Genetic modification of the marine‐derived yeast Yarrowia lipolytica with high‐protein content using a GPI‐anchor‐fusion expression system

Abstract

Yarrowia lipolytica SWJ-1b isolated from the marine fish gut was found to contain 47.6 g of crude protein per 100 g of cell dry weight and had potential use as single cell protein. When the gene encoding enhanced green fluorescent protein (EGFP) was inserted into the surface display plasmid pINA1317-YlCWP110 and expressed in uracil mutant of Y. lipolytica SWJ-1b, the corresponding protein was successfully displayed on the cell surface, and 100% of the yeast cells exhibited the anchored target proteins. We found that yeast cells displaying EGFP were similar to those of Y. lipolytica SWJ-1b. Furthermore, C(18:1) and C(18:3) fatty acids biosynthesis in the marine yeast cells displaying the heterologous EGFP was weakly enhanced compared with that in its wild-type. The results suggest that the marine-derived Y. lipolytica SWJ-1b can be armed with the heterologous protein by the genetic modification and further used as single cell protein.

Expression of the inulinase gene from the marine‐derived Pichia guilliermondii in Saccharomyces sp. W0 and ethanol production from inulin

“…The uracil mutant of Saccharomyces sp. W0 was isolated as described by Wang and colleagues (2009). One of the mutants was named Saccharomyces sp.…”

Effect of Nitrogen Concentration on the Biosynthesis of Citric Acid, Protein, and Lipids in the Yeast Yarrowia lipolytica

“…There is little information about the ability of citrate-producing strains of Y. lipolytica to synthesize protein in significant amounts. The highest protein production (up to 0.55 g / g DCW) has been reported for the marine strain Y. lipolytica SWJ-1b grown on glucose [ 39 ]. Suitable results were obtained in experiments on the protein synthesis by Y. lipolytica cultivated on the glucose-containing hydrolysates of rye and oats straw and bran (protein content consisted of 0.305–0.445 g / g DCW) [ 40 ], as well as during cultivation on glycerol-containing waste (up to 0.45 g / g DCW) [ 41 ].…”

Development and Prospect of Microbial Oils

“…Microbial can mostly synthetic a small amount of oil, but oil accumulation More than 20% of the parts can be called oleaginous microorganisms, such as Cryptococcus albidus, Lipomyces lipofera, Lipomyces starkeyi,Rhodotorula glutinis, Trichosporon pullulan, Rhodotorula mucilaginosa Cryptococcus curvatus, and Yarrowia lipolytica. The microorganisms of microbial oil have many advantages: the first cell growth is very rapid, growth cycle of cells is short, it can use a large of raw material sources or can use some of the low price of agricultural products in the cultivation of microorganisms are also beneficial to the environmental protection; microbial oil production can be artificial efficient regulation, little labor force, which is not influenced by the season, location, environment change [16][17][18][19][20] .…”