Genetic polymorphisms of leptin and leptin receptor genes in relation with production and reproduction traits in cattle.

Abstract

Leptin and leptin receptor genes are considered as production traits markers in dairy or beef cattle. The aim of this study was to verify the associations of polymorphisms in bovine LEP and LEPR genes with production and reproduction traits in Slovak Spotted and Pinzgau cows. Long-life production was evaluated: milk, protein, and fat yield and reproduction traits: age at first calving, calving interval, days open, and insemination interval. In total, 296 blood samples of Slovak Spotted and 85 hair roots samples of Pinzgau cows were analyzed. In order to detect LEP/Sau3AI (BTA 4, inron 2) and LEPR/T945M (BTA 3, exon 20) genotypes PCR-RFLP method was used. In Slovak Spotted and Pinzgau cows allele frequencies were 0.838/0.162 and 0.694/0.306 for A and B LEP variants, and 0.954/0.046 and 0.912/0.088 for C and T LEPR variants, respectively. For testing the associations between SNPs LEP/Sau3AI and LEPR/T945M and evaluated traits, the General Linear Model procedure in SAS Software was used. Statistical analysis showed that SNP LEP/Sau3AI significantly affected milk, protein and fat yield (P

Exploring the relationship between polymorphisms of leptin and IGF-1 genes with milk yield in indicine and taurine crossbred cows

“…GH-TaqI, LEP-Sau3AI and MYF5-TaqI polymorphisms of the genes investigated had significant effects, especially on the 305-day milk yield of Holstein cows (Sahin and Akyuz, 2017, Kiyici et al , 2019). Trakovicka, (2013) reported that the SNP LEP/Sau3AI significantly influence milk, protein and fat yield (P<0.05) in cows. Similarly polymorphism with respect to leptin gene was highly significant with milk yield and milk composition traits in Polish Black and White cows (Polish Friesian) (Flisikowsk et al , 2004).…”

R159Q Polymorphism in Leptin Gene and Its Correlation with Semen Quality Parameters in Nili-Ravi Buffalo Bulls

“…In another study, LEP/Sau3AI polymorphism and its effects on age at first calving, calving interval, days open, and insemination interval in Pinzgau and Slovak Spotted cows were RESEARCH ARTICLE monitored. It was noted that LEP/Sau3AI polymorphism significantly (P<0.01) affected the age at first calving in these cows (Trakovická et al, 2013).…”

Leptin Gene as Potential Gene for Molecular Selection on Cattle in Indonesia

“…Beberapa penelitian melaporkan secara khusus pada bagian promotor gen leptin sapi terdapat SNP berdasarkan GenBank: AJ571671 (Matteis et al 2012) dan AB070368 (Chung et al 2008). Polimorfisme gen leptin pada beberapa bangsa sapi dilaporkan memiliki asosiasi terhadap produksi karkas (Corva et al 2009), berat badan (Nobari et al 2010) dan produksi susu (Trakovická et al 2013). Studi tentang identifikasi SNP pada gen leptin sapi lokal di Indonesia masih sangat terbatas.…”

“…Respon energi yang meningkat menyebabkan terjadinya penghancuran sel trigliserida dalam jaringan lemak sebagai upaya untuk melepaskan asam lemak bebas yang digunakan untuk proses oksidasi dalam metabolisme tubuh (Denver et al 2011 (Moussavi et al 2006), Sistani (Nobari et al 2010), Sarabi (Javanmard et al 2008), Golpayegani, Taleshi (Nahavandi et al 2010), Mazandarani, Dashtiyari (Javanmard et al 2005), Fries Holstein (Liefers et al 2002), lokal Irak (Hussain et al 2017), Slovak Spotted dan Pinzgau (Trakovická et al 2013). Polimorfisme pada bagian ekson 2 gen leptin (Lep/Kpn2I) dilaporkan terjadi pada sapi Golpayegani, Taleshi, Sarabi, Sistani, Fries Holstein dan Brown Swiss (Nassiry et al 2008), Iranian Holstein (Sadeghi et al 2008), Frieswal, Ongole, Sahiwal (Singh et al 2014) dan Polish Holstein-Friesian (Komisarek 2010;Moravcikova et al 2012), Hanwoo (Kong et al 2006), Jersey, Hariana, Sahiwal, Gir dan Nimari (Choudhary et al 2005).…”

“…Polimorfisme pada bagian promotor gen leptin dilaporkan berasosiasi terhadap berat badan dan berat karkas (Chung et al 2008), kadar protein susu (Matteis et al 2012), tebal lemak punggung (Nkrumah et al 2005), luas mata rusuk/rib eye area (Corva et al 2009). Polimorfisme gen leptin pada bagian intron 2 dilaporkan berasosiasi terhadap produksi susu (Nobari et al 2010;Moravcikova et al 2012;Trakovická et al 2013), berat badan (Almeida et al 2003;Nobari et al 2010), berat karkas (Oprządek et al 2003), tebal lemak punggung (Curi et al 2011) dan masa kosong (Nobari et al 2010). Hasil yang berbeda dilaporkan oleh Hussain et al (2017) Polimorfisme gen leptin pada bagian ekson 2 dilaporkan berasosiasi terhadap berat karkas (Cheong et al 2006), lingkar dada (Kaygisiz et al 2011), tebal lemak punggung (Nkrumah et al 2004;Kong et al 2006), produksi susu (Sadeghi et al 2008) dan feed intake (Lagonigro et al 2003).…”