Genetic Mapping of the Tph1 Gene Controlling Beta-tocopherol Accumulation in Sunflower Seeds

Vera-Ruiz, Elsa M.; Velasco, Leonardo; Leόn, Alberto J.; Fernández‐Martínez, José M.; Pérez‐Vich, Begoña

doi:10.1007/s11032-005-5678-5

Cited by 26 publications

(18 citation statements)

References 18 publications

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“…However, despite comprehensive research in this field, the contribution of individual tocopherol analogs to the oxidative stability of vegetable oils stored under household conditions has not been fully elucidated yet. Among the tocopherols, β-tocopherol was not analyzed because of its low abundance in the oil types tested [ 12 , 29 , 30 ]. Previous studies have shown that the concentrations of β-tocopherol in the tested oil types were below the limit of detection (0.2 ± 0.02 µg/mL) or the limit of quantification (0.67 ± 0.07 µg/mL) [ 29 , 31 , 32 ].…”

Section: Discussionmentioning

confidence: 99%

Contribution of the Ratio of Tocopherol Homologs to the Oxidative Stability of Commercial Vegetable Oils

et al. 2018

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The antioxidant activity of tocopherols in vegetable oils was shown to chiefly depend on the amount and the tocopherol homolog present. However, the most effective ratio of tocopherol homologs with regard to the antioxidant capacity has not been elucidated so far. The present study analyzed the effect of different tocopherol concentrations, homologs and ratios of homologs on markers of lipid oxidation in the most commonly consumed vegetable oils (canola, sunflower, soybean oil) stored in a 12 h light/dark cycle at 22 ± 2 °C for 56 days under retail/household conditions. After 56 days of storage, the α-tocopherol-rich canola and sunflower oil showed the strongest rise in lipid peroxides, yielding 25.1 ± 0.03 meq O2/kg (+25.3-fold) and 24.7 ± 0.05 meq O2/kg (+25.0-fold), respectively. ESR experiments, excluding effects of the oils’ matrices and other minor constituents, confirmed that a food representative tocopherol ratio of (γ + δ)/α = 4.77, as represented in soybean oil, led to a more pronounced delay of lipid oxidation than a lower ratio in canola (1.39) and sunflower oil (0.06). An optimum (γ + δ)/α -tocopherol ratio contributing to the oxidative quality of vegetable oils extending their shelf life has to be investigated.

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Section: Discussionmentioning

confidence: 99%

Contribution of the Ratio of Tocopherol Homologs to the Oxidative Stability of Commercial Vegetable Oils

et al. 2018

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“…Biparental populations based on crosses between elite breeding, conventional, or introgressed lines (e.g., Berry et al, 1995 ; Horn et al, 2003 ; Vera-Ruiz et al, 2006 ; Kane et al, 2013 ; Livaja et al, 2016 ) as well as landraces and wild species (e.g., Quillet et al, 1995 ; Kim and Rieseberg, 1999 ; Brouillette et al, 2007 ; Ma et al, 2017 ) have been employed in sunflower for mapping of genes, marker detection, QTL analyses and gene cloning. In addition, recombinant inbred lines (RILs) have been developed that as immortals can be maintained forever by self-propagation (e.g., Berrios et al, 1999 ; Tang et al, 2002 ; Tang et al, 2006 ; Poormohammad Kiani et al, 2007a ; Talukder et al, 2016 ).…”

Section: Plant Genetic Resources In Sunflowermentioning

confidence: 99%

Sunflower Hybrid Breeding: From Markers to Genomic Selection

Dimitrijević

Horn

2018

Front. Plant Sci.

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In sunflower, molecular markers for simple traits as, e.g., fertility restoration, high oleic acid content, herbicide tolerance or resistances to Plasmopara halstedii, Puccinia helianthi, or Orobanche cumana have been successfully used in marker-assisted breeding programs for years. However, agronomically important complex quantitative traits like yield, heterosis, drought tolerance, oil content or selection for disease resistance, e.g., against Sclerotinia sclerotiorum have been challenging and will require genome-wide approaches. Plant genetic resources for sunflower are being collected and conserved worldwide that represent valuable resources to study complex traits. Sunflower association panels provide the basis for genome-wide association studies, overcoming disadvantages of biparental populations. Advances in technologies and the availability of the sunflower genome sequence made novel approaches on the whole genome level possible. Genotype-by-sequencing, and whole genome sequencing based on next generation sequencing technologies facilitated the production of large amounts of SNP markers for high density maps as well as SNP arrays and allowed genome-wide association studies and genomic selection in sunflower. Genome wide or candidate gene based association studies have been performed for traits like branching, flowering time, resistance to Sclerotinia head and stalk rot. First steps in genomic selection with regard to hybrid performance and hybrid oil content have shown that genomic selection can successfully address complex quantitative traits in sunflower and will help to speed up sunflower breeding programs in the future. To make sunflower more competitive toward other oil crops higher levels of resistance against pathogens and better yield performance are required. In addition, optimizing plant architecture toward a more complex growth type for higher plant densities has the potential to considerably increase yields per hectare. Integrative approaches combining omic technologies (genomics, transcriptomics, proteomics, metabolomics and phenomics) using bioinformatic tools will facilitate the identification of target genes and markers for complex traits and will give a better insight into the mechanisms behind the traits.

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“…Co-segregation of FAD2-1 with Ol in high-oleic sunflower mutant was also reported (Schuppert et al 2006). Three loci, Tph1 (m), Tph2 (g) and d, can control the level of a tocopherol in sunflower seed Tang et al 2006;Vera-Ruiz et al 2006). The amount of b tocopherol is increased by d locus in mutant inbred lines (m m) whereas the level of c tocopherol is enhanced by g locus in mutant inbred lines (g g) as a result of knockout of c tocopherol methyl transferase ).…”

Section: Introductionmentioning

confidence: 81%

Genetic variability of seed-quality traits in gamma-induced mutants of sunflower (Helianthus annuus L.) under water-stressed condition

et al. 2010

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Sunflower is one of the major annual world crops grown for edible oil and its meal is a potential source of protein for human consumption. It contains tocopherol that decreases potential risk of chronic diseases in human. The objectives of the current research are to assess the genetic variability and to identify AFLP markers and candidate genes associated with seed-quality traits under well-irrigated and water-stressed conditions in gammainduced mutants of sunflower. Two mutant lines, M8-826-2-1 and M8-39-2-1, with significant increased level of oleic acid were identified that can be used in breeding programs for quality increase high oxidative stability and heart-healthy properties.The significant increased level of tocopherol in mutant lines, M8-862-1N1 and M8-641-2-1, is justified by observed polymorphism for tocopherol pathway-related gene; MCT. The most important marker for total tocopherol content is E33M50_16 which explains 33.9% of phenotypic variance. One of the most important candidate genes involving fatty acid biosynthesis, FAD2 (FAD2-1), is linked to oleic and linoleic acids content and explained more than 53% of phenotypic variance. Common markers associated with different seed-quality traits in well-irrigated and water-stressed conditions could be used for markerassisted selection (MAS) in both conditions. Other markers, which are specific for one condition whereas linked to different traits or specific for a trait, could be useful for a given water treatment.

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Genetic Mapping of the Tph1 Gene Controlling Beta-tocopherol Accumulation in Sunflower Seeds

Cited by 26 publications

References 18 publications

Contribution of the Ratio of Tocopherol Homologs to the Oxidative Stability of Commercial Vegetable Oils

Contribution of the Ratio of Tocopherol Homologs to the Oxidative Stability of Commercial Vegetable Oils

Sunflower Hybrid Breeding: From Markers to Genomic Selection

Genetic variability of seed-quality traits in gamma-induced mutants of sunflower (Helianthus annuus L.) under water-stressed condition

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