Genetic relationships and diversity among wild Salvia accessions revealed by ISSR and SCoT markers

Self Cite

Section: Resultssupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Assessment of genetic diversity inTriticum urartuThumanjan ex Gandilyan accessions using start codon targeted polymorphism (SCoT) and CAAT-box derived polymorphism (CBDP) markers

Gholamian

Etminan

Changizi

et al. 2019

Self Cite

“…Both of these techniques indicated low genetic diversity at population level and high genetic diversity at species level, and showed a similar clustering of H. serrata populations. The present study showed that using independent data from two different techniques can present a more efficient and accurate way to survey the genetic variability of a given species [21].…”

Section: Usability Of the Used Techniquesmentioning

confidence: 83%

“…This technique was proposed to be used in conjunction with other DNA fingerprinting techniques for applications such as genetic analysis, and quantitative trait loci mapping, especially in laboratories with a preference for agarose gel electrophoresis [10]. Thus, the ISSR and SCoT markers have been successfully used for quantification of genetic diversity in different plants [17][18][19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and variation ofHuperzia serrata(Thunb. ex Murray) Trevis. population in Vietnam revealed by ISSR and SCoT markers

Minh

Tien

Hau

et al. 2019

Genetic diversity and variation of four naturally distributed Huperzia serrata populations in Vietnam were analyzed based on the DNA fingerprint data obtained by ISSR and SCoT techniques separately and combined together. Both of ISSR and SCoT markers showed that the genetic diversity in Vietnam was relatively high at population and species level, except for the Hoang Lien population. The genetic diversity parameters of the Hoang Lien population were Nei's gene diversity index (H e) ¼ 0.1436, Shannon index (I) ¼ 0.2161, percentage of polymorphic bands (PPB) ¼ 45.45%; of the Bach Ma population, H e ¼ 0.21, I ¼ 0.33, PPB ¼ 71.97%; of the Ngoc Linh population, H e ¼ 0.20, I ¼ 0.31, PPB ¼ 74.24%; of the Bidoup population, H e ¼ 0.19, I ¼ 0.30, PPB ¼ 74.24%; and at species level in Vietnam, H e ¼ 0.23, I ¼ 0.36, PPB ¼ 100%. The genetic differentiation was high with a value of G ST ¼ 0.19 and the number of migrants was estimated through gene flow as Nm ¼ 2.16 individuals per generation among populations. The results of analysis of molecular variance (AMOVA) indicated that 28 and 72% of the total variation were among and within populations, respectively. The genetic diversity and genetic variation parameters of the investigated populations depended on the type of techniques used for DNA fingerprinting and also on the nature of each population. However, there was a high correlation and general trends among the obtained data sets when using ISSR, SCoT and the combination of these techniques.

“…The Shannon diversity index values calculated from both TRAPs and CoRAPs were consistent with the species genetic variability level. Comparable PIC values have been reported with dominant markers like RAPD and AFLP in African plantain (Musa paradisiaca) [42], ISSRs and RAPDs in Salvia species [40], AFLP in wheat (Triticum aestivum) [43] and SCoT markers [44][45][46][47][48]. TRAP and CoRAP are relatively high-throughput PCRbased marker systems; they appear to be very efficient relative to other systems.…”

Section: Corap Analysismentioning

confidence: 86%

Genetic variability and relationships amongSalviaecotypes/species revealed by TRAP-CoRAP markers

Fabriki-Ourang

Yousefiazarkhanian

2018

In this study, target region amplification polymorphism (TRAP) and conserved region amplification polymorphism (CoRAP) markers were used for genetic diversity and relationship analysis of 25 Salvia ecotypes/species. Twelve TRAP and CoRAP primer combinations (four arbitrary primers and three fixed primers from Salvia miltiorrhiza expressed sequence tag sequences) amplified 180 loci, of which all were polymorphic. The obtained high average PIC and MI values revealed high capacity of TRAP and CoRAP primers to detect polymorphic loci among Salvia species. TRAPs and CoRAPs had similar efficiency in detecting polymorphisms with excellent ability to differentiate the closely related Salvia ecotypes. Based on both TRAP and CoRAP data, tetraploid Salvia virgata (2n ¼ 4x ¼ 32) showed the highest values for Na (1.17, 1.29), Ne (1.29, 1.35), Shannon index (0.26, 0.32) and heterozygosity (0.172, 0.212) in all 11 studied species. The Nei's genetic distances with TRAPs and CoRAPs ranged from 0.23 to 0.69 and 0.19 to 0.64, respectively. The tree pattern based on combined CoRAP þ TRAP data with the neighbour joining method clustered 25 Salvia species/ecotypes into five major groups that showed good alignment with the population structure analysis results. In both TRAPs and CoRAPs data analysis of molecular variance (AMOVA), the estimated variance within Salvia species was greater than that among Salvia species.