequences and therefore appeared to represent a single B19 strain, thus pointing towards nosocomial transmission. Since nosocomial transmission of B19 infection is documented [ 23 , 27 ] and since the patients had no past transfusions, the presence of the same virus strain in the four patients can be interpreted as the result of nosocomial transmission. An alternative explanation would be that the detected B19 strain is a hitherto unknown variant circulating independently of hospital settings.…”

Section: Discussionmentioning

confidence: 99%

“…However, it does not exclude the possibility of nosocomial transmission which is strongly suggested by the epidemiological data. Sequencing of a larger part of the genome of the present isolates, especially in the more variable VP1 unique region [ 27 ] and further detection and analysis of other isolates from Tunisia would be needed to confirm virus transmission and strain identity.…”

Section: Discussionmentioning

confidence: 99%

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Parvovirus B19 infection in Tunisian patients with sickle-cell anemia and acute erythroblastopenia

Regaya

Oussaief

Béjaoui³

et al. 2007

BMC Infect Dis

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Background: Human parvovirus B19 is the etiologic agent of erythema infectiosum in children. It is also associated with other clinical manifestations in different target groups. Patients with chronic hemolytic anemia are at high risk of developing acute erythroblastopenia following infection by the virus. They usually become highly viremic and pose an increased risk of virus transmission. Close monitoring of such high risk groups is required for epidemiologic surveillance and disease prevention activities. Here we report a molecular epidemiological study on B19 virus infection in Tunisian patients with chronic hemolytic anemia.

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Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Parvovirus B19 infection in Tunisian patients with sickle-cell anemia and acute erythroblastopenia

Regaya

Oussaief

Béjaoui³

et al. 2007

BMC Infect Dis

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“…The first open reading frame encodes the nonstructural (NS1) protein, while the second encodes the capsid proteins VP1 and VP2, which are in frame and colinear with the exception of an additional 227 amino acids at the N terminus of VP1. Amino acid variability is high in the VP1 unique region (19), which is surface exposed and the target site of neutralizing antibodies (8,13).…”

mentioning

confidence: 99%

Phylogenetic Evidence for the Rapid Evolution of Human B19 Erythrovirus

Shackelton

Holmes

2006

J Virol

122

114

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Human B19 erythrovirus is a ubiquitous viral pathogen, commonly infecting individuals before adulthood. As with all autonomous parvoviruses, its small single-stranded DNA genome is replicated with host cell machinery. While the mechanism of parvovirus genome replication has been studied in detail, the rate at which B19 virus evolves is unknown. By inferring the phylogenetic history and evolutionary dynamics of temporally sampled B19 sequences, we observed a surprisingly high rate of evolutionary change, at approximately 10 ؊4 nucleotide substitutions per site per year. This rate is more typical of RNA viruses and suggests that high mutation rates are characteristic of the Parvoviridae.Human B19 erythrovirus was first discovered in the serum of healthy blood donors (3) and has since been detected worldwide. Infection usually occurs in childhood, through respiratory droplets, and by age 15 approximately 50% of children have antibodies to the virus. Most childhood infections are asymptomatic, with erythema infectiosum, probably caused by the formation of immune complexes, the most frequent complication. In adults, however, infection often results in arthropathy and in some cases causes transient aplastic crisis. In immunocompromised individuals a persistent infection usually occurs, resulting in red cell aplasia, while transplacental transmission can lead to miscarriage or hydrops fetalis (22).Human erythroviruses belong to the family Parvoviridae, whose single-stranded (ss) DNA genomes are among the smallest of all DNA viruses. B19 virus is ϳ5.5 kb in length with two major open reading frames flanked by inverted terminal repeats, part of which form hairpin stems for priming replication through a double-stranded (ds) intermediate (2). The first open reading frame encodes the nonstructural (NS1) protein, while the second encodes the capsid proteins VP1 and VP2, which are in frame and colinear with the exception of an additional 227 amino acids at the N terminus of VP1. Amino acid variability is high in the VP1 unique region (19), which is surface exposed and the target site of neutralizing antibodies (8, 13).Unlike large dsDNA viruses, all autonomous parvoviruses replicate with host cell machinery (10). A common assumption in studies of viral evolution is that DNA viruses have low rates of evolutionary change, near those of their hosts, as observed in the large dsDNA herpesviruses (7, 14) and the small dsDNA human papillomaviruses (1). However, it was recently observed that one group of small ssDNA viruses, the carnivore parvoviruses, have a rate of nucleotide substitution many orders of magnitude higher, at approximately 1 ϫ 10 Ϫ4 substitutions/site/year, that is within the range seen in RNA viruses (17). It is currently unclear whether this unexpectedly high rate is characteristic of the carnivore parvoviruses alone or whether it typifies the entire Parvoviridae family. To address this issue we estimated the rate of nucleotide substitution in the human virus B19, a distant relative of the carnivore parvoviruses. B...

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“…As described in the introduction, multiple genotypes of the parvovirus B19, HBoV and PARV4 have been found to date. In parvovirus B19 infection this genetic heterogeneity has not been linked to differences in clinical presentation [26,27]. In HBoV the different genotypes do give different clinical manifestations; respiratory symptoms in HBoV1 and gastrointestinal tract symptoms in HBoV2-4 [15].…”

Section: Clinical Manifestations and Viral Genotypesmentioning

confidence: 99%

Review: Pathogenesis of Parvovirus Infections in Children

Beers-Tas¹,

Heidema²

2013

Virology

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Parvoviral infections are associated with a wide variety of diseases. Several members of the parvoviridae family have been shown to infect humans. In this review we discuss the pathogenesis of these infections and the spectrum of clinical diseases they are associated with. Their role in deregulation of erythrogenoid progenitors, as well as their possible role in the development of autoimmune disease and acute lymphoblastic leukaemia is highlighted.

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Genetic heterogeneity of the immunogenic viral capsid protein region of human parvovirus B19 isolates obtained from an outbreak in a pediatric ward

Cited by 8 publications

References 26 publications

Parvovirus B19 infection in Tunisian patients with sickle-cell anemia and acute erythroblastopenia

Parvovirus B19 infection in Tunisian patients with sickle-cell anemia and acute erythroblastopenia

Phylogenetic Evidence for the Rapid Evolution of Human B19 Erythrovirus

Review: Pathogenesis of Parvovirus Infections in Children

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