Genetic Manipulation of Calcium Release-Activated Calcium Channel 1 Modulates the Multipotency of Human Cartilage-Derived Mesenchymal Stem Cells

Liu, Shuang; Takahashi, Minae; Kiyoi, Takeshi; Toyama, Kensuke; Mogi, Masaki

doi:10.1155/2019/7510214

Cited by 9 publications

(4 citation statements)

References 31 publications

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“…[143,197,201] Since the spark of regenerative medicine fuelled the interest in controlling the repair process, the predominant approaches to achieve the phenotypic modulation of SCs have been: small molecules that target the intracellular pathways; genetically manipulating the cells; and engineering the bio-physical properties of the matrices. [281][282][283][284][285][286][287] In parallel, several types of smart dynamic biomaterials have also been developed to stimulate regenerative cells: [288,289] surfaces endowed with stimulireacting properties (such as photo-actionability, electro or thermo-responsiveness), and enzymatic sensitivity can both support cell self-renewal and differentiation with spatiotemporal control. [79,155,160,290,291] The possibility to regulate the tissue healing process at a clinical level by simply applying external MFs is an extremely appealing objective, and the body of literature concerning the role of magnetism in affecting cell behavior is continuously expanding.…”

Section: Discussion and Future Perspectivesmentioning

confidence: 99%

Engineered Magnetic Nanocomposites to Modulate Cellular Function

Filippi

Garello

Yaşa

et al. 2021

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Magnetic nanoparticles (MNPs) have various applications in biomedicine, including imaging, drug delivery and release, genetic modification, cell guidance, and patterning. By combining MNPs with polymers, magnetic nanocomposites (MNCs) with diverse morphologies (core–shell particles, matrix‐dispersed particles, microspheres, etc.) can be generated. These MNCs retain the ability of MNPs to be controlled remotely using external magnetic fields. While the effects of these biomaterials on the cell biology are still poorly understood, such information can help the biophysical modulation of various cellular functions, including proliferation, adhesion, and differentiation. After recalling the basic properties of MNPs and polymers, and describing their coassembly into nanocomposites, this review focuses on how polymeric MNCs can be used in several ways to affect cell behavior. A special emphasis is given to 3D cell culture models and transplantable grafts, which are used for regenerative medicine, underlining the impact of MNCs in regulating stem cell differentiation and engineering living tissues. Recent advances in the use of MNCs for tissue regeneration are critically discussed, particularly with regard to their prospective involvement in human therapy and in the construction of advanced functional materials such as magnetically operated biomedical robots.

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Section: Discussion and Future Perspectivesmentioning

confidence: 99%

Engineered Magnetic Nanocomposites to Modulate Cellular Function

Filippi

Garello

Yaşa

et al. 2021

Small

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“…The depletion of intracellular Ca 2+ stores causes subsequent store-operated Ca 2+ entry (SOCE) through CRACs consisting of Orai1, Orai2 and stromal interaction molecule 1 (STIM1) and causes membrane hyperpolarization [21,[73][74][75][76]. CRAC-modified mesenchymal stem cells (MSCs) have distinct differentiation fates to adipocytes, osteoblasts, and chondrocytes from multipotent mesenchymal stem cells [77].…”

Section: The Mechanosensory Processes In Chondrocytesmentioning

confidence: 99%

Mechanosensory and mechanotransductive processes mediated by ion channels in articular chondrocytes: Potential therapeutic targets for osteoarthritis

et al. 2021

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Articular cartilage consists of an extracellular matrix including many proteins as well as embedded chondrocytes. Articular cartilage formation and function are influenced by mechanical forces. Hind limb unloading or simulated microgravity causes articular cartilage loss, suggesting the importance of the healthy mechanical environment in articular cartilage homeostasis and implying a significant role of appropriate mechanical stimulation in articular cartilage degeneration. Mechanosensitive ion channels participate in regulating the metabolism of articular chondrocytes, including matrix protein production and extracellular matrix synthesis. Mechanical stimuli, including fluid shear stress, stretch, compression and cell swelling and decreased mechanical conditions (such as simulated microgravity) can alter the membrane potential and regulate the metabolism of articular chondrocytes via transmembrane ion channel-induced ionic fluxes. This process includes Ca 2+ influx and the resulting mobilization of Ca 2+ that is due to massive released Ca 2+ from stores, intracellular cation efflux and extracellular cation influx. This review brings together published information on mechanosensitive ion channels, such as stretch-activated channels (SACs), voltage-gated Ca 2+ channels (VGCCs), large conductance Ca 2+ -activated K + channels (BK Ca channels), Ca 2+ -activated K + channels (SK Ca channels), voltage-activated H + channels (VAHCs), acid sensing ion channels (ASICs), transient receptor potential (TRP) family channels, and piezo1/2 channels. Data based on epithelial sodium channels (ENaCs), purinergic receptors and N-methyl-d-aspartate (NMDA) receptors are also included. These channels mediate mechanoelectrical physiological processes essential for converting physical force signals into biological signals. The primary channel-mediated effects and signaling pathways regulated by these mechanosensitive ion channels can influence the progression of osteoarthritis during the mechanosensory and mechanoadaptive process of articular chondrocytes.

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“…Additionally, Liu et al [ 68 ] showed that overexpression of Orai1 promoted human cartilage-derived MSC differentiation to osteoblasts. Lee et al [ 69 ] reported on the Orai1 contribution to osteogenic effects of BMP2.…”

Section: Ca 2+ -Permeable Channel Superfamilies In Osteoblast Lineagesmentioning

confidence: 99%

Role of K+ and Ca2+-Permeable Channels in Osteoblast Functions

Kurihara

Ohya

2021

IJMS

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Bone-forming cells or osteoblasts play an important role in bone modeling and remodeling processes. Osteoblast differentiation or osteoblastogenesis is orchestrated by multiple intracellular signaling pathways (e.g., bone morphogenetic proteins (BMP) and Wnt signaling pathways) and is modulated by the extracellular environment (e.g., parathyroid hormone (PTH), vitamin D, transforming growth factor β (TGF-β), and integrins). The regulation of bone homeostasis depends on the proper differentiation and function of osteoblast lineage cells from osteogenic precursors to osteocytes. Intracellular Ca2+ signaling relies on the control of numerous processes in osteoblast lineage cells, including cell growth, differentiation, migration, and gene expression. In addition, hyperpolarization via the activation of K+ channels indirectly promotes Ca2+ signaling in osteoblast lineage cells. An improved understanding of the fundamental physiological and pathophysiological processes in bone homeostasis requires detailed investigations of osteoblast lineage cells. This review summarizes the current knowledge on the functional impacts of K+ channels and Ca2+-permeable channels, which critically regulate Ca2+ signaling in osteoblast lineage cells to maintain bone homeostasis.

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Genetic Manipulation of Calcium Release-Activated Calcium Channel 1 Modulates the Multipotency of Human Cartilage-Derived Mesenchymal Stem Cells

Cited by 9 publications

References 31 publications

Engineered Magnetic Nanocomposites to Modulate Cellular Function

Engineered Magnetic Nanocomposites to Modulate Cellular Function

Mechanosensory and mechanotransductive processes mediated by ion channels in articular chondrocytes: Potential therapeutic targets for osteoarthritis

Role of K+ and Ca2+-Permeable Channels in Osteoblast Functions

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