Genetic Relationships among Cultivated Diploid Plums and Their Progenitors as Determined by RAPD Markers

Most Japanese plum-type cultivars are self-incompatible and cross pollination is necessary to ensure fruit set. In this study, the S-RNase genotype and the incompatibility group of 68 Japanese plum-type cultivars were determined by PCR amplification of the S-RNase gene. The S-RNase genotype of 50 cultivars is first reported here and five new Japanese plum S-RNase alleles (So, Sp, Sq, Sr, Ss) were identified. The results obtained, together with information compiled from previous studies, allowed describing 12 new incompatibility groups (VIII-XIX). The self-incompatibility of several cultivars and the crosscompatibility among different incompatibility groups were verified by self-and cross-pollination experiments followed by observation of pollen tube growth. Five cultivars behaved as self-compatible, but two of them do not have the Se allele, which has been correlated with selfcompatibility. Thus, additional sources of self-compatibility different from Se appear to be involved in Japanese plum self-compatibility.

show abstract

“… Pedigree (Byrne 1989, Okie and Weinberger 1996; Brooks and Olmo 1997,Boonprakob et al. 2001, Goulão et al.…”

Section: Resultsmentioning

confidence: 99%

S‐RNase genotyping and incompatibility group assignment by PCR and pollination experiments in Japanese plum

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Numbers above the branches are bootstrap values derived from 1,000 heuristic replicates. Clades defined A, B, C and D are discussed in the text may have occurred more than once (Boonprakob et al 2001), the earliest cultivar being known two millenia ago in China (Faust and Surányi 1999).…”

Section: Discussionmentioning

confidence: 99%

“…Prunus salicina Lindl., reported to grow wild or to have been naturalised in Northern and South Eastern China (Kovalev 1941;Krüssmann 1978), is thought to have originated in the Yangtze River basin (Faust and Surányi 1999). This species is one of the most important plum species in cultivation today, and many new plum varieties have been obtained by hybridisation between P. salicina and other diploid Prunus species (Bellini et al 1998;Boonprakob et al 2001). In contrast, Prunus ussuriensis Kovalev & Kostina, endemic to North East China and East Siberia, and Prunus sogdiana Vassilcz., which is found in Tien Shan, Central Asia, grow wild in the forests of those regions (Sumnievich 1955;Paulov 1966).…”

Section: Introductionmentioning

confidence: 99%

Phylogenetics of Eurasian plums, Prunus L. section Prunus (Rosaceae), according to coding and non-coding chloroplast DNA sequences

Reales

Tobutt²,

Rivera

2009

Tree Genetics & Genomes

View full text Add to dashboard Cite

The genus Prunus contains the subgenus Prunus incorporating the European plums (section Prunus), the North American plums (section Prunocerasus) and the apricots (section Armeniaca). In section Prunus, there are approximately 20 species, which occur in three levels of ploidy, diploid 2nDespite a clear distinction between section Prunus and the other sections, phylogenetic relationships between species within the section are unclear. We performed a phylogenetic analysis on members of the section Prunus and three outgroup species using sequence data from four single-copy phylogenetically informative chloroplast DNA regions (atpB-rbcL, matK, rpl16, and trnL-trnF). After alignment, the analysed regions totalled 4,696 bp of sequence, containing 68 parsimonyinformative sites and 14 parsimony-informative indels. Data were analysed using both maximum parsimony and Bayesian likelihood and phylogenetic trees were reconstructed. The analyses recovered trees with congruent topologies and similar levels of statistical support for relationships between taxa. They confirmed that species belonging to section Prunus form a monophyletic clade within Prunus. The section is resolved into four wellsupported clades, which correspond to the geographical distribution of the species. The hexaploid species could not be resolved into distinct species clades but formed a wellsupported group separate from the tetraploid species, highlighting the distinct evolutionary origins of the different polyploid groups. The close relationship between the hexaploids and Prunus divaricata, Prunus cerasifera and Prunus ursina indicates the former may have derived from an ancestor of P. cerasifera and its allies.

show abstract

“…(Okie & Ramming 1999). Isozyme genes (Byrne & Littleton 1988) and random amplified DNA polymorphism (RAPD) markers (Boonprakob et al . 2001) have already been used for the identification of genotypes and the study of genetic diversity in Japanese plum.…”

Section: Primer Sequences Annealing Temperature (Ta) Pcr Product Simentioning

confidence: 99%

Simple‐sequence repeat (SSR) markers of Japanese plum (Prunus salicina Lindl.) are highly polymorphic and transferable to peach and almond

Mnejja

García-Más

Howad

et al. 2004

Molecular Ecology Notes

137

View full text Add to dashboard Cite

Thirty‐five polymorphic microsatellites were developed using a CT/AG enriched genomic library of Japanese plum cv. Santa Rosa. Twenty‐seven of them detected a single locus and eight two or more loci. A high level of variability was observed in a set of eight cultivars for the 27 single‐locus microsatellites: 5.7 average number of alleles per locus; 73% mean observed heterozygosity and 74% discrimination power. Most SSRs were transferable to peach (85%) and almond (78%).

show abstract

Genetic Relationships among Cultivated Diploid Plums and Their Progenitors as Determined by RAPD Markers

Cited by 23 publications

References 32 publications

S‐RNase genotyping and incompatibility group assignment by PCR and pollination experiments in Japanese plum

S‐RNase genotyping and incompatibility group assignment by PCR and pollination experiments in Japanese plum

Phylogenetics of Eurasian plums, Prunus L. section Prunus (Rosaceae), according to coding and non-coding chloroplast DNA sequences

Simple‐sequence repeat (SSR) markers of Japanese plum (Prunus salicina Lindl.) are highly polymorphic and transferable to peach and almond

Contact Info

Product

Resources

About