Genetic instability at the S-locus of Lycopersicon peruvianum plants regenerated from in vitro culture of anthers: Generation of new S-specificities and S-allele reversions

Ramulu, K. Sree

doi:10.1038/hdy.1982.105

Cited by 30 publications

(4 citation statements)

References 33 publications

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“…Previous experimental observations have hinted that SI plants can generate new S alleles after inbreeding, which are sometimes able to revert to the original allele several generations later [20,21,26]. We have noted that the $11 allele contains no stop codons in its single intron, and suggest that a capacity to read through this intron may represent a novel mechanism for generating a new S allele which can revert back to its original form.…”

Section: Introductionmentioning

confidence: 60%

“…In our case, a new S protein translated from unspliced message would have a different HVa region and potentially produce a different phenotype. An S allele with these unusual properties would explain several previous observations on spontaneous generation of new S alleles in inbred plants of L. peruvianum produced by selfing [21] or regenerated through tissue culture [26]. These observations include (1) a sudden self-incompatibility behavior of the original inbred, with the first appearance of the new S alleles restricted to the pistils, (2) the subsequent recovery of the self-incompatibility phenotype in individuals carrying the new S allele and (3) the ability of some individuals stabilized for the new S allele to revert to the original S specificity [20] or to a very similar form [26].…”

mentioning

confidence: 74%

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The S11 and S13 self incompatibility alleles in Solanum chacoense Bitt. are remarkably similar

Saba-El-Leil¹,

Rivard²,

Morse³

et al. 1994

Plant Mol Biol

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A genomic clone of the S11 allele from the self-incompatibility locus (S locus) in Solanum chacoense Bitt. has been isolated by cross-hybridization to the S. chacoense S13 allele and sequenced. The sequence of the S11 allele contains all the features expected for S genes of the Solanaceae, and S11 expression, as assessed by northern blots and RNA-PCR, was similar to that of other S. chacoense S alleles. The S11 protein sequence shares 95% identity with the phenotypically distinct S13 protein of S. chacoense and is the gametophytic S allele with the highest similarity to an existing allele so far discovered. Only 10 amino acid changes differentiate the mature proteins from these two alleles, which sets a new lower limit to the number of changes that can produce an altered S allele specificity. The amino acid substitutions are not clustered, suggesting that an accumulation of random point mutations can generate S allele diversity. The S11 intron is unusual in that it could be translated in frame with the coding sequence, thus suggesting an additional mechanism for the generation of new S alleles.

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Section: Introductionmentioning

confidence: 60%

mentioning

confidence: 74%

The S11 and S13 self incompatibility alleles in Solanum chacoense Bitt. are remarkably similar

Saba-El-Leil¹,

Rivard²,

Morse³

et al. 1994

Plant Mol Biol

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“…In another experiment, anther cultures of a L. peruvianum clone yielded a new S allele (Ramulu, 1982). This allele exhibited specificities previously unknown in the comprehensive experience of the writer and of de N ettancourt et al (1975).…”

Section: Mutationmentioning

confidence: 83%

The Tomato Crop

Atherton¹,

Rudich²

1986

118

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“…These results integrate with numerous other results showing that androgenesis induces variability. For example, a high proportion of anther-derived regenerants carried sexually heritable changes, namely at the 5locus of Lycopersicon peruvianum (16). The double-haploids of Nicotiana sylvestris showed qualitative and quantitative variations in the nuclear DNA content (17).…”

Section: Discussionmentioning

confidence: 99%

Variations in the Leaf Alkaloid Content of Androgenic Diploid Plants ofDatura innoxia

Hérouart¹,

Sangwan²,

Fliniaux³

et al. 1988

Planta Med

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Anther culture of DATURA INNOXIA Mill, has permitted the obtention of spontaneous diploid androgenic plants which produced the tropane alkaloids. The source plants (zygotic diploid) showed no significant variations in the leaf alkaloid content. On the contrary, androgenic diploid plants obtained after the first cycle of androgenesis showed important quantitative and qualitative variations in the leaf alkaloid content. Thus, androgenesis was found to induce a large variation in the accumulation of these secondary metabolites in the leaves. It has also permitted the obtention of tropane alkaloid-overproductive plants, particularly rich in scopolamine. The analyses of zygotic plants obtained from seed germination of the first cycle androgenic plants have shown that this variability is transmissible by simple cross-pollination. The analyses of androgenic diploid plants obtained after the second cycle of androgenesis also showed variations in the leaf alkaloid content. IN VITRO androgenesis, therefore, clearly induced variability in the leaf alkaloid content of the androgenic plants. The role of IN VITRO androgenesis in inducing variability has been discussed.

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Genetic instability at the S-locus of Lycopersicon peruvianum plants regenerated from in vitro culture of anthers: Generation of new S-specificities and S-allele reversions

Cited by 30 publications

References 33 publications

The S11 and S13 self incompatibility alleles in Solanum chacoense Bitt. are remarkably similar

The S11 and S13 self incompatibility alleles in Solanum chacoense Bitt. are remarkably similar

The Tomato Crop

Variations in the Leaf Alkaloid Content of Androgenic Diploid Plants ofDatura innoxia

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